MLAB 2434
Microbiology
Chapter 1: Bacterial Cell Structure, Physiology,
Metabolism & Genetics
Learning Objectives
1.
Describe microbial classification and apply the rules of scientific
nomenclature for bacterial names.
2.
Differentiate between prokaryotic and eukaryotic cell types,
including their cytoplasmic and cell envelope structures and
functions.
Describe the cell walls of gram positive and gram negative
bacteria. Explain the Gram stain reaction of each cell wall type.
Describe two other bacterial cell wall types, and give microbial
examples of each.
3.
4.
Explain the use of the following stains in the diagnostic
microbiology laboratory: Gram stain, acid-fast stains,
lactophenol cotton blue, methylene blue and India ink.
5.
List the nutritional and environmental requirements for
bacterial growth and define the categories of media used for
culturing bacteria in the laboratory.
6.
Define the atmospheric requirements of obligate aerobes,
microaerophiles, facultative anaerobes, obligate anaerobes, and
capnophilic bacteria.
7.
Describe the stages in the growth of bacterial cells.
8.
Describe the importance of microbial metabolism in clinical
microbiology.
9.
Differentiate between fermentation and oxidation (respiration).
10. Name the three (3) biochemical pathways that bacteria use to
convert glucose to pyruvate.
11. Discuss the development and transfer of antibiotic resistance in
bacteria.
12. Define the various categories of temperature requirements for
micro-organisms.
2/9/2016
MLAB 2434
Microbiology
13. Identify three (3) miscellaneous structures found in or on
bacterial cells and state their function.
2/9/2016