Capillary Zone Electrophoresis
Power Supply:
Solvent Reservoir:
Capillary Tube:
High Voltage, enables
the use of positive and
negative charge to
separate the analyte.
Positively charged solvent.
Bridges positive and negative
solvent reservoir, separation
occurs in tube.
Display:
Solvent Reservoir:
Detector:
Read out of data from
detector
Negatively charged solvent.
In line with the capillary tube
to analyze analyte. (UV-Vis)
(Inside safety glass [Plexiglas]
box)
Vendors:
Agilent Technologies
ATTO Corporation
Biomidi
Capital HPLC Limited
CETAC Technologies, Inc.
Computer Power Supply, CPS
Helena BioSciences Europe
UVP, Inc.
Vilber Lourmat
WynSep
Walbroehl, Y.; Jorgenson, J. W. Capillary zone electrophoresis of neutral organic molecules by
solvophobic association with tetraalkylammonium ion. Anal. Chem., 1986, 58 (2), pp 479–481.
Yu, M.; Dovichi, N. J. Attomole amino acid determination by capillary zone electrophoresis with
thermooptical absorbance detection. Anal. Chem., 1989, 61 (1), pp 37–40.
Higashijima, T.; Fuchigami, T.; Imasaka, T.; Ishibashi, N. Determination of amino acids by capillary zone
electrophoresis based on semiconductor laser fluorescence detection. Anal. Chem., 1992, 64 (7), pp
711–714.
Uses for analysis:
Separating nucleic acids
Separating proteins