University of Pennsylvania
Tel:
(215) 573-7407
Fax:
(215) 573-9327
E-mail: dnaseq@mail.med.upenn.edu
Web: www.med.upenn.edu/dnaseq/
DNA SEQUENCING FACILITY
Mailing Address:
B1 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104
FTP: dnaseq.med.upenn.edu
Request For Ion Torrent PGM Sequencing Service
PI ____________________________
Fund/PO No __________________________
Tel____________________
Date__________
Contact person (full name) ________________________
Tel ______________________
*E-mail (Required) ___________________________________ Fax ________________________
Ion Torrent PGM sequencer combines pre-existing semiconductor technology with a simple sequencing
chemistry ( http://www.iontorrent.com ). The sequencer is essentially a solid state pH meter. Known for scalability,
simplicity and speed, this inexpensive technology is really moving fast to achieve new goals in terms of throughput
and read length.
Applications include: Targeted resequencing of barcoded samples; Sequencing of captured
library(captured region 100 Kb to 5 Mb) using 316 & 318 chip; Sequencing of bacterial and viral genomes;
Sequencing of metagenomic samples (16S library & shotgun metagenomics) ; RNA-seq specially small RNA
sequencing; Validation of sequence data obtained on other platforms.
Only 1 sample is run at a time. No waiting is necessary for a plate/flow cell/slide to fill up.
ExpeExpected Run Results (200 and 400b sequencing) and Cost (effective Mar 1, 2014)
314 chip
Read Length
316 chip
318 chip
200b
400b
200b
400b
200b
400b
* No of Reads
> 100K
> 100K
> 1M
> 1M
~5 M
~5 M
* Throughput
¶ gDNA and long
amplicons
(includes 1 library)
> 20 Mb
> 40 Mb
> 200 Mb
> 400 Mb
~ 1 Gb
~ 2 Gb
$600
$725
$800
$925
$995
$1,120
§ Amplicons
Cancer panel v2
(includes 1 library)
Comprehensive
cancer panel
$515
$640
$720
$845
$920
$1,045
Barcoded library
$700
$900
Contact us
$85 each, gDNA or long amplicons, 4 minimum
$175 each, Cancer panel v2 or custom panel, 4 minimum
Barcoded library
* The throughputs on 314 and 316 chips are 2-3 fold higher than specifications. For 318 chip it’s slightly
higher.
¶ The price includes 1 library prep (for gDNA, long amplicons and AmpliSeq panels), sample evaluation,
emPCR, sequencing on a chip, and preliminary data analysis to provide FASTQ, BAM and BAI files.
§ The price does not include the preparation of amplicon library with Ion adaptors but includes sample evaluation.
03/01/14
Ion Torrent PGM service
Please Fill OutTemplate
Template
Name
Type
Size
Amount**
Concentration
(µg)
(ng/µl)
Sequencing primer
1.
2.
3.
4.
5.
** gDNA or pooled amplicons
Required 300 - 400 ng by fluorometry or 1 – 1.5 µg by Nanodrop, Volume 30 – 50 µl
** Amplicon library with Ion adaptors attached
Required 100 – 200 ng by fluorometry or 300 - 600 ng by Nanodrop, Volume 30 –50 µl
Please Check the FollowingGel Picture Submitted (
)
Quantitation Method: Nanodrop (
Samples Pooled:
)
Barcoded:
(
Throughput Desired for Each Sample
(
) or Fluorometry (
)
)
No of reads _______________, No of bases _____________
Use 314 chip (
)
316 chip (
)
Use 200 b kit (
)
400 b kit (
)
318 chip (
)
Note: Please mention barcode sequences in the project description if different from Ion Torrent’s.
Project description with a title (required)
use separate page if necessary
--------------------
03/01/14