PBL session: Cellular players and compartments in lymphoid tissues during
immune responses.
(Regulation of TH2 development by CXCR5+ dendritic cells and
lymphotoxin-expressing B cells. Beatriz León, et al., 2012, Nature Immunology, 13,
681–690.)
Instructor: Dr. MC Hsu (meichihsu@mail.ncku.edu.tw)
Deadline: 12/24 (Mon) 4:30pm
(IMM Group 4)
 Please describe the events of an immune response chronologically, and the cellular
players involved.
 Please describe the cellular compartments of lymphoid tissues (in steady state and
under an immune response), using the spleen and the LN as examples.
 Please explain Figure 1 and describe the novel findings.
(IMM Group3)
 Please describe the mechanisms of antigen presentation, the types of cells with the
ability to present antigens, and compare those cells.
 Please describe the types of dendritic cells and the markers used to
identify/characterize their differentiation status.
 Please explain Figure 2 and describe the novel findings.
(IMM Group 2)
 Please describe the chemokine gradient maintaining the cellular compartments in
lymphoid tissues.
 Please describe how lymphocytes are able to migrate along the chemokine gradient
during an immune response.
 Please explain Figure 3 and describe the novel findings.
(IMM Group 1)
 Please explain Figure 4, the novel findings and the mouse model used in this set of
data.
 Please describe the differentiation and functions of Tfh cells.
 Please explain Figure 5, the novel findings, the mouse model used in this set of
data and compare it to that in Figure 4.
YOU CAN EARN BONUS POINTS BY ANSWERING THE FOLLOWING
(please send me the answers as groups):
 Why is MD4MT used in Figure 7?
 Why is the mouse model described in Figure 8 used?
 Why is CD45.1 B cells not used in the mouse model described in Figure 8?