University of Pennsylvania
Tel:
(215) 573-7407
Fax:
(215) 573-9327
E-mail: dnaseq@mail.med.upenn.edu
Web: www.med.upenn.edu/dnaseq/
DNA SEQUENCING FACILITY
Mailing Address:
B1 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104
FTP: dnaseq.med.upenn.edu
Request For Ion Torrent Proton Sequencing Service
PI ____________________________
Fund/PO No __________________________
Tel____________________
Date__________
Contact person (full name) ________________________
Tel ______________________
*E-mail (Required) ___________________________________ Fax ________________________
Ion Torrent Proton, an upgrade of PGM, combines pre-existing semiconductor technology with a simple
sequencing chemistry ( http://www.iontorrent.com ). The sequencer is essentially a solid state pH meter. Known for
scalability, simplicity and speed, this inexpensive technology is really moving fast to achieve new goals in terms of
throughput and read length.
Applications on PI chip include: AmpliSeq Transcriptome and Total RNA-Seq; AmpliSeq Exome and
TargetSeq Exome; Large Targeted Gene Panel e.g. Comprehensive Cancer Panel, Inherited Disease Panel
and Custom Panel.
Only 1 sample is run at a time. No waiting is necessary for a plate/flow cell/slide to fill up.
ExpEExpected Run Results (200b sequencing) and Cost (effective Oct 1, 2014)
Chip
PI
Average Read Length
Up to 200b
60 – 80M
No of Reads
Throughput
§ Sequencing
(pooled libraries submitted)
10 – 12 Gb
$1,100 (introductory)
Barcoded library, gDNA or long amplicons, with Pippin Prep size selection,
5 minimum - $85 each
Barcoded library, Cancer Hotspot Panel v2, 5 minimum - $175 each
Comprehensive Cancer Panel or Custom Panel Library - Contact us
AmpliSeq Transcriptome and Total RNA-Seq Library - Contact us
AmpliSeq Exome and TargetSeq Exome Library - Contact us
Volume discount - Contact us
§ The sequencing price includes emPCR, sequencing on a chip, and preliminary data analysis to
provide FASTQ, BAM and BAI files. It does not include library prep and QC.
Note - Data analysis is provided after consultation with the user at an additional cost of
$100/h
03/15/15
Ion Torrent Proton Service
Please Fill OutTemplate
Template
Name
Type
Size
Amount**
Concentration
(µg)
(ng/µl)
Sequencing primer
1.
2.
3.
4.
5.
** gDNA or pooled amplicons
Required 300 - 400 ng by fluorometry or 1 – 1.5 µg by Nanodrop, Volume 30 – 50 µl
** Amplicon library with Ion adaptors attached
Required 100 – 200 ng by fluorometry or 300 - 600 ng by Nanodrop, Volume 30 –50 µl
Please Check the FollowingGel Picture Submitted (
)
Quantitation Method: Nanodrop (
Samples Pooled:
)
Barcoded:
(
Throughput Desired for Each Sample
Use PI chip (
)
(
) or Fluorometry (
)
)
No of reads _______________, No of bases _____________
Use 200 b kit (
)
Note: Please mention barcode sequences in the project description if different from Ion Torrent’s.
Project description with a title (required)
Use separate page if necessary
--------------------
03/15/15