OIE Reference Laboratory Reports Activities in 2011 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Address of laboratory: Oncorhynchus masou virus disease Laboratory of Marine Biotechnology and Microbiology Faculty of Fisheries Sciences Hokkaido University Hakodate, Hokkaido 041-8611, JAPAN Tel.: +81-138-40-8810 Fax: +81-138-40-8810 e-mail address: yosimizu@fish.hokudai.ac.jp website: Name (including Title and Position) of Head of Laboratory (Responsible Official): Professor Mamoru YOSHIMIZU Name(including Title and Position) of OIE Reference Expert: Professor Mamoru YOSHIMIZU Name (including Title and Position) of writer of this report (if different from above): Annual reports of OIE Reference Centres, 2011 Professor Mamoru YOSHIMIZU and Associate Professor Hisae KASAI 1 Oncorhynchus masou virus disease Part I: Summary of general activities related to the disease 1. 2. Test(s) in use/or available for the specified disease/topic at your laboratory Test For Specificity RTG-2 cell culture Virus isolation Total 540 Production and distribution of diagnostic reagents The laboratory produced: ・Polyclonal antibody to identify OMV. ・Details of PCR primer sequences and protocols for detection and confirmation of OMV. ・Extracted DNA of culture OMV as PCR template. One ampoule of freeze dried antibody was supplied for representative Prefectural Fisheries Experimental Station and supplied for member stations. Type of reagent Amount supplied nationally (including for own use) Amount supplied to other countries Control positive serum 10 ml 0 ml Immunofluorescent conjugate 1 ml 0 ml Antigens for ELISA test 10 plates 0 plate Positive control for PCR requested 20 pieces Part II: Activities specifically related to the mandate of OIE Reference Laboratories 3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines a) Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results None. b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results None. 4. Preparation and supply of international reference standards for diagnostic tests or vaccines Preparation for the distribution of antibody against OMV, details of PCR primer sequences and protocols for detection and confirmation of OMV, extracted DNA of culture OMV as PCR template. Details of PCR primer 2 Annual reports of OIE Reference Centres, 2011 Oncorhynchus masou virus disease sequences and protocols for detection and confirmation of OMV, and extracted DNA of culture OMV were supplied for international reference standard. 5. Research and development of new procedures for diagnosis and control The following research; establishment of the in situ hybridization method is being carried out. 6. Collection, analysis and dissemination of epizootiological data relevant to international disease control Conducted the cooperative studies with personnel from laboratories in the USA, Korea, Taiwan, Thailand, China and Holland. Epidemiological surveys purpose: epizootiological surveys of salmonid virus including OMV participants: Faculty of Fisheries Sciences, Hokkaido University time frame: October 2011-December 2011 outputs and follow up plans: All 540 of chum salmon are negative. the yearly epizootiological tests continues. Scientific projects: wholel genome analysis of OMV Purpose: Comparison of isolates using whole genome analysis Participant: Faculty of Fisheries Sciences, Hokkaido University time frame: 2010-2011 outputs and follow up plans: most of the genome from the three isolates including type strain OO-7812 was determined and ORFs were analyzed (Preparing for submission) . Sequence of isolates from salmonids will be compared. 7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned Introduce the oncogenic viruses in “Fish Disease & Disorders”, Volume 2, Chapter 7. 8. Provision of consultant expertise to OIE or to OIE Member Countries Writing or reviewing texts for the OIE Aquatic or Terrestrial Manuals or Codes Attending OIE Commissions and ad hoc groups to provide expert advice 9. Provision of scientific and technical training to personnel from other OIE Member Countries No requests for diagnosis were received from other Member Countries. 10. Provision of diagnostic testing facilities to other OIE Member Countries Each 10 pieces of PCR positive control were sent for two countries 11. Organisation of international scientific meetings on behalf of OIE or other international bodies Advanced Program to Foster Young and Female Researchers from Southeast Asia in Sustainable Fisheries Sciences. Annual reports of OIE Reference Centres, 2011 3 Oncorhynchus masou virus disease 12. Participation in international scientific collaborative studies None. 13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences) Presentations at international conferences and meetings Yoshimizu, M. and H. Kasai: Control strategy for viral diseases of salmonid fish and flounders at hatchery or seed production facility, and net pen or tank in Japan. 3rd International Symposium on Cage Aquaculture in Asia, Kuala Lumpur, Malaysia, November 16-19, 2011. Scientific publications in peer-reviewed journals Yoshimizu, M. and H. Kasai (2012): Mini review: Control strategy for viral diseases of salmonid fish and flounders at hatchery or seed production facility, and net pen or tank in Japan. Journal of Asian Fisheries Societies, in print. _______________ 4 Annual reports of OIE Reference Centres, 2011