UOW Laboratory Safety 2000
UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
***** APPENDIX 4 - CHEMICAL SAFETY *****
Sheet........1... of...1.........
Title of Experiment
1.
Room/Laboratory
3BCM505 Proteins and
Enzymes PEPTIDE
SEQUENCING
7th Floor Teaching
Laboratory
Site
NCS
Brief description of work
Students separate and identify and unknown peptide fragments by TLC followed by identification of
individual amino acids by staining with Ninhydrin reagent.
Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually required. Note
also that it is RISK that is to be assessed, not HAZARD.
2.
LIST OF SUBSTANCES USED OR CREATED (continue on a separate sheet if necessary)
Manufacturers Safety Data
Computer Data Base
Product Label
Other (Specify)
NAME OF SUBSTANCE
(CODE)
Alanine
PHYSICAL
DESCRIPTION
MAX.
QUANTITY
EXPOSED TO
DATA AVAILABLE
(YES/NO)
White
powder or
colourless
liquid in
solution
White
powder or
colourless
liquid in
solution
mg
Y
mg
Y
Phenylalanine
White
powder or
colourless
liquid in
solution
mg
Y
Proline
White
powder or
colourless
liquid in
solution
White
powder or
colourless
liquid in
solution
mg
Y
mg
Y
1-Propanol
Colourless
liquid
ml
Y
Acetic Acid
Colourless
liquid
ml
Y
Lysine
Valine
KNOWN HAZARDS
(See Note;
use numbers)
4 (Toxic)
10 (Teratogen)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin or Eyes)
4 (Toxic)
10 (Teratogen)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin or Eyes)
4 (Toxic)
10 (Teratogen)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin or Eyes)
4 (Toxic)
10 (Teratogen)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin or Eyes)
4 (Toxic)
10 (Teratogen)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin or Eyes)
1 (Explosive)
3 (Flammable)
8 (Carcinogen)
9 (Mutagen)
10 (Teratogen)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin or Eyes)
1 (Explosive)
3 (Flammable)
5 (Harmful)
6 (Corrosive)
7 (Irritant)
12 (Inhalation)
13 (Ingestion)
14 (Skin
Absorption)
15 (Skin or Eyes)
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Ninhydrin Spray (0.2% in
acetone)
Colourless
liquid
ml
Y
Cellulose powder (TLC
plates)
White
powder
adsorbed
onto metal or
glass plates
Colourless
Liquid
< mg
N
ml
Y
Acetone
NOTE: 1) EXPLOSIVE
2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE
7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION
13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT
18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
3.
12 (Inhalation)
1 (Explosive)
3 (Flammable)
5 (Harmful)
12 (Inhalation)
13 (ingestion)
14 (Skin
Absorption)
15 (Skin and
Eyes)
Ref Note 17, 18: see EH40 (current edition).
PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)
NAME/CLASS
INITS
3BCM505 Class
4.
5 (Harmful)
7 (Irritant)
12 (Inhalation)
13 (Ingestion)
15 (Skin or Eyes)
STATUS
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Students
Once per year
Curley
P
Lecturer
Occasionally
Perry
D
Lecturer
Occasionally
Thompson
S
Lecturer
Occasionally
Bucke
C
Lecturer
Occasionally
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES
(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):
Technicians.
State control measures (to prevent inappropriate persons/groups having access to the substances ):
Reagents are only made available to students on day of laboratory session. Once the practical session is
completed reagents are either disposed of or stored away by the technical staff.
UOW Laboratory Safety 2000
5.
EMERGENCY PROCEDURES (as per Hazard Data Sheet).
if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify below action to be taken.
CODE
SPILLAGE/UNCONTROLLED RELEASE
None
FIRE
None
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:
SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
Alanine
Wash skin/eye wash
Lysine
Wash skin/eye wash
Phenylalanine
Wash skin/eye wash
Proline
Wash skin/eye wash
Valine
Wash skin/eye wash
1-Propanol
Wash skin/eye wash
Acetic Acid
Wash skin/eye wash
Ninhydrin Spray
Wash skin/eye wash
Acetone
Wash skin/eye wash
(NB. Antidotes and special treatment may be obtained through.......N/A............................................................................................................................)
6.
CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other necessary measures.
SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)
1. Limiting exposure time of personnel - state maximum time
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
4. Sufficient general ventilation
5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.)
* 6. Safe disposal/storage of hazardous substances
* 7. Regular cleaning of the workplace
* 8. Adequate washing facilities
* = MANDATORY
STORAGE - SAFETY CONSIDERATIONS
1-Proponal, Acetic Acid & Acetone should be stored
in fire-proof cupboards when not in use as they are
potential fire hazards.
SPECIFY
HANDLING PRECAUTIONS
Disposable rubber gloves and fume hood should be
worn when using Ninhydrin spray.
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Unused reagents can be safely disposed of down the drain.
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the experiment)
(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)
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8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)
(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)
During the course of the practical session students prepare thin capillary tubes for application of samples to
TLC plates using a Bunsen burner. Students MUST wear protective eye-glasses during this procedure and
excerise care when using the Bunsen burner (e.g. tie back long hair, keep clothes away from flame,
extinguish Bunsen Burner when no longer required, etc.).
9.
I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the
assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).
Name of Assessor:
Dr Paul Curley
Signed:
Status of Assessor:
Senior Lecturer
Date: 27th
September, 2003