(24)Before you arrive for the Survey of Prokaryotes lab, please 1. Read the lab thoroughly, noting all safety guidelines. 2. Answer these preparatory questions: What safety procedures should you be sure to follow during this lab period? _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ What sort of evidence led to the reorganization of the taxonomy of prokaryotes in 1996? ______________________________________________________________________ Describe the general nature of the habitats of organisms in domain Archaea. ______________________________________________________________________ What is the other domain of prokaryotes?__________________________________ When a taxonomic scheme is established for a species or for groups of species, is it permanent? If not, what can change established taxonomy? ______________________________________________________________________ ______________________________________________________________________ If you are examining an unknown cell through the microscope, list three ways that distinguish it as a prokaryote and not a eukaryote. ______________________________________________________________________ How would Bacillus anthracis appear different from Spirillum volutans? Do all bacterial names reflect the appearance of the bacteria as these do? ______________________________________________________________________ ______________________________________________________________________ How is binary fission different than mitosis? ______________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ How might conjugation benefit bacterial survival? ______________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ Gram negative bacteria are generally more resistant to antibiotics than Gram positive bacteria. Using Figure 24.6 as a guide, what structure in Gram negative bacteria (but not Gram positive bacteria) supports this idea? ______________________________________________________________________ How does oil help in the visualization of structure through the microscope? ______________________________________________________________________ Can you ever see bacteria with the naked eye? Explain your answer. ______________________________________________________________________ How do bacteria make nitrogen available to living things? ______________________________________________________________________ Below is a diagram of 4 different antibiotics (numbered 1-4 and shown as a in the diagram) that are being tested in a petri dish that has been inoculated with bacteria. After a sufficient incubation period, bacterial growth occurred over the plate in all areas except in the zones of inhibition (shown with in the diagram) around the antibiotics. Would you choose antibiotic 1, 2, 3, or 4 to limit the growth of this bacterium?____________ 1 an ti bi 2 4 ot ic3 s (s h o be reasonable to conclude that the most From the results shown here, would it w effective antibiotic in this example would be an effective antibiotic against any n bacterium? Why or why not? wi th ______________________________________________________________________ in th Do cyanobacteria have chloroplasts?______________________________________ e di and are charged with the duty of its You are presented with an unknown cell, identification. As you study the cell, ag you note that it lacks a nucleus, it has ra unusual cell walls, and that is rRNA is distinctly different. To which domain m would you assign it? ). _____________________________________________________________________ List five human disease that are caused by bacteria. Include the name of the disease and the name of the bacteria that cause the disease. Human Disease Bacterial cause of the disease Is boiling water an effective way to kill all bacteria? Explain your answer. _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ Write an equation for nitrogen fixation that shows the reagents, enzyme, and product. _____________________________________________________________________