LRI Genomics Core Facility
http://www.lerner.ccf.org/services/gc/
NE5-253, Phone: 216-444-0974
LERNER RESEARCH INSTITUTE
MiSeq Library Prep and Sequencing Request Form
Date: ______________________________________
Submitted by: ______________________________
PI Name: ___________________________________
CCF Email: ___________________________@ccf.org
PI Email: ___________________________________
Non-CCF Email: _____________________________
Phone number: _____________________________
Non-CCF Affiliation: _________________________
Department: _______________________________
Phone number: _____________________________
Activity or PO#: _________________________
Sample type: _____ mRNA, _____ small RNA, _____ High conc. DNA, _____ Low conc. DNA
Library preparation requested
(check one)
___ Bacterial Metagenomics
MiSeq Run Type
(check one)
___ 1x50 nt PE read
Multiplex run
(check one)
___ No multiplex (1 sample/lane)
___ RNA-Seq/RiboZero
___ 2x75 nt PE read
___ 2 samples per lane
___ Small RNA (microRNA)
___ 2x150 nt PE read
___ 3 samples per lane
___ Targeted RNA-Seq
___ 2x250 nt PE read
___ 4 samples per lane
___ Amplicon Sequencing
___ 2x300 nt PE read
___ Other*
*Please specify: __________
___
___ User prepared library*
*Library type: __________
*Kit used: _____________
samples per lane
Sample submission instructions: Please submit all samples on ice. Appropriate QC will be performed
Run type
Concentration required
Total volume required
Amplicon-Seq
5 ng amplicon DNA
15 ul
RNA-Seq
1-3 ug Total RNA
15 ul
Small RNA
2-5 mg of Total RNA
15 ul
ChiP-Seq
5-10 ng DNA
50 ul
User prepared library
10 nM of pooled library
15 ul
Please list your samples below (use additional sheets if needed):
Sample #
Sample name
Sample conc.
Volume (ul)
Organism
Core Use Only
1
2
3
4
5
6
7
8
MiSeq 1
LRI Genomics Core Facility
http://www.lerner.ccf.org/services/gc/
NE5-253, Phone: 216-444-0974
LERNER RESEARCH INSTITUTE
9
10
11
12
13
14
15
I, ______________________________ (PI’s name) declare that the samples submitted to the Core do not
contain an agents (biological or otherwise that can be harmful to those processing the samples
Name: _____________________________
Date: __________________________
Any related questions can be directed to: LRIgenomics@ccf.org
Date completed:
Performed by:
Charges:
FOR CORE
USE ONLY
MiSeq 2