High Performance Liquid Chromatography Analysis of Phenolic Compounds,
Amino acids and Minerals in Prosopis
Thabit Al-Azzawe
Department of Medical Biochemistry College of Medicine, Al-Mustansiriya University
Email: halla94thabit@yahoo.com
A general procedure has been fixed poor analysis of phenolic compounds by reversed
phase-liquid chromatography with binary elution gradients of tetrahyrofuran (THF) and acidified
deionized water (2% acetic acids). The method was applied to the separation and estimation of
phenolic acids, tannin, phenolic aldehydes and coumarins from prosopis extract with detection
limit of 0.1 mg/ml. Free amino acids were separated by reverse phase precolumn derivatization,
using orthophathaldehyde (OPA) derivative. Essential trace element was analyzed in the extracts.