Positive control for PCR

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OIE Reference Laboratory Reports
Activities in 2011
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Oncorhynchus masou virus disease
Laboratory of Marine Biotechnology and Microbiology
Faculty of Fisheries Sciences
Hokkaido University
Hakodate,
Hokkaido 041-8611,
JAPAN
Tel.:
+81-138-40-8810
Fax:
+81-138-40-8810
e-mail address:
[email protected]
website:
Name (including Title and
Position) of Head of Laboratory
(Responsible Official):
Professor Mamoru YOSHIMIZU
Name(including Title and
Position) of OIE Reference
Expert:
Professor Mamoru YOSHIMIZU
Name (including Title and
Position) of writer of this report
(if different from above):
Annual reports of OIE Reference Centres, 2011
Professor Mamoru YOSHIMIZU
and Associate Professor Hisae KASAI
1
Oncorhynchus masou virus disease
Part I: Summary of general activities related to the disease
1.
2.
Test(s) in use/or available for the specified disease/topic at your laboratory
Test
For
Specificity
RTG-2 cell culture
Virus isolation
Total
540
Production and distribution of diagnostic reagents
The laboratory produced:
・Polyclonal antibody to identify OMV.
・Details of PCR primer sequences and protocols for detection and confirmation of OMV.
・Extracted DNA of culture OMV as PCR template.
One ampoule of freeze dried antibody was supplied for representative Prefectural Fisheries Experimental Station
and supplied for member stations.
Type of reagent
Amount supplied nationally
(including for own use)
Amount supplied to other
countries
Control positive serum
10 ml
0 ml
Immunofluorescent conjugate
1 ml
0 ml
Antigens for ELISA test
10 plates
0 plate
Positive control for PCR
requested
20 pieces
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
a)
Establishment and maintenance of a network with other OIE Reference Laboratories
designated for the same pathogen or disease and organisation of regular inter-laboratory
proficiency testing to ensure comparability of results
None.
b)
Organisation of inter-laboratory proficiency testing with laboratories other than OIE
Reference Laboratories for the same pathogens and diseases to ensure equivalence of
results
None.
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
Preparation for the distribution of antibody against OMV, details of PCR primer sequences and protocols for
detection and confirmation of OMV, extracted DNA of culture OMV as PCR template. Details of PCR primer
2
Annual reports of OIE Reference Centres, 2011
Oncorhynchus masou virus disease
sequences and protocols for detection and confirmation of OMV, and extracted DNA of culture OMV were
supplied for international reference standard.
5.
Research and development of new procedures for diagnosis and control
The following research; establishment of the in situ hybridization method is being carried out.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
Conducted the cooperative studies with personnel from laboratories in the USA, Korea, Taiwan, Thailand, China
and Holland.
 Epidemiological surveys
purpose: epizootiological surveys of salmonid virus including OMV
participants: Faculty of Fisheries Sciences, Hokkaido University
time frame: October 2011-December 2011
outputs and follow up plans: All 540 of chum salmon are negative. the yearly epizootiological tests continues.
 Scientific projects: wholel genome analysis of OMV
Purpose: Comparison of isolates using whole genome analysis
Participant: Faculty of Fisheries Sciences, Hokkaido University
time frame: 2010-2011
outputs and follow up plans: most of the genome from the three isolates including type strain OO-7812 was
determined and ORFs were analyzed (Preparing for submission) . Sequence of isolates from
salmonids will be compared.
7.
Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the
pathogen and the disease concerned
Introduce the oncogenic viruses in “Fish Disease & Disorders”, Volume 2, Chapter 7.
8.
Provision of consultant expertise to OIE or to OIE Member Countries
 Writing or reviewing texts for the OIE Aquatic or Terrestrial Manuals or Codes
 Attending OIE Commissions and ad hoc groups to provide expert advice
9.
Provision of scientific and technical training to personnel from other OIE Member Countries
No requests for diagnosis were received from other Member Countries.
10. Provision of diagnostic testing facilities to other OIE Member Countries
Each 10 pieces of PCR positive control were sent for two countries
11. Organisation of international scientific meetings on behalf of OIE or other international bodies
Advanced Program to Foster Young and Female Researchers from Southeast Asia in Sustainable Fisheries
Sciences.
Annual reports of OIE Reference Centres, 2011
3
Oncorhynchus masou virus disease
12. Participation in international scientific collaborative studies
None.
13. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Presentations at international conferences and meetings
Yoshimizu, M. and H. Kasai: Control strategy for viral diseases of salmonid fish and flounders at hatchery or seed
production facility, and net pen or tank in Japan. 3rd International Symposium on Cage Aquaculture in Asia,
Kuala Lumpur, Malaysia, November 16-19, 2011.

Scientific publications in peer-reviewed journals
Yoshimizu, M. and H. Kasai (2012): Mini review: Control strategy for viral diseases of salmonid fish and
flounders at hatchery or seed production facility, and net pen or tank in Japan. Journal of Asian Fisheries Societies,
in print.
_______________
4
Annual reports of OIE Reference Centres, 2011
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