Principal Investigator/Program Director (Last, First, Middle):
Levey, Allan, I
IMAGING CORE RESOURCES
FACILITIES: Specify the facilities to be used for the conduct of the proposed research. Indicate the performance sites and describe capacities, pertinent capabilities, relative proximity, and extent of availability to the project. If research involving Select Agent(s) will occur at any performance site(s), the biocontainment resources available at each site should be described. Under “Other,” identify support services such as machine shop, electronics shop, and specify the extent to which they will be available to the project. Use continuation pages if necessary.
Laboratory:
The imaging core facility resides on the 5 th floor of the Whitehead Building in about 350 sq feet of reconfigured space with adequate ventilation and cooling for the microscope and an adjacent workroom with workstations for image analysis. An additional ~125 sq feet are available for the new confocal microscope.
Clinical:
Animal:
Computer:
The PI has a computer in his office and the Imaging facility has several computer workstations available for image analysis.
Office:
The PI has an office on the 4 th floor of the Whitehead Building.
Other:
MAJOR EQUIPMENT: List the most important equipment items already available for this project, noting the location and pertinent capabilities of each.
The CND houses a Zeiss LSM 510 NLO META system which will be available in the Imaging Core for stateof-the-art confocal microscopy, which uses a Zeiss Axiovert 100M inverted microscope. The illumination system incorporates one argon and two helium-neon lasers for single-photon excitation at 458, 488, 514,
543, and 633 nm, and a Coherent tunable titanium-sapphire laser for two-photon excitation (700-900 nm).
The META spectral imaging system plus two conventional photomultiplier detectors offer simultaneous multichannel color display of fluorescence. The 32-channel META spectral detector allows the separation of signals with extremely overlapping emission profiles, heretofore impossible using glass filters. Workstations are available for capturing, analyzing, and printing high quality digital images. A Pixcell II laser capture microdissection system is available for collecting single cells, cell layers, or groups of cells from tissue sections or cell cultures.
A second Zeiss LSM510 META, 2-photon system has been added since our previous submission of this application and is also available for use in the CND. The META feature allows for spectral unmixing which provides one to distinguish between overlapping emission, such as GFP and YFP. The two-photon laser allows deeper imaging within a tissue.
Funds have been obtained to support the purchase of a third confocal microscope to relieve pressure on usage of the two LSM510’s by providing greater access for researchers to confocal microscopes, minimize delays in getting microscope time, and provide better data collection for live cell imaging. In May, 2007 the
Imaging Core will acquire a new Nikon Livescan SFC confocal TE2000 microscope equipped with four laser lines, DIC, CCD camera, automated stage with auto-focus for multi-point sampling, and environmental chamber for long term data collection of live cells, all controlled by NIS-Elements software running on 3.4
GHz Dual Core Pentium D CPU.
PHS 398 (Rev. 04/06) Page Resources Format Page