UOW Laboratory Safety 2000
UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
***** APPENDIX 4 - CHEMICAL SAFETY *****
Sheet........1... of...1.........
Title of Experiment
1.
Room/Laboratory
3BCM519 Analytical
Methods
SDS-PAGE
ELECTROPHORESIS
7th Floor Teaching
Laboratory
Site
NCS
Brief description of work
Students determine the molecular mass of an unknown protein by Sodium Dodecyl Sulphate Polyacrylamide
Gel Electrophoresis (SDS-PAGE).
Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually required. Note
also that it is RISK that is to be assessed, not HAZARD.
2.
LIST OF SUBSTANCES USED OR CREATED (continue on a separate sheet if necessary)
Manufacturers Safety Data
Computer Data Base
Product Label
Other (Specify)
NAME OF SUBSTANCE
(CODE)
PHYSICAL
DESCRIPTION
MAX.
QUANTITY
EXPOSED TO
DATA AVAILABLE
(YES/NO)
Coomassie Blue R-250
Bluecoloured
liquid in
Methanol/wa
ter/acetic
acid
ml
Y
Methanol
Colourless
liquid
ml
Y
Acetic Acid
Colourless
liquid
ml
Y
2-Mercaptoethanol
Colourless
liquid
< ml
Y
0.192 M Tris-HCl, 25 mM
Glycine Buffer, pH 8.3
Colourless
liquid
~ 500 ml
Y
0.1% Sodium Dodecyl
Sulphate (0.1 %SDS)
White
powder or
colourless
liquid
< ml
Y
Polyacrylamide Gel
Solid gel
encased
between
plastic plates
<g
Y
KNOWN HAZARDS
(See Note;
use numbers)
4 (Toxic)
5 (Harmful)
6 (Corrosive)
7 (Irritant)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin and
Eyes)
3 (Flammable)
4 (Toxic)
7 (Irritant)
12 (Inhalation)
13 (Ingestion)
15 (Skin and
Eyes)
3 (Flammable)
5 (Harmful)
6 (Corrosive)
15 (Skin and
Eyes)
3 (Flammable)
4 (Toxic)
5 (Harmful)
7 (Irritant)
15 (Skin and
Eyes)
4 (Toxic)
7 (Irritant)
10 (Teratogen)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin or Eyes)
5 (Harmful)
7 (Irritant)
15 (Skin or Eyes)
Handle with care
as gel may
contain
acrylamide
monomer (see
below)
UOW Laboratory Safety 2000
Molecular weight markers
(pre-stained)
Laemmli sample buffer
Sample buffer (containing
0.1% Bromophenol Blue and
Glycerol)
Bluecoloured
liquid
Blue
coloured
liquid
< ml
N
Exercise due
care
< ml
Y
4 (Toxic)
5 (Harmful)
7 (Irritant)
14 (Skin
absorption)
15 (Skin or Eyes)
NOTE: 1) EXPLOSIVE
2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE
7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION
13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT
18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
3.
PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)
NAME/CLASS
INITS
3BCM519 Class
Curley
4.
Ref Note 17, 18: see EH40 (current edition).
P
STATUS
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Students
Once per year
Senior
Lecturer
Occasionally
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES
(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):
Technicians.
State control measures (to prevent inappropriate persons/groups having access to the substances):
Reagents are only made available to students on day of laboratory session. Once the practical session is completed
reagents should be either disposed of or stored away by the technical staff.
5.
EMERGENCY PROCEDURES (as per Hazard Data Sheet).
if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify bel ow action to be taken.
CODE
SPILLAGE/UNCONTROLLED RELEASE
None
FIRE
None
UOW Laboratory Safety 2000
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:
SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
Tris/Glycine Buffer, pH 8.3
Wash skin/eye wash
Methanol
Wash skin/eye wash*
Acetic Acid
Wash skin/eye wash*
2-Mercaptoethanol
Wash skin/eye wash*
0.1% SDS
Wash skin/eye wash*
Laemmli sample buffer
Wash skin/eye wash
Coomassie Blue stain (contains Methanol)
Wash skin/eye wash*
* In case of contact with eyes, flush with copious amounts of water for at least 15 min. Ensure adequate
flushing by separating eyelids with fingers. CONSULT A PHYSICIAN AS SOON AS POSSIBLE. If swallowed,
wash the mouth (if person is conscious) with copious amounts of water. Do NOT induce vomiting. CALL A
PHYSICIAN. In the case of Methanol ingestion, a PHYSICIAN should be called IMMEDIATLEY.
(NB. Antidotes and special treatment may be obtained through....
N/A...............................................................................................................................)
UOW Laboratory Safety 2000
6.
CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other necessary measures.
SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)
1. Limiting exposure time of personnel - state maximum time
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
4. Sufficient general ventilation
5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.)
* 6. Safe disposal/storage of hazardous substances
* 7. Regular cleaning of the workplace
* 8. Adequate washing facilities
* = MANDATORY
STORAGE - SAFETY CONSIDERATIONS
Methanol, -mercaptoethanol and acetic acid should
be stored in fireproof cupboards when not in use as
they are potential fire hazards.
SPECIFY
HANDLING PRECAUTIONS
When handling polyacrylamide gels, disposable
gloves should be worn as polyacrylamide gels may
contain acrylamide monomer (which is toxic as well
as a possible carcinogen). In addition, when
handling staining and de-staining solutions, gloves
should be worn as these solutions contain methanol
and acetic acid (see above).
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Buffer solutions, stain & de-stain may be safely disposed by washing down the drain, washing with copious
amounts of water.
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the exper iment)
(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)
N/A
8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)
(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)
Take care when running the electrophoretic separation due to the high voltages involved.
Always turn off the power and disconnect from mains before disassembling the electrophoresis apparatus.
9.
I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the
assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).
Name of Assessor:
Dr Paul Curley
Signed:
Status of Assessor:
Senior Lecturer
Date:
9th October, 2003