Lesson Plan: Bacterial Identification Using Technology
Objectives:
The students will be able to:
 distinguish between eukaryote and prokaryote DNA
 explain how to create more of a DNA sample so analysis of the DNA can take
place using PCR
 identify a specific bacterial pathogen which can cause food poisoning based
upon identification of a specific sequence of DNA
Activities:
Day 1:
Read through the case to determine what could have caused the illness
o Complete virtual lab on PCR from http://learn.genetics.utah.edu/content/labs/pcr/
 Set up the PCR tubes using proper techniques and protocol so that it can be
amplified

Day 2:
o Complete virtual lab on electrophoresis using the link
http://learn.genetics.utah.edu/content/labs/gel/
 Take sample and transfer contents to gel and run
 Stain the gel
Day 3:



Analyze the gel to determine if prokaryotes are present
Use nucleotide sequences to determine specific prokaryotes
Research the 6 bacteria to determine which is pathogenic and could have caused
the illness.
Homework:


Work on the questions in packet
Complete justification for selecting the one bacteria.