OIE Reference Laboratory Reports
Activities in 2012
Name of disease (or topic) for which you
are a designated OIE Reference Laboratory:
Spring Viraemia of Carp
Address of laboratory
1011 Fuqiang Road,
Shenzhen Guangdong province 518045
THE PEOPLE’S REPUBLIC OF CHINA
Tel.:
+86-755 25.58.84.10
Fax:
+86-755 25.58.86.30
e-mail address:
liuhong@szciq.gov.cn
website:
http://www.szciq.gov.cn/dzzx/
Name (including Title) of Head of
Laboratory (Responsible Official):
Director Tikang Lu
Name (including Title and Position) of OIE
Reference Expert:
Dr Hong LIU
Date of submission to the OIE
Jan. 20th, 2013
Annual reports of OIE Reference Centres, 2012
1
OIE RL for « Spring Viraemia of Carp » – « Hong LIU » – « China »
Test recommended by the OIE
Total number of test performed last year
Indirect diagnostic tests
Nationally
Internationally
Direct diagnostic tests
Nationally
Internationally
Cell Culture (EPC, CO, FHM)
2346
0
RT-PCR gene G
2346
0
The laboratory produced:
2.

Monoclonal antibodies against SVCV selected with good affinity which will be used in the
ELISA tests or Colloidal gold Strip;

Expressing the complete glycoprotein of SVCV in the baculovirus expressing system and insect
cell line;

Selecting 8 antibodies against purified SVCV virions with the phage display libraries which will
be used in the ELISA tests or Colloidal gold Strip;

RT-PCR kits, real-time PCR kits and LAMP kits for SVCV were prepared.

Validation of the susceptibility of grass carp ovary cell line (CO) for SVCV.
Did your laboratory produce or store imported standard reference reagents officially recognised by
the OIE or other international bodies?
Yes
3.
No
Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes
4.
No
Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
Yes
5.
No
Did your laboratory produce vaccines?
Yes
6.
Did your laboratory supply vaccines to OIE Member Countries?
Yes
7.
No
Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen
or disease?
Yes
2
No
Did your laboratory develop new diagnostic methods validated according to OIE Standards for the
designated pathogen or disease?
Yes
8.
No
No
Annual reports of OIE Reference Centres, 2012
OIE RL for « Spring Viraemia of Carp » – « Hong LIU » – « China »
9.
Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes
10.
No
Did your laboratory provide expert advice in technical consultancies on the request of an OIE
Member Country?
Yes
11.
No
Did your laboratory participate in international scientific studies in collaboration with OIE Member
Countries other than the own?
Yes
12.
No
Did your Laboratory collect epizootiological data relevant to international disease control?
Yes
13.
No
Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes
14.
No
What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a) .........................................................................................Articles published in peer-reviewed
journals: ........................................................................... 1
YANG Zhen-hui, GE Jun-qing, LIU Hong, et al., 2012. Prokaryotic expression of Spring viremia of carp virus
matrix protein and preparation of polyclonal antibody. J. Fujian Agriculture and Forestry University, 40(6):614-618
b) ........................................................................ International conferences:
c) ........................................................................ National conferences:
0
0
d) ........................................................................ Other: 0 internet
15.
Did your laboratory provide scientific and technical training to laboratory personnel from other OIE
Member Countries?
Yes
16.
No
Does your laboratory have a Quality Management System certified according to an International
Standard?
Yes
No
Quality management system adopted
ISO 17025
17.
Is your laboratory accredited by an international accreditation body?
Yes
No
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease
concerned?
Annual reports of OIE Reference Laboratories, 2012
3
OIE RL for « Spring Viraemia of Carp » – « Hong LIU » – « China »
Yes
19.
No
Did your laboratory organise scientific meetings on behalf of the OIE?
Yes
20.
No
Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes
21.
No
Did your laboratory exchange information with other OIE Reference Laboratories designated for the
same pathogen or disease?
Yes
22.
No
Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated
for the same pathogen or disease by organising or participating in proficiency tests?
Yes
23.
No
Did your laboratory collaborate with other OIE Reference Laboratories for the same disease
on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes
24.
No
Did your laboratory organise or participate in inter-laboratory proficiency tests with
laboratories other than OIE Reference Laboratories for the same disease?
Yes
No
Purpose for inter-laboratory test comparisons1
No. participating
laboratories
Participating OIE Member
Countries
Harmonising existing test methods (specify: RTPCR for SVCV detection)
5
4 laboratories in P. R. China and 1
in Hongkong
Harmonising existing test methods (specify: cell
line susceptibility and RT-PCR for SVCV detecion)
Participating the
proficiency tests
organized by
European Fish
Reference
Laboratory
P. R. China and the member
countries of EU
Evaluating new test methods (specify : Protocol
for Real-time RT-PCR test for SVCV detection )
2
P. R. China and Hongkong
25.
Did your laboratory place expert consultants at the disposal of the OIE?
Yes
1
4
No_____
See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Annual reports of OIE Reference Centres, 2012