University of Pennsylvania
Tel:
(215) 573-7407
Fax:
(215) 573-9327
E-mail: dnaseq@mail.med.upenn.edu
Web: www.med.upenn.edu/dnaseq/
DNA SEQUENCING FACILITY
FTP: dnaseq.med.upenn.edu
Mailing Address:
B1 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104
Request For Ion Torrent PGM Sequencing Service
PI ____________________________
Fund/PO No __________________________
Tel____________________
Date__________
Contact person (full name) ________________________
Tel ______________________
*E-mail (Required) ___________________________________ Fax ________________________
Ion Torrent PGM sequencer combines pre-existing semiconductor technology with a simple sequencing
chemistry ( http://www.iontorrent.com ). The sequencer is essentially a solid state pH meter. Known for scalability,
simplicity and speed, this inexpensive technology is really moving fast to achieve new goals in terms of throughput
and read length.
Applications include: Targeted resequencing of barcoded samples; Sequencing of captured
library(captured region 100 Kb to 5 Mb) using 316 & 318 chip; Sequencing of bacterial and viral genomes;
Sequencing of metagenomic samples (16S library & shotgun metagenomics) ; RNA-seq specially small RNA
sequencing; Validation of sequence data obtained on other platforms.
Only 1 sample is run at a time. No waiting is necessary for a plate/flow cell/slide to fill up.
ExpeExpected Run Results (200 and 400b sequencing) and Cost (effective Sep 1, 2014)
314 chip
Read Length
* No of Reads
316 chip
318 chip
200b
400b
200b
400b
200b
400b
> 100K
> 100K
> 1M
> 1M
~5 M
~5 M
* Throughput
> 20 Mb > 40 Mb > 200 Mb > 400 Mb
~ 1 Gb
~ 2 Gb
§ Sequencing
(pooled libraries
$590
$750
$790
$950
$990
$1,150
submitted)
Barcoded library, gDNA or long amplicons, with Pippin Prep size selection,
5 minimum - $85 each
Barcoded library, cancer hotspot panel v2, 5 minimum - $175 each
Comprehensive cancer panel or custom panel library - Contact us
Volume discount - Contact us
* The throughputs on 314 and 316 chips are 2-3 fold higher than specifications. For 318 chip it’s slightly
higher.
§ The sequencing price includes emPCR, sequencing on a chip, and preliminary data analysis to provide
FASTQ, BAM and BAI files. It does not include library QC.
09/01/14
Ion Torrent PGM service
Please Fill OutTemplate
Template
Name
Type
Size
Amount**
Concentration
(µg)
(ng/µl)
Sequencing primer
1.
2.
3.
4.
5.
** gDNA or pooled amplicons
Required 300 - 400 ng by fluorometry or 1 – 1.5 µg by Nanodrop, Volume 30 – 50 µl
** Amplicon library with Ion adaptors attached
Required 100 – 200 ng by fluorometry or 300 - 600 ng by Nanodrop, Volume 30 –50 µl
Please Check the FollowingGel Picture Submitted (
)
Quantitation Method: Nanodrop (
Samples Pooled:
)
Barcoded:
(
Throughput Desired for Each Sample
(
) or Fluorometry (
)
)
No of reads _______________, No of bases _____________
Use 314 chip (
)
316 chip (
)
Use 200 b kit (
)
400 b kit (
)
318 chip (
)
Note: Please mention barcode sequences in the project description if different from Ion Torrent’s.
Project description with a title (required)
use separate page if necessary
--------------------
09/01/14