Grade 11 Biotechnology Cycles 1-20
Cycle
1-2
Cycle Overview
Lab safety - assessment
MSDS sheets - assessment
Lab contract - signage
Measurement techniques review – micropipettes and pipet-aids.
Equipment review: proper use of compound, dissecting and inverted microscopes,
spectrophotometers, autoclaves, still, dishwasher, pH meters, balances, photodocumentation
system, netbooks and iPADs.
Bacterial cultures, streak for isolation, strains and identification.
Absorption studies, serial dilution food coloring, bar graphs.
Weight determination, balances, repeatability, statistical analysis.
3-6
Photosynthesis – light and dark reactions, atoms, orbital, electrons, wavelengths. Photosystems,
electron transport chain, C4 and CAM plants, photorespiration.
Absorbance and transmission, chloroplast isolation, protein extraction from chloroplasts, gel
electrophoresis, determination of chlorophyll concentration, development of assay conditions
for O2 production, studies on the effect of wavelength on O2 production, pigment extractions
from leaves, comparison between pigments, absorption spectra and O2 production.
7-10
Tissue Culture – Aseptic technique and biosafety, review of eukaryotic and prokaryotic cells, cell
counting using a hemocytometer, dilutions, determination of PDL and PDT, cell culture
conditions, culture vessels, surfaces, media, contamination, bioethics-Henrietta Lacks, viruses
and cancer.
Cell counting practice with yeast cells (bioreactor) and hemocytometers, comparison of yeast
cell counts using hemocytometer and Bio-Rad counter, growth curves, lab notebook (ELN)
maintenance, practice aseptic techniques, initiate culture from frozen stock S2* cells, maintain
stocks, prepare cryopreserved stocks, growth curves, toxicity assays, precipitation and gel
electrophoresis of S3* proteins.
Written Exam: midterm shop assessment
11
Karyotyping, types of mutations, site-directed mutations using PCR, genes and heredity.
Solution preparation: %, molarity, w/v, and from stock solutions.
BSA protein assays, dilutions, Western blot – anti BSA antibody
12-14
Regulation of eukaryotic gene expression, bioinformatics,
Epigenetics, Product Development, upstream and downstream processing
The lifecycle of pharmaceutical products
Preparation of competent cells,
Transformation E.coli. JM109 with pAmy (encodes amylase) isolation of transformed bacteria,
DNA preparation, restriction analysis. Growth curve comparison between bacteria with and
without pAmy, bioreactor use, PAGE, Western blot for amylase production, anti-amylase
antibody, development of a purification protocol for amylase, Ammonium Sulfate cuts, dialysis,
ion exchange chromatography, Hydrophobic Interaction chromatography (HIC)
PAGE, Western blots
15-16
Animal regulations and oversight, The biotechnology workforce and history of biotechnology
Lagomorph species identification using DNA isolation and analysis: DNA isolation, use of
mitochondrial DNA for species identification, gel analysis of DNA fragment polymorphisms,
PCR, optimal enzyme conditions documentation
17
Product Quality – regulatory oversight
Review PCR and primer design
Identification of bacteria using PCR
18
Product Quality – regulatory oversight (cont.)
Blood and Blood Typing
19
Product Quality - Documentation
Bioreactors – growth conditions for S3 cells. Develop SOP
20
Written exam: final Shop assessment.
Make-up missing labs from field trips, snow days, etc.
Competency Assessments: lab safety, lab techniques, measurements, lab equipment,
documentation, pH meter, agarose gel preparation, solution prep, microbiology techniques,
protein purification techniques.
Lab inventory and ordering
Lab clean up