Mystery Meat Lab

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Mystery Meat lab Name_______________ Period_____

Make a standard graph on the back of this paper. Then determine the number of base pairs in each fragment in each lane.

Band

1

2

3

4

5

6

Standard

(mm) (Bp)

23,130

9,416

6,557

4,361

2,322

2,027

Mystery Meat

(mm) (Bp) (mm)

Beef

(Bp)

Raccoon

(mm) (Bp)

Squirrel

(mm) (Bp)

Skunk

(mm) (Bp) (mm)

Horse

(Bp)

1.

What is the function of restriction enzymes?

2.

What is the charge on the DNA Molecule?

3.

Which of the fragments of DNA will travel further in the gel?

4.

Why do DNA fragments move through the gel?

5.

In preparing your DNA samples for electrophoresis PCR was used. What is the purpose of using PCR?

6.

Where was the enzyme used in PCR found?

7.

Which sample has DNA fragments the same size as the Mystery Meat sample?

8.

What size are the fragments that match with the Mystery Meat?

Graph the Base Pairs (Bp) on the “y” axis and (mm) traveled on the “x” axis.

Base Pair

10

mm

15 mm

20 mm

25 mm

30

mm

35

mm

40 mm

45 mm mm 50

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