Real time PCR

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OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Infection with
Batrachochytrium dendrobatidis
Australian Animal Health Laboratory
CSIRO Livestock Industries
Private Bag 24, Geelong,
Victoria, 3220,
AUSTRALIA
Tel.:
(61 3) 5227 5000
Fax:
(61 3) 5227 5555
e-mail address:
website:
Name (including Title and
Position) of Head of Laboratory
(Responsible Official):
Name(including Title and
Position) of OIE Reference
Expert:
[email protected]
www.csiro.au
Dr Martyn Jeggo
Dr Alex Hyatt
Name (including Title and
Position) of writer of this report
(if different from above):
Annual reports of OIE Reference Centres, 2011
1
Infection with Batrachochytrium dendrobatidis
Part I: Summary of general activities related to the disease
1.
Test(s) in use/or available for the specified disease/topic at your laboratory
Regarding tests performed in 2011:
2.
Test
For
Specificity
Total
Real time PCR
DNA
Type
225
Immunohistochemistry
Antigen
Group
0
Production and distribution of diagnostic reagents

Reagents produced



DNA standards for real-time PCR
Antibodies for immunohistochemistry
Reagents supplied nationally
 DNA standards for real time PCR
 Antibodies for immunohistochemistry

Reagents supplied to other OIE Members
Type of reagent
Amount supplied nationally
(including for own use)
Amount supplied to
other countries
Standards for real time PCR
2
16
Antibodies for immunohistochemistry
2
0
Details of reagents sent both nationally and internally.
Date
Country
Volume
Item
22-Feb-11
USA
500 µl × 2
Bd std
03-Mar-11
USA
500 µl × 2
Bd std
03-Mar-11
USA
500 µl × 2
Bd std
15-Mar-11
USA
500 µl × 2
Bd std
15-Mar-11
Colombia
500 µl × 2
Bd std
11-Apr-11
USA
500 µl × 2
Bd std
26-May-11
Australia
500 µl × 1
Bd std
06-Jun-11
Australia
200 µl × 1
IPX
30-Aug-11
USA
500 µl × 2
Bd std
17-Oct-11
New Zealand
50 µl × 1
Bd std
Bd: Batrachochytrium dendrobatidis. Std: standard.
2
Annual reports of OIE Reference Centres, 2011
Infection with Batrachochytrium dendrobatidis
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
The Bd reference laboratory supplies reagents for real time PCR and immunocytochemisry. All reagents and
assays have been validated and standardised in different laboratories.
a) Establishment and maintenance of a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organisation of regular inter-laboratory
proficiency testing to ensure comparability of results
Only one designated laboratory exists.
b)
Organisation of inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results
Nil
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
See table above.
5.
Research and development of new procedures for diagnosis and control
No new tests under development.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
Best data can be found at [http://www.bd-maps.net/]. This is an international resource that shows distribution,
testing for Bd and species infected.
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned
AAHL’s diagnostic operations are conducted under NATA accreditation to ISO/IEC 17025:2005. In addition to its
NATA accreditation AAHL maintains certification to AS/NZS ISO 9001:2008 for the management of its Quality
Assurance System as well as AS/NZS ISO 14001:2004 for environmental management. AAHL is accredited to
ISO/IEC 17043 as an international proficiency testing provider for exotic disease agents.
8.
Provision of consultant expertise to OIE or to OIE Member Countries
Technical advice provided to various institutions on an ongoing basis
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
None in 2011. In previous years training given to scientists in USA, Australia, England and South America.
Annual reports of OIE Reference Centres, 2011
3
Infection with Batrachochytrium dendrobatidis
10. Provision of diagnostic testing facilities to other OIE Member Countries
Nil
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
Nil
12. Participation in international scientific collaborative studies
Nil
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)
Scientific publications in peer-reviewed journals
Hyatt, A.D., R. Speare, Cunningham AA. & Carey C. (2010). "Amphibian chytridiomycosis." Diseases of aquatic
organisms 92(2-3): 89-91.
Voyles, J., K. Richards-Hrdlicka, et al. (2010). "Batrachochytrium dendrobatidis: requirement for further isolate
collection and archiving." Diseases of aquatic organisms 92(2-3): 109-112.
Schloegel L.M.; Ferreira C.M.; James T.Y.; et al. (2010). The North American bullfrog as a reservoir for the
spread of Batrachochytrium dendrobatidis in Brazil. Animal Conservation Volume: 13 Supplement: 1 Pages: 5361.
_______________
4
Annual reports of OIE Reference Centres, 2011
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