OIE Reference Laboratory Reports Activities in 2012 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Address of laboratory Newcastle Disease Australian Animal Health Laboratory CSIRO Livestock Industries Private Bag 24 Geelong VIC 3220 AUSTRALIA Tel.: +61 3 5227 5000 Fax: +61 3 5227 5555 e-mail address: peter.daniels@csiro.au website: www.csiro.au Name (including Title) of Head of Laboratory (Responsible Official): Dr Kurt Zuelke Name (including Title and Position) of OIE Reference Expert: Paul Selleck (Retired) Date of submission to the OIE 23 January 2013 Annual reports of OIE Reference Centres, 2012 1 OIE RL for « Newcastle Disease » – « Paul Selleck » – « Australia » ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards Test recommended by the OIE Total number of test performed last year Indirect diagnostic tests Nationally Internationally C-ELISA NDV Group 0 Haemagglutination inhibition (HI) test 2108 487 APMV2 0 Direct diagnostic tests Nationally Internationally Taqman real-time PCR for NDV 873 60 PCR and sequencing F gene cleavage site (Pathotyping) 34 60 PCR and sequencing HA and F gene 34 60 Embryonated chicken egg culture 379 60 Immunohistochemistry for NDV 10 4 Virus Typing by HI 25 0 APMV1 (NDV) Haemagglutination inhibition (HI) test APMV2-9 ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies? Yes 3. No Did your laboratory supply standard reference reagents to OIE Member Countries? Yes Type of reagent available Related diagnostic test Control positive PCR reagent Real time PCR No Produced/ stored Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) Produced ?? 4.5 ml (NDV/chicken/Mya nmar/2012) And control negative allantoic fluid Control positive antiserum And control negative chicken antiserum 2 4.5 ml HI serology Produced 2.7 ml 2.7 ml Name of recipient OIE Member Countries and of institutions Cambodia, China, Indonesia, Lao PDR, Myanmar, Malaysia, Philippines, Thailand, Vietnam Cambodia, China, Indonesia, Lao PDR, Myanmar, Malaysia, Philippines, Thailand, Vietnam Annual reports of OIE Reference Centres, 2012 OIE RL for « Newcastle Disease » – « Paul Selleck » – « Australia » 4. Type of reagent available Related diagnostic test Produced/ stored Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) Name of recipient OIE Member Countries and of institutions Network Quality Control (low positive control) NDV qPCR produced 35 ml - - Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents? Yes 5. No Did your laboratory produce vaccines? Yes 6. No Did your laboratory supply vaccines to OIE Member Countries? Yes No ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 7. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? x Yes 8. No Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Yes No Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) Real-time PCR for detection of avian paramyxovirus 1 (APMV1) of pigeon origin (PPMV-1) Validation data maintained in the AAHL Quality Assurance System shows a test with increased sensitivity over the NDV qPCR for the detection of PPMV-1 agent in swabs ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 9. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Yes No Name of OIE Member Country seeking assistance Date (dd/mm) No. samples received for provision of diagnostic support (i.e. from surveillance campaign) No. samples received for provision of confirmatory diagnoses Nepal 5 April 0 10 Myanmar 4 May 0 10 Annual reports of OIE Reference Laboratories, 2012 3 OIE RL for « Newcastle Disease » – « Paul Selleck » – « Australia » Indonesia 17 May 0 36 Papua New Guinea 7 March, 30 April, 4 June, 3 August, 23 November 434 0 East Timor 19 November 53 0 0 4 New Zealand 10. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes Name of the OIE Member Country receiving a technical consultancy Pakistan No Purpose How the advice was provided Establish a Crisis Management Centre in Animal Health, focusing on NDV, July 2012 Technical assistance mission ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 11. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Yes No ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 12. Did your Laboratory collect epizootiological data relevant to international disease control? Yes 13. No Did your laboratory disseminate epizootiological data that had been processed and analysed? Yes 14. No What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: ................. 1 b) International conferences: .............................................. 1 c) National conferences: ...................................................... 1 d) Other: ............................................................................... ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests of vaccines as OIE Standards 15. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? Yes 4 No Annual reports of OIE Reference Centres, 2012 OIE RL for « Newcastle Disease » – « Paul Selleck » – « Australia » a) Technical visits: ................................................................ 6 b) Seminars: ......................................................................... NA c) Hands-on training courses: .............................................. 2 d) Internships (>1 month): ................................................... NA Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training No. participants from the corresponding country a Myanmar, Cambodia, Malaysia, Vietnam, Philippines and Thailand 20 a Malaysia 4 a Malaysia 6 a Malaysia 10 a Malaysia 4 c In Thailand, for trainees from Myanmar, Cambodia, Malaysia, Vietnam, Philippines and Thailand 16 c Malaysia 8 ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 16. Does your laboratory have a Quality Management System certified according to an International Standard? Yes No AAHL maintains certification to AS/NZS ISO 9001:2008 for the management of its Quality Assurance System as well as AS/NZS ISO 14001:2004 for environmental management. 17. Is your laboratory accredited by an international accreditation body? Yes No AAHL’s diagnostic operations are conducted under NATA accreditation to ISO/IEC 17025:2005. AAHL is also accredited to ISO/IEC 17043:2010 as an international proficiency testing provider for exotic disease agents. Test for which your laboratory is accredited AAHL’s scope of accreditation includes th efolowing classes of tests : 20.10 Microbiology For companion animals, production animals, production avian species, aquatic animals, equine species and avian species Accreditation body NATA, affiliated with ILAC 20.11 Bacteriology .01 Diagnostic bacteriology - incorporating identification by simple microscopy, cultural methods of detection and identification of organisms .03 Immunological methods of antigen detection 20.13 Other Microorganisms (including mycoplasma, rickettsia, algae) .01 Diagnostic microbiology - incorporating identification by simple microscopy, cultural methods of detection and identification of organisms, including inocuity testing Annual reports of OIE Reference Laboratories, 2012 5 OIE RL for « Newcastle Disease » – « Paul Selleck » – « Australia » 20.14 Virology .01 Diagnostic virology - non-cultural (immunological) methods of detection .02 Diagnostic virology - cultural methods of detection and identification of organisms, including inocuity testing .05 Quantitative procedures 20.15 Prions .01 Histological identification of prion disease lesions .02 Detection of prion protein by immunological methods (including ELISA, Western Blots, immunohistochemistry) .04 Detection of prion protein by bioassay 20.25 Serology of Infection For companion animals, production animals, production avian species, equine species and avian species .01 Agar gel immunodiffusion tests .02 Complement fixation tests .03 Enzyme linked immunosorbent assays .04 Haemagglutination inhibition .05 Indirect fluorescent antibody tests .06 Microscopic agglutination tests .08 Serum agglutination tests .09 Serum neutralisation tests .10 Latex agglutination tests .99 Other : Testing for rabies and rabies related lyssaviruses on human specimens 20.50 Anatomical Pathology For companion animals, production animals, production avian species, laboratory animals, zoo animals, wildlife, aquatic animals, equine species and avian species 20.52 Histopathology .01 Processing of fixed specimens for histology .04 Immunohistochemistry .05 Histological interpretation 20.53 Electron Microscopy .01 Transmission electron microscopy .02 Scanning electron microscopy .04 Immunohistochemistry electron microscopy 20.54 Necropsy 20.80 Molecular Diagnostics .01 Identification by extraction and amplification .02 Sequencing .03 Genotyping .99 Other : Testing for rabies and rabies related lyssaviruses on human specimens by molecular techniques 18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1) Yes No ToR: To organise and participate in scientific meetings on behalf of the OIE 6 Annual reports of OIE Reference Centres, 2012 OIE RL for « Newcastle Disease » – « Paul Selleck » – « Australia » 19. Did your laboratory organise scientific meetings on behalf of the OIE? Yes 20. No Did your laboratory participate in scientific meetings on behalf of the OIE? Yes Title of event The 7th Asia-Pacific Biosafety Association Scientific Conference, Moving Towards One World-One Health, No Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented 04/12 Bali, Indonesia. Speaker Emerging and Reemerging Disease Control. Facing New Challenges: Emerging Pathogens & Disease Surveillance ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 21. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? Yes 22. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Yes 23. No No Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Yes No ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results. 24. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Yes Annual reports of OIE Reference Laboratories, 2012 No 7 OIE RL for « Newcastle Disease » – « Paul Selleck » – « Australia » Purpose for inter-laboratory test comparisons1 No. participating laboratories Participating OIE Member Countries Test laboratory sensitivity and specificity for Newcastle disease viruses by real time PCR 7 Australia (State government laboratories in the national laboratory network) Test laboratory sensitivity and specificity for Newcastle disease viruses by real time PCR 10 Cambodia, China, Indonesia, Lao PDR, Myanmar, Malaysia, Philippines, Thailand, Vietnam Test laboratory sensitivity and specificity for detection of antibody to Newcastle disease virus by the haemagglutination inhibition (HI) test 11 Cambodia, China, Indonesia, Lao PDR, Myanmar, Malaysia, Philippines, Thailand, Vietnam, and Timor Leste ToR: To place expert consultants at the disposal of the OIE 25. Did your laboratory place expert consultants at the disposal of the OIE? Yes No Kind of consultancy Location Subject (facultative ) A scoping mission Bangladesh To create a workshop on Laboratory Networking and Proficiency Testing for priority HPEDs in SAARC countries January 2012 OIE Training Workshop Thailand For OIE National Focal Points on Laboratories, August 2012 Meeting participation Vietnam The Fourth Laboratory Directors Forum Meeting (SE Asian region), October 2012 Meeting participation Vienna Expert consultation for establishing and maintaining core facilities for molecular test diagnosis in veterinary laboratories of developing countries with limited resources, October 2012. Ad hoc group participation Paris Biosafety and Biosecurity in Veterinary Laboratories, February and July, 2012 Ad hoc group participation Paris New Approaches to Diagnosis: Applied Genomics, December 2012 Member of the OIE Biological Standards Commission Paris Particpated in the BSC meeting September 2012 Publications Conference Papers Shan, S. (2012). Avian paramyxovirus serotype 1 (APMV-1) in Australia. Paper presented at the The Asian-Pacific Regional Workshop on Newcastle disease in China, Qingdao, China. Shan, s. (2012). Biological and molecular characterization of an avian paramyxovirus Type 1 isolated from domestic pigeons in Victoria, 2011. Paper presented at the AAVLD, Melbourne, Vic. Journal Article Serrao, E., Meers, J., Pym, R., Copland, R., Eagles, D., & Henning, J. (2012). Prevalence and incidence of Newcastle disease and prevalence of Avian Influenza infection of scavenging village chickens in Timor-Leste. Preventive Veterinary Medicine, 104(3-4), 301-308. doi: 10.1016/j.prevetmed.2011.12.018 1 8 See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3 Annual reports of OIE Reference Centres, 2012