DNA Virtual Lab
Write answers on own notebook paper.
Go to http://learn.genetics.utah.edu. Then click “Virtual Labs” and you will look through each lab: DNA
Extraction, PCR, Gel Electrophoresis
1. Start with DNA Extraction Lab and tell the details of what happens in each stage of DNA
Extraction and which chemicals are used:
a. Collectionb. Bursting Cellsc. Separation of Materialsd. Isolation of DNA2. Next go to PCR and before you begin:
a. Define primer, DNA polymerase and nucleotide
b. Give the steps for completing PCR (don’t forget to tell what happens at each temperature
change 90, 50, 72)
c. How many copies are made in 30 cycles?
3. Finally, go to Gel Electrophoresis
a. List the 5 basic steps and give details on what you do at each step.
DNA Virtual Lab
Write answers on own notebook paper.
Go to http://learn.genetics.utah.edu. Then click “Virtual Labs” and you will look through each lab: DNA
Extraction, PCR, Gel Electrophoresis
1. Start with DNA Extraction Lab and tell the details of what happens in each stage of DNA
Extraction and which chemicals are used:
a. Collectionb. Bursting Cellsc. Separation of Materialsd. Isolation of DNA2. Next go to PCR and before you begin:
a. Define primer, DNA polymerase and nucleotide
b. Give the steps for completing PCR (don’t forget to tell what happens at each temperature
change 90, 50, 72)
c. How many copies are made in 30 cycles?
3. Finally, go to Gel Electrophoresis
a. List the 5 basic steps and give details on what you do at each step.