GCC BIOTECH (INDIA) PVT. LTD.
NGS SEQUENCING FACILITY
Tel:
(033) 2357-6575
E-mail: ngs@gccbiotech.co.in
Web: www.gccbiotech.co.in
Mailing Address:
GCC BIOTECH (INDIA) PVT. LTD.
Patharberia Road,
Vill – Joychandipur, P.O.- Bakrahat,
P.S.- Bishnupur, Dist.- 24Pgs(South),
West Bengal - 743377, India
Off. no. +91 33 24951044
Request for Ion Torrent PGM Sequencing Service
(Please fill up by hand writing)
Sequencing kits of both 200 b & 400 bp read lengths are available.
PI ____________________________
Tel____________________
Contact person (full name) ________________________
E-mail (Required) ___________________________________
Fund/PO No __________________________
Date__________
Tel ______________________
Fax ________________________
Ion Torrent PGM sequencer combines pre-existing semiconductor technology with a simple sequencing chemistry (
http://www.iontorrent.com ). The sequencer is essentially a solid state pH meter. Known for scalability, simplicity and
speed, this inexpensive technology is really moving fast to achieve new goals in terms of throughput
and read length.
Applications include: Targeted resequencing of barcoded samples; Sequencing of captured library(captured region 100 Kb
to 1 Mb); Sequencing of bacterial and viral genomes; Sequencing of metagenomic samples; RNA-seq specially small RNA sequencing;
Validation of sequence data obtained on other platforms.
Expected Run Results
314 chip
316 chip
318 chip
Read Length
200b
400b
200b
200b
400b
No of Reads
> 100,000
> 100,000
> 1M
~5 M
~5 M
Throughput
> 20 Mb
> 40 Mb
> 200 Mb
~ 1 Gb
~ 2 Gb
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Please Fill OutTemplate
Template
Name
Size
Amount**
Type
Concentration
(µg)
Sequencing primer
(ng/µl)
1.
2.
3.
4.
5.
** gDNA or pooled amplicons
Required 300 - 400 ng by fluorometry or 2 – 2.5 µg by Nanodrop, Volume 30 – 50 µl
** Amplicon library with Ion adaptors attached
Required 100 – 200 ng by fluorometry or 300 - 600 ng by Nanodrop, Volume 30 –50 µl
Please Check the FollowingGel Picture Submitted ( )
Samples Pooled: ( )
Quantitation Method: Nanodrop ( ) or Fluorometry ( )
Barcoded:
Throughput Desired for Each Sample
Use 314 chip ( )
( )
No of reads _______________, No of bases _____________
316 chip ( )
Use 200 b kit ( )
318 chip ( )
400 b kit ( ), 314 and 318 chips only
Note: Please mention barcode sequences in the project description if different from Ion Torrent’s.
Project description with a title (required)
use separate page if necessary
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