ERIE COMMUNITY COLLEGE
NORTH CAMPUS
COURSE OUTLINE
A.
Unit Code:
Suggested Course Title:
2188
ML 212-Clinical Analysis III
B.
Curriculum/Program:
Clinical Laboratory Technician
C.
Catalog Description:
Discussion of sodium and potassium analysis, ion selective electrodes, electrophoresis,
plasma proteins, immunoassay, chromatography, drug analysis, endocrinology, iron
analysis, osmolality and automation, lactate and magnesium analysis.(time permitting)
Prerequisites: ML 122, ML 123, ML 124, ML 125, and ML 126)
Co-requisite: ML 213 or permission of instructor.
F (N)
D.
Duration of
Instructional Period:
50 minutes, 3 class meetings per week, 15 weeks
E.
Academic Credit Hours:
Contact Hours:
3.0
3.0
(3-0-3)
F.
Suggested Text/
Course Materials:
Clinical Chemistry, by Bishop, Fody, and
Schoeff
G.
Course Outcomes:
Upon completion of this course, the student will be able to:
1.
2.
3.
4.
5.
6.
4/2014
Describe basic principles of ion selective electrodes.
Discuss the clinical significance, specimen precautions and methods of analysis
for sodium, potassium, total serum proteins, albumin, and serum and urine
osmolality.
Identify and explain basic principles, technique, apparatus, and quantitation of
immunoassay, electrophoresis, iron analysis, chromatography and osmometry as
applied to electrolytes.
Detail clinical applications of immunoassay, electrophoresis, osmometry, and
chromatography.
Discuss the principles of continuous flow, discrete sample, centrifugal, and dry
chemistry analyzers.
Describe and contrast commonly used automated laboratory instruments.
1
H.
Program Competencies:
Upon graduation with an Associate in Applied Science Degree in Medical Laboratory
Technician, the graduate will be able to:
a.
b.
Perform all of the routine tests in a modern clinical laboratory. (Obj. 1, 2, 6)
Identify direct causes of technical or instrumental problems and make
appropriate corrections using preset strategies. (Obj. 1, 2, 3, 6)
Calculate the results of tests performed if necessary. (Obj. 2, 3)
Operate equipment or instruments necessary for routine analytical tests.
Recognize instrument malfunction and take appropriate action. (Obj. 1, 3, 6)
Perform, record and evaluate all quality control procedures required for the tests
assayed. (Obj. 2, 3, 6)
Recognize abnormal or unusual test results and follow institutional procedures
for reporting critical values. (Obj. 2, 4)
Perform and record routine instrument checks and maintenance procedures.
(Obj. 1, 3, 6)
Observe established safety measures. (Obj. 1, 2, 3, 6)
e.
g.
h.
i.
k.
l.
I.
SUNY General Education Knowledge and Skills Areas:
Not applicable to course offering in Health Science Division.
J.
ECC Learning Outcomes (LO):
1.
2.
3.
K.
Information Literacy (1-6)
Scientific Reasoning (1-6)
Quantitative Reasoning (2, 3)
Assessment of Student Learning:
K1. Evaluation of Student Learning: Course outcomes may be met by hourly
examinations, quizzes, selected homework assignments, oral presentations, case
evaluations and a written project. The final grade will be calculated as follows:






Hourly Examinations
Final Examination
Quizzes
Homework
Project
Attendance
30%
30%
10%
10%
15%
5%
K2. Assessment Of Student Learning: The methods of evaluation should be consistent
with the level of the course and meet criteria as set forth by the Accreditation Agencies
to include cognitive levels, I, II, and III, as well as, affective and psychomotor skills
appropriate to the course.
4/2014
2
L.
Library Resources:
Medical/Clinical Laboratory Technology reference books and journals particularly those
pertaining to Clinical Chemistry.
M.
Topical Outline:
Instructional Unit
4/2014
Instructional Period
1.
Sodium and Potassium Analysis
2 weeks
a.
Principles of ion selective electrodes.
b.
Measurement of sodium and potassium.
c.
Clinical significance and specimen precautions of sodium.
d.
Clinical significance and specimen precautions of potassium analysis.
2.
Electrophoresis
3 weeks
a.
Chemical and physical principles.
b.
Buffer pH and ionic strength.
c.
Processes affecting the separation pattern.
d.
Differentiation of support media.
e.
Quantitation.
f.
Clinical applications.
g.
Isoelectric Focusing; Immunoelectrophoresis; Capillary electrophoresis.
h.
Rapid Electrophoresis (REP) System.
3.
Serum Proteins
2 weeks
a.
Functions of the plasma proteins.
b.
Measurement and clinical significance of total serum proteins.
c.
Measurement and clinical significance of albumin.
d.
Serum protein electrophoresis.
4.
Immunoassay and Applications
3 weeks
a.
Basic principles and definitions.
b.
Outline of a basic immunoassay procedure.
c.
Labels used in immunoassay procedures.
d.
Competitive vs. noncompetitive immunoassays.
e.
Heterogeneous vs. homogeneous immunoassays.
f.
IRMA, EMIT, ELISA, Fluorescence, and Chemiluminescent
immunoassay procedures and instrumentation.
h.
Clinical applications - hormones, therapeutic drug monitoring and
toxicology.
5.
Iron analysis
1 Week
a.
Basic principles and definitions.
b.
Iron absorption, deficiency and toxicity.
c.
Laboratory evaluation of iron, including total iron, total iron capacity,
transferrin, and ferritin.
3
d.
e.
N.
4/2014
Sample collection, handling.
Calibration, quality control procedures.
6.
Chromatography
a.
Basic principles of thin layer chromatography.
b.
Basic principles of gas chromatography.
c.
Basic principles of liquid chromatography.
1 week
7.
Automation
a.
Basic Principles of Automation.
b.
Continuous flow analyzers.
c.
Discrete sample analyzers.
d.
Centrifugal analyzers.
e.
Dry Chemistry analyzers.
1 week
8.
Lactate and Magnesium
a.
Basic principles and definitions.
b.
Clinical applications.
c.
Laboratory evaluation of lactate and magnesium.
d.
Sample collection and handling.
e.
Calibration, quality control procedures.
1 week
9.
Final Exam
1 week
Proposal Prepared by:
Date Prepared:
Date Last Updated:
CLT/MA Faculty
April 2014
March 2008
4