UOW Laboratory Safety 2000
UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
***** APPENDIX 4 - CHEMICAL SAFETY *****
Sheet........1... of...1.........
Title of Experiment
1.
Room/Laboratory
3BCM505 Proteins and
Enzymes PROTEIN
PURIFICATION
7th Floor Teaching
Laboratory
Site
NCS
Brief description of work
Students determine the specific activity of wheat germ acid phosphatase by carrying out a timed enzyme
assay and protein concentration determination using Coomassie blue (Bradford) reagent.
Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually required. Note
also that it is RISK that is to be assessed, not HAZARD.
2.
LIST OF SUBSTANCES
NAME OF SUBSTANCE
(CODE)
PHYSICAL
DESCRIPTION
MAX.
QUANTITY
EXPOSED TO
DATA AVAILABLE
(YES/NO)
Sodium Hydroxide (0.1 M)
Colourless
liquid
ml
Y
Sodium Acetate (0.05 M; pH
5.0)
White
powder or
colourless
liquid
White
powder or
colourless
liquid
White
powder or
cloudy liquid
mg
Y
mg
Y
White
powder or
colourless
liquid
Blue
coloured
liquid
mg
N
ml
Y
p-nitrophenyl phosphate
(0.01M)
Acid phosphatase (from
wheat germ)
Bovine Serum Albumin
Coomassie Blue (Bradford
Reagent)
mg
Y
NOTE: 1) EXPLOSIVE
2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE
7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION
13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT
18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
KNOWN HAZARDS
(See Note;
use numbers)
5 (Harmful)
6 (Corrosive)
7 (Irritant)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin and Eyes)
16 (Ingestion)
7 (Irritant)
14 (Skin
Absorption)
15 (Skin and Eyes)
7 (Irritant)
14 (Skin
Absorption)
15 (Skin and Eyes)
20 (Sensitization)*
* may cause
allergic reaction
upon prolonged or
repeated exposure
4 (Toxic)
5 (Harmful)
6 (Corrosive)
7 (Irritant)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin and eyes)
Ref Note 17, 18: see EH40 (current edition).
UOW Laboratory Safety 2000
3.
PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)
NAME/CLASS
INITS
STATUS
3BCM505 Class
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Once per year
Curley
Bucke
Perry
P
C
D
Lecturer
Lecturer
Lecturer
Occasionally
Occasionally
Occasionally
Thompson
S
Lecturer
Occasionally
4.
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES
(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):
Reagents are available to students (under supervision) and Technical/Stores staff only.
State control measures (to prevent inappropriate persons/groups having access to the substances ):
Reagents are only made available to students on the day of laboratory session. Once the practical session is
completed reagents are either disposed of or stored away by the technical staff.
EMERGENCY PROCEDURES (as per Hazard Data Sheet).
if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify below action to be taken.
CODE
SPILLAGE/UNCONTROLLED RELEASE
None
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:
FIRE
None
SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
Sodium Acetate
Wash skin/eye wash
p-nitrophenyl phosphate
Wash skin/eye wash
Acid phosphatase
Wash skin/eye wash
Bovine Serum Albumin
Wash skin/eye wash
Coomassie Blue (Bradford Reagent)
Wash skin/eye wash*
NaOH
Wash skin/eye wash**
* In case of contact with eyes, flush with copious amounts of water for at least 15 min. Ensure adequate
flushing by separating eyelids with fingers. CONSULT A PHYSICIAN AS SOON AS POSSIBLE. If swallowed,
wash the mouth (if person is conscious) with copious amounts of water. Do NOT induce vomiting. CALL A
PHYSICIAN.
** In case of contact with eyes, flush with copious amounts of water for at least 15 min. Ensure adequate
flushing by separating eyelids with fingers. CONSULT A PHYSICIAN AS SOON AS POSSIBLE.
(NB. Antidotes and special treatment may be obtained through............................. N/A......................................................................................................)
UOW Laboratory Safety 2000
6.
CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other necessary measures.
SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)
1. Limiting exposure time of personnel - state maximum time
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
4. Sufficient general ventilation
5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.)
* 6. Safe disposal/storage of hazardous substances
* 7. Regular cleaning of the workplace
* 8. Adequate washing facilities
* = MANDATORY
STORAGE - SAFETY CONSIDERATIONS
Commassie Reagent stock solutions should be kept
in fume hood when not is use.
SPECIFY
HANDLING PRECAUTIONS
Disposable rubber gloves and fume hood should be
used when dispensing large volumes (> 10 ml) of
Coomassie Reagent.
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Unused reagents can be safely disposed of down the drain. Wash sink with copious amounts of water.
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the exper iment)
(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)
8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)
(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)
None.
9.
I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the
assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).
Name of Assessor:
Dr Paul Curley
Signed:
Status of Assessor:
Senior Lecturer
Date: 27th
September, 2003