OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Spherical baculovirosis
(Penaeus monodon-type baculovirus)
Aquaculture Pathology Laboratory
Department of Veterinary Science and Microbiology
University of Arizona
Building 90, Room 202 Pharmacy/Microbiology
Tucson, AZ 85721
UNITED STATES OF AMERICA
Tel.:
(1.520) 621.84.14
Fax:
(1.520) 621.48.99
e-mail address:
website:
ldvl@u.arizona.edu
http://microvet.arizona.edu/research/aquapath/index.htm
Name (including Title and
Position) of Head of Laboratory
(Responsible Official):
Dr Donald V. Lightner
Professor
Name(including Title and
Position) of OIE Reference
Expert:
Dr Donald V. Lightner
Professor
Name (including Title and
Position) of writer of this report
(if different from above):
Annual reports of OIE Reference Centres, 2011
Dr Donald V. Lightner
&
Linda Nunan, Assistant Staff Scientist
1
Spherical baculovirosis (Penaeus monodon-type baculovirus)
Part I: Summary of general activities related to the disease
1.
2.
Test(s) in use/or available for the specified disease/topic at your laboratory
Test
For
Specificity
Total
PCR
Surveillance & SPF
verification
All known MB viral
genotypes
335
Histology
Initial diagnosis &
surveillance
Lesions
ISH
Confirmatory diagnosis
All known MB viral
genotypes
0
Real-time PCR
Confirmatory diagnosis &
quantification of viral load
All known MB viral
genotypes
0
2419
Production and distribution of diagnostic reagents
Date
Country
Material provided
Quantity sent
04/20/2011
Madagascar
MBV primers
2 tubes
06/11/2011
India
MBV clone
1 vial
11/23/2011
Saudi Arabia
MBV clone
1 vial
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
a)
Establishment and maintenance of a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organisation of regular inter-laboratory
proficiency testing to ensure comparability of results
None
b)
Organisation of inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results
None
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
See section Part I-2:

2
Plasmid DNA for use as positive control in PCR reactions
Annual reports of OIE Reference Centres, 2011
Spherical baculovirosis (Penaeus monodon-type baculovirus)
5.
Research and development of new procedures for diagnosis and control
Duplex real-time PCR for detection and quantification of monodon baculovirus (MBV) and hepatopancreatic
parvovirus (HPV) in Penaeus monodon:
Tang K.F.J. & D.V. Lightner. In press. Duplex real-time PCR for detection and quantification of monodon
baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon. Diseases of Aquatic Organisms.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
None
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned


8.
Quality assurance of sample material provided is achieved by pre-testing the sample material for the pathogen
of interest, by PCR, prior to shipment.
Biosafety and biosecurity is achieved by following federal regulations for disposal of infectious materials and
through the inland location of the laboratory, as no marine pathogens would be able to survive in the natural
environment of the Sonora Desert.
Provision of consultant expertise to OIE or to OIE Member Countries
Chapters for the OIE Aquatic Animal Health Code – assisted in preparation
Lightner DV. 2011. Aquatic Animal Health Code, 14th Edition. World Organization for Animal Health (Office
International des Epizooties = OIE), Paris:
- Infectious hypodermal and haematopoietic necrosis, Chapter 9.2.
- Infectious myonecrosis, Chapter 9.3.
- Necrotizing Hepatopancreatitis, Chapter 9.4.
- Taura syndrome, Chapter 9.5.
- White spot disease, Chapter 9.6.
- White tail disease, Chapter 9.7.
- Yellow head disease, Chapter 9.8.
Chapters for the OIE Manual of Diagnostic Tests for Aquatic Animals
Lightner DV. In press (2012). Diagnostic Manual for Aquatic Animal Diseases, 7th Edition. World Organization
for Animal Health (Office International des Epizooties = OIE), Paris:
- Disinfection of Crustacean Farms, Section C of Methods for Disinfection of Aquaculture Establishments.
- Diseases of Crustaceans, General Information.
- Infectious Hypodermal and Hematopoietic Necrosis.
- Infectious Myonecrosis.
- Taura Syndrome.
- Tetrahedral Baculoviruosis (Baculovirus penaei).
- Spherical Baculovirosis (Penaeus monodon-type baculovirus).
Advisor to the Aquatic Animal Health Standards Commission, Office International Des Epizooties, Paris. Term
2009-2011, Advisor (~10 days/year). I was appointed to a third 3-year term by the OIE Central Bureau, beginning in
May 2009, on the OIE Aquatic Animal Health Standards Commission (AAHSC) which meets 2 times per year to
consider new diagnostic methods for OIE listed and notifiable aquatic animal diseases; to consider changes to the
OIE Aquatic Animal Code; and to make recommendations to the Director General and to the OIE World Assembly
of Member Country Delegates. In 2011, I participated in the AAHSC meeting of October 3-7 at the OIE
Headquarters in Paris.
Annual reports of OIE Reference Centres, 2011
3
Spherical baculovirosis (Penaeus monodon-type baculovirus)
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
• Hosted Borlaug scholar Mr Bambang Hanggono, of the Indonesian Government’s Situbondo Laboratory
Mr Hanggono visited the Aquaculture Pathology Laboratory for 12 weeks (17 September to 3 December, 2011).
Mr Hanggono’s training emphasized histopathology and molecular diagnostic tests for major shrimp diseases
including Tetrahedral baculovirosis virus.
10. Provision of diagnostic testing facilities to other OIE Member Countries
None.
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
None.
12. Participation in international scientific collaborative studies
None
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Presentations at international conferences and meetings
Invited speaker OECD Symposium, Halifax, Canada. Given by OECD Co-operative Research Program, Paris,
8/7/2011.
Invited keynote speaker, Honduras. Given by V Simposio Nicovita, Honduras, 11/24/2011. (100%).
Invited keynote speaker, Ecuador. Given by XIII Congreso Ecuatoriano de Acuicultura and Aquaexpo,
10/20/2011.
Invited speaker - OIE Global Conference on Aqiuatic Animal Health, Panama. Given by OIE , 6/29/2011.
Invited keynote speaker, Panama City, Panama. Given by El Comite Organizador del X Simposio International de
Camaron de Cultivo y Exhibicion Comercial, 5/13/2011.

Scientific publications in peer-reviewed journals
Lightner D.V. and R.M. Redman. 2010. The global status of significant infectious diseases of farmed shrimp.
Asian Fisheries Society Journal 23(4): 383-426.
Lightner D.V. 2011. Virus diseases of farmed shrimp in the Western Hemisphere (the Americas): a review.
Journal of Invertebrate Pathology 106: 110-130.
Lightner, D.V. 2011. Status of shrimp diseases and advances in shrimp health management. pp. 121-134, in M.G.
Bondad-Reantaso, J.B. Jones, F. Corsin and T. Aoki (editors), Diseases in Asian Aquaculture VII. Fish Health
Section, Asian Fisheries Society, Selangor, Malaysia. 385 p.
Lightner, D.V. and R.M. Redman. "In press". Development of specific pathogen-free (SPF) shrimp stocks and
their application to sustainable shrimp farming. Woodhead Publishing, LTD., Cambridge. UK.
Tang K.F.J., and D.V. Lightner. In press. Duplex real-time PCR for detection and quantification of monodon
baculovirus (MBV) and hepatopancreatic parvovirus (HPV) in Penaeus monodon. Diseases of Aquatic Organisms.
4
Annual reports of OIE Reference Centres, 2011
Spherical baculovirosis (Penaeus monodon-type baculovirus)

Other communications
Lightner, D.V. 2011. Enfermedades virales en camarones cultivados en el hemisferio oeste: una reseña. .
Enfermedades virales en camarones cultivados en el hemisferio oeste: una reseña. Panorama Acuícola 16(4):
8-20.
Arce, S.M., S.M. Moss and D.V. Lightner. 2011. Biosecurity principles for sustainable production using SPF
shrimp. Global Aquaculture Advocate. May/June 2011, 14-16.
_______________
Annual reports of OIE Reference Centres, 2011
5