SCCC Microarray Core Facility presents a seminar on

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SCCC Microarray Core Facility presents a seminar on:

Substrates, Scanner Calibration and Gene Expression Calibration

Standards

Youxiang Wang, Ph.D., Chief Executive and Co-founder

Full Moon BioSystems, Inc.

845 W. Maude Ave, Sunnyvale, CA 94085

Tel: 408-212-4008, Fax: 408-749-0998

Email: ywang@fullmoonbiosystems.com

, web: www.fullmoonbiosystems.com

Microarray technology has revolutionized the study of molecular biology. This will allow the realization of the greatest potential applications to diagnostics. When used in diagnostics, microarray has to meet a high degree of reproducibility, reliability and quality in order to become a standard tool. Currently, the reproducibility and reliability of quantitative microarray results are not sufficiently robust to permit widespread application of this technology in diagnostic and screening areas. Therefore standards are urgently needed. These standards should address two issues: the quantitation of fluorescence using microarray readers and the quantitation of biological samples. To ask a simple question, how does one know that the cy3 and cy5 fluorescent intensity ratio in the differential gene expression array experiment reflects the true expressed gene ratio. The equal amount of cy3 and cy5 labeled probes are mixed and spotted on slides. The resulting cy3 and cy5 fluorescent intensity ratio is not one. The ratio is actually changing if one mixes the equal amount of cy3 and cy5 at different concentrations. Keeping the cy3 labeled probe concentration fixed and changing cy5 labeled probe concentration, the resulting solutions are spotted and scanned. The cy3 and cy5 fluorescent intensity ratio from the scanner does not correlate with the cy3 and cy5 concentration ratio in solution. That means that the fluorescent intensity ratio of cy3 and cy5 from the scanner data is not correlated with expressed gene ratio. Therefore calibration is needed. The expression calibration slides developed by Full Moon

BioSystems are capable of being used for calibration scanners and quantifying the expressed gene ratio.

Therefore, the expression calibration slides are essential tools to facilitate the comparison, interpretation, and validation of microarray data.

Furthermore, we have developed a novel nano-well reaction coated surface for manufacturing DNA arrays, protein arrays, antibody arrays and peptide arrays. This porous 3D nano-well surface is sensitive enough to immobilize cell secret proteins or cell lysis for detection relative protein expression level without advanced separation or concentration by using dye-labeled antibody directly.

This presentation will focus on discussion on how to use our expression calibration slides as an emerging technology to standardize or normalize microarray data and how to use the novel coating chemistry to improve array quality and

Thursday October 07, 2004

7 Medical Park, 6

th

Floor, Mandanas Classroom

12: 30 pm

Questions? Contact: Iram Quraishi Ph: 803-434-3579

Email:

irishi@gw.mp.sc.edu

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