BSCS Packet #6 – Cells - Internal Environment, part 2 (Unit 4) 2014-2015 This Activity Packet belongs to: __________________________ At the end of the unit you will turn in this packet. You should expect a variety of quizzes: announced, unannounced, open-notes and closed-notes. 5E Model Packet Due Date for Activity page Completion Engage Lab 4-3: Animal vs Plant Microscope Lab Explore Explain Elaborate Journal 4-4: Cells Alive Online Activity – Animal vs Plant; Prokaryote vs Eukaryote Journal 4-5: Prokaryote and Eukaryote Cells J 4-6 Doctor Diagnosis Cells reading guide part 2 (Dragonfly Textbook) Evaluate Unit 4 Review Guide for Part 2 If this packet is LOST, please: drop it off at the BHS Science Dept. (rm 365) OR drop it off in __________ classroom (rm _____) OR call the Science Dept. at (617) 713-5365. 0 Lab 4-3: Animal vs Plant Microscope Lab Each group will create a plant cells slide and look at two different prepared animal cell/tissue slides. PART I: ANIMAL CELL OBSERVATIONS 1. Choose two different slides of human tissue. 2. Using a pencil, sketch what you observe. Label your drawing with the magnification used. II. Type of Cell/Tissue: ________________ Total Magnification: ____ X Type of Cell/Tissue: ________________ Total Magnification: ____ X PART II: PLANT CELL OBSERVATIONS (Elodea cells) 1. Obtain a piece of Elodea leaf, and place it on a slide with 1-2 drops of water (you do NOT need iodine for this sample). 2. Using a 45-degree angle, lower a cover slip onto the slide. 3. Begin on lower power, and then move up to higher power. Using detail and pencil, sketch what you see below. Don’t forget to record the total magnification used. III. Elodea (or other plant) STOMATES? Total Magnification: ____ X DISCUSSION QUESTIONS: (1 point per question) 1. Why does the specimen placed under the microscope have to be thin? 2. Why is it necessary to stain the prepared animal cells slides, but not the elodea? 3. Are the cells that you observed eukaryotic or prokaryotic? Explain why (remember what makes something eukaryotic and prokaryotic). 4. What are three characteristics that plant and animal cells have in common? 5. What are two differences between plant and animal cells? 1 Journal 4-4: Cells Alive – Internet Lesson Need version that used Genetics Utah website URL: www.cellsalive.com Objective: You will look at computer models of cells, learn the functions and descriptions of the cells and their components. Navigating the site: Cells alive has a navigation bar at the left. After accessing the page, click on CELL BIOLOGY on the left side navigation bar. From here, you will access the links: “How Big is a…,” the animal cell model, the plant cell model, and the bacterial cell model. Part A: “How Big is a…” Here you will look at objects on a virtual piece of paper. Your job is to find them and estimate the length of each (in picometers, nanometers, micrometers, or millimeters). (Note: 1 m = 1,000 mm = 1,000,000 micrometers = 1,000,000,000 nanometers) Object Size in picometers (pm), nanometers (nm), Eukaryotic or Prokaryotic micrometers millimeters (mm) Grain of rice Amoeba proteus Skin Cell Red Blood Cells Baker’s Yeast Mitochondrion N/A (an organelle) E. coli HIV N/A (viruses aren’t cells) Rhinovirus N/A (viruses aren’t cells) N/A (a protein) Hemoglobin 1. Which of the above is the largest? 2. Which of the above is the smallest? 3. Approximately how much smaller is a hemoglobin molecule compared to a red blood cell? 2 B. Cell Models: Comparing Prokaryotic (Bacteria) and Eukaryotic (Plant and Animal) Cells URL: www.cellsalive.com Navigating the site: Cells alive has a navigation bar at the left. After accessing the page, click on CELL BIOLOGY on the left side navigation bar. From here, you will access the links: the animal cell model, the plant cell model, and the bacterial cell model. Overview: For the chart below, place a check in the box if the cell has that cell part. Plant Animal Bacteria Chloroplast Vacuole Ribosome Mitochondria DNA Endoplasmic Reticulum Cell Wall Golgi Apparatus Now, click on Eukaryotic Cell Interactive Animation. Click on individual organelles. Use the boxes below to do a more in-depth study of the organelles. Organelle Where found (plant/animal/both) What it does in the cell Nucleus Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Nucleolus Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Cell Membrane Sketch Something that performs the same function 3 Organelle Where found (plant/animal/both) What it does in the cell Cytoplasm Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Ribosome Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Mitochondria Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Vacuole Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Golgi Body Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Rough Endoplasmic Reticulum (ER) Sketch Something that performs the same function 4 Organelle Where found (plant/animal/both) What it does in the cell Smooth Endoplasmic Reticulum (ER) Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Lysosome Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Cell Wall Sketch Something that performs the same function Organelle Where found (plant/animal/both) What it does in the cell Chloroplast Sketch Journal 4-5, Part 2: Prokaryotic and Eukaryotic Cells Do all cells have the same structure? An efficiency apartment is a one-room apartment. This one room is where you sleep, eat, shower, and entertain your guests. It all happens in one room. It is a simple way of living in a small space. A mansion is a large, complex living space with many separate rooms. There are rooms for cooking, eating, sleeping, bathing, reading, watching TV, entertaining guests, exercising, and storage. The rooms in a mansion are constructed for the specific things you would like to be able to do. You can live in simple efficiency or complexity. In this activity we will be looking at cells that are as simple as a one-room efficiency apartment or as complex as a mansion. 5 1. The three bacterial shapes in Model 1 are referred to as coccus (sphere), spirillum, and bacillus (rod). Label the diagrams in Model 1 with the correct descriptions. 2. What is represented by the small dots found in each of the bacteria cells? 3. What is the name of the outermost layer that forms a boundary around the outside of each cell? 4. How is the DNA described and what does this mean? 5. All the internal structures are suspended (floating) in what substance? 6. One of the bacteria in Model 1 has a tail-like structure. a. What is this structure called? b. What might be the purpose of this structure? c. Based on your answer to the previous question, what might you infer about the absence of this structure in the other two bacteria cells? 6 7. Looking at Model 2, list at least three structural differences (other than shape) between an animal and a plant cell. 8. Where do you find the DNA in each cell in Model 2? 9. Do both cells in Model 2 have a nucleus? 10. List the structure(s) that form the boundary between the inside and the outside of each cell in Model 2. 11. What is different about the outermost boundary in a plant cell compared to an animal cell? 12. Decide as a group whether the cells in Model 1 or 2 are more complex and list at least three supporting reasons for your choice. Model 3 – Structural Comparisons Word Part Meaning Pro karyon Eu Before Nucleus or kernel true 13. Use the chart in Model 3 to determine the meaning of the word prokaryote. 7 14. What does the word eukaryote mean? 15. Based on the above word definitions, label the cells in Model 1 and Model 2 as prokaryotic or eukaryotic. 16. By comparing Model 1 and Model 2, what structures are the same in both prokaryotic and eukaryotic cells? 17. What differences are there between a prokaryotic and eukaryotic cell? 18. Refer to Models 1 & 2 to complete the chart below. Write yes or no in the box for each cell. Bacterial Cell Animal Cell Plant Cell All Cells Cell Membrane Ribosomes Cytoplasm Mitochondria Nucleolus Nucleus DNA Cell Wall Prokaryote Eukaryote 19. As a group, write a definition for a prokaryotic cell. 20. As a group, write a definition for a eukaryotic cell. 21. Complete the phrase below. Each member must contribute one complete sentence. The words prokaryotic and eukaryotic must be used: All cells are not the same because... 22. As a group, discuss the opening analogy of an efficiency apartment and a mansion as it relates to cells. Record your final consensus of how this analogy applies to cell structure. 8 Extension Questions 23. What effect do you expect the structural differences between prokaryotes and eukaryotes to have on their functions? Explain in detail. 24. With as much detail as possible, give another example of an analogy for describing the difference between prokaryotic cells and eukaryotic cells. Journal 4-6: Doctor Diagnosis Diagnosis: Cells! Graduation is early this year! You have just been awarded your PhD in Cellular Biology and so your friends now call you “doctor.” One friend approaches you about problems she is having with her experiment (she has yet to graduate). She is using various cells and each of them seems to have a problem. She hands you a list of the problems and asks you to diagnose the issue. Make sure you state the problem organelle and what is wrong. Ex.. The cell has a ton of debris (junk) in the cytoplasm. --The lysosomes that digest useless material in the cell are missing or not functioning properly. 1. The sperm cells won’t move. 2. The plant cell is sluggish—it won’t produce enzymes and respond to stimuli. 3. The cell lacks proteins. 4. The organelles in the cell won’t move. 5. The organelles in the cell are not receiving 02. 6. The plant cells lack rigidity and the plants are drooping as a result. 7. The cell has proteins that are stuck in one area; they cannot leave the cell. 8. The plant cells cannot build up pressure for a plant to support itself. 9 9. The cell is not passing on its genetic information. 10. Muscle cells are not contracting. 11. The cell has no RNA or ribosomes. BSCS Biology Dragonfly Textbook Reading Guide Unit 4: Internal Environment - Cells Your notes should be written in your own words, showing that you understand the reading assignment and are not merely copying text from the book. Please be sure to look at the figures in the book in each section, especially using ones identified in the outline below. Chapter 7 Life is Cellular (Section 7-1, p. 169) A. State the 3 parts of the Cell Theory: B. Distinguish between Prokaryotes and Eukaryotes (p. 172) and give examples. Eukaryotic Cell Structure A. The Discovery of the Cell. 1. Who were Robert Hooke and Anton von Leeuwenhoek? What did they discover and when? 2. Who were the 2 scientists that developed the Cell Theory? 3. What are the 3 parts of the Cell Theory? 10 B. Read pages 174-181 to label the animal cell diagram below and describe the functions of the following organelles: cell wall, nucleus, nuclear envelope, cytoskeleton, cytoplasm, ribosomes, endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, chloroplasts, mitochondria, membrane, centriole, and cilium. Study Sheet for Unit 4: Internal Environment - Cells Use these questions below to help you review for the Test. Review Questions 1. Define homeostasis. 2. What are diffusion and osmosis? Give examples of these. What causes diffusion to occur? 3. Define isotonic, hypertonic, and hypotonic. Explain what happens to blood cells and onion cells when placed in isotonic, hypertonic, and hypotonic solutions. Explain why these changes occur. 4. Explain how molecular size influences whether or not a substance can pass through a membrane. 5. Distinguish between the following terms: permeable, impermeable, & selectively permeable. 6. What is the difference among passive transport, facilitated diffusion, and active transport? 7. What are the types of active transport? Know: membrane-associated pumps, endocytosis, exocytosis, phagocytosis, and pinocytosis. 8. What is the chemical make-up of the cell membrane? Explain what is meant by “lipid bilayer.” 9. Why is the membrane called a fluid-mosaic model? 10. Define hydrophobic and hydrophilic and apply these terms to the components of the cell membrane. What type of molecules can and cannot pass through the membrane? 11. Explain why cells MUST be small. Use the concept of surface area to volume ratio in your answer. 12. What do plant cells have in common? What do animal cells have in common? Compare and contrast plant and animal cells. 13. What are the internal compartments (organelles) in a cell? What are the functions of each of the organelles of the cell? Be able to identify these on a diagram. 11