NEXT GENERATION GENOMICS FACILITY
Service Requisition Form
Name of the PI:
Date:
Email:
Phone:
Institution Name:
Type of Organization:
Academic
Non-academic/Industry
Institution Address:
Billing Address:
The title of this project is ________________________________________________________
and is being funded by__________________________________________________________.
C-CAMP will provide us with Operator assisted service for the above project and help in
successful completion of the study. We will pay the user fee as per prevailing rates of CCAMP.
We agree to acknowledge the Genomics Facility at C-CAMP in any publications arising
out of the project.
Raw sequencing data will be deleted after 90 days of shipment of data. Please refer to our data
storage and access policy (http://ccamp.res.in/data-storage-and-access-policy-ngs).
Signature of research supervisor
Signature of service requestor
Centre for Cellular and Molecular Platform
NCBS-TIFR, GKVK Post, Bellary Road, Bengaluru 560065
Phone: 080-67185055
Email:services@ccamp.res.in
SAMPLE DETAILS
TYPE OF SEQUENCING REQUIRED
NUMBER OF
SAMPLES
NUMBER OF
LANES
Illumina Hiseq 1000
1
Lane number
(Please fill in the
lanes you would
like each sample
to be in)
Lane #
2
Lane #
3
Lane #
4
Lane #
5
Lane #
6
Lane #
7
Lane #
8
Lane #
9
Lane #
10
Lane #
11
Lane #
12
Lane #
13
Lane #
14
Lane #
15
Lane #
16
Lane #
S.No
Sample
Name
Organism and
Genome Size
(Gb)
Sample Type
(DNA
/RNA/small
RNA)
Method of
extraction
of
DNA/RNA
Concentration
of the Sample
ng/µl
Volume
(µl)
260/280 ratio
Single Read 50
cycles/
Paired End 100
cycles
Centre for Cellular and Molecular Platform
NCBS-TIFR, GKVK Post, Bellary Road, Bengaluru 560065
Phone: 080-67185055
Email:services@ccamp.res.in
Library Required (Whole
genome, Transcriptome,
Exome, small RNA,
Metagenomics,Mate-Pair,
Chip-Seq, Amplicon
Next Generation Sequencing Sample Submission Requirements
Whole Genome Sequencing (DNA): 3 µg of DNA per sample (~50 ng/ul concentration as measured by Qubit Fluorometer)
Transcriptome Sequencing (RNA): 3 µg of total RNA per sample (~100 ng/ul concentration as measured by Qubit Fluorometer)
Small RNA: 3 µg of total RNA per sample (~300 ng/ul concentration as measured by Qubit Fluorometer)
Mate Pair Library: 10 µg of DNA per sample (100 ng/ul concentration as measured by Qubit Fluorometer)
Exome: 10 µg of DNA per sample (~100 ng/ul concentration as measured by Qubit Fluorometer)
Chip-Seq: 30-50 ng of Chip DNA per sample (1 ng/ul concentration as measured by Qubit Fluorometer). The sample should be fragmented in the
range of 200 to 800 bases.
Shipment Details:
DNA and RNA samples should be shipped on DRY ICE. Please ship to address below: Addressed to - Dr.Malali Gowda
Special Instructions for DNA sample submission:
1. The DNA samples should be treated with RNAse A (eg: Invitrogen #12091-021) to get rid of RNA. The samples will not be processed if
there is RNA contamination.
2. The gel picture should be provided for the DNA samples; the volume loaded on the gel (eg.1 or 2 µl) should also be mentioned.
3. The concentrations and the 260/280 ratio should be measured in the nanodrop and the samples should be submitted in 1.5 mL microcentrifuge
tube and should be suspended in nuclease free water or elution buffer.
Special Instructions for RNA/small RNA sample submission:
1. The RNA samples should be treated with DNAse (we recommend DNA free TM from Ambion – #AM1906). The samples will not be
processed if they have a low RNA Integrity value(RIN), i.e < 8 after quality check by the Bioanalyzer. There will be an additional charge for
the Bioanalyzer incase the samples failed meeting the requirements.
2. The bioanalyzer profile should be provided for the RNA.
3. The concentrations and the 260/280 ratio should be measured in the nanodrop and the samples should be submitted in 1.5 mL microcentrifuge
tube and should be suspended in nuclease free water or elution buffer.
Centre for Cellular and Molecular Platform
NCBS-TIFR, GKVK Post, Bellary Road, Bengaluru 560065
Phone: 080-67185055
Email:services@ccamp.res.in