University of Pennsylvania
Tel:
(215) 573-7407
Fax:
(215) 573-9327
E-mail: dnaseq@mail.med.upenn.edu
Web: www.med.upenn.edu/dnaseq/
DNA SEQUENCING FACILITY
FTP: dnaseq.med.upenn.edu
Mailing Address:
B1 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104
Request For Ion Torrent Proton Sequencing Service
PI ____________________________
Fund/PO No __________________________
Tel____________________
Date__________
Contact person (full name) ________________________
Tel ______________________
*E-mail (Required) ___________________________________ Fax ________________________
Ion Torrent Proton, an upgrade of PGM, combines pre-existing semiconductor technology with a
simple sequencing chemistry ( http://www.iontorrent.com ). Currently PI chip is available, PII and PIII will be
released in the near future.
Applications on PI chip: Targeted Sequencing of custom and ready-to-use gene panels e.g.
Comprehensive Cancer Panel & Inherited Disease Panel; RNA-Seq and AmpliSeq Transcriptome;
Exome & AmpliSeq Exome
Only 1 sample is run on a chip. No waiting is necessary to fill up a plate/flow cell/slide.
ExpEExpected Run Results (200b sequencing) and Cost (effective June 1, 2015)
Chip
PI
Average Read Length
Up to 200b
No of Reads
70 – 90M
Throughput
10 – 12 Gb
$1,760: 1 sample or 1 pool submitted – 1 run only
$1,600: 2 samples or 2 pools submitted – 2 runs
§ Sequencing Cost/Run
(pooled libraries submitted)
Barcoded library, gDNA or long amplicons, with Pippin Prep size selection,
1 – 3 libraries - $100 each; 4 or more libraries - $75 each
Barcoded Cancer Hotspot Panel v2 library,
4 minimum - $170 each, more for fewer samples
Comprehensive Cancer Panel or Custom Panel Library - Contact us
RNA-Seq & AmpliSeq Transcriptome Library - Contact us
Exome & AmpliSeq Exome Library - Contact us
Volume discount - Contact us
§ The sequencing price includes emPCR, sequencing, and preliminary data analysis to provide
FASTQ, BAM and BAI files, as well as sequence alignment and variant calls.
Library prep & QC not included.
Note – Downstream data analysis (provided after consultation with the user): $100/hr
08/1/15
Ion Torrent Proton Service
Please Fill OutTemplate
Template
Name
Type
Size
Amount**
Concentration
(µg)
(ng/µl)
Sequencing primer
1.
2.
3.
4.
5.
** gDNA or pooled amplicons
Required 300 - 400 ng by fluorometry or 1 – 1.5 µg by Nanodrop, Volume 30 – 50 µl
** Amplicon library with Ion adaptors attached
Required 100 – 200 ng by fluorometry or 300 - 600 ng by Nanodrop, Volume 30 –50 µl
Please Check the FollowingGel Picture Submitted (
)
Quantitation Method: Nanodrop (
Samples Pooled:
)
Barcoded:
(
Throughput Desired for Each Sample
Use PI chip (
)
(
) or Fluorometry (
)
)
No of reads _______________, No of bases _____________
Use 200 b kit (
)
Note: Please mention barcode sequences in the project description if different from Ion Torrent’s.
Project description with a title (required)
Use separate page if necessary
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08/1/15