Preparation of 0.5 X TBE Buffer
-We use 0.5 X TBE Buffer for agarose gel electrophoresis in our lab. The following protocol can
be used to prepare 5 gallons:
Mix the following in a 2 L flask:
-108 g Tris base
-55 g Boric acid
-40 ml 0.5 M EDTA pH 8.0
-H2O to 2 L
Place the flask on a magnetic stirrer until completely dissolved. This results in a 5X concentrated
stock of TBE Buffer. Dilute the 2L of 5X stock by 10 in distilled water (add 18 L or 4.5 gallons of
distilled water). Mix well. The buffer is ready for use.
-Modified from Short Protocols in Molecular Biology, 5th edition
Aguirre Lab DNA TBE Buffer Prep Protocol
Last Updated: Apr/28/10