OHS026
Safe Work Procedure
Faculty/Division
Medicine
Document number
SOMS GBD SWP 020
School/ Divisional Unit
School of Medical Sciences
Initial Issue date
13-03-2009
Current version
1.0
Current Version
Issue date
20-04-2010
Next review date
20-04-2013
The Writing Safe Work Procedures Guideline (OHS027) should be consulted to assist in the completion of this
form.
Safe Work Procedure Title and basic description
Protein extraction
Title:
Description: extract protein from human or animal tissue
Associated risk assessment title and location: RA Handling of human tissue 020, RA Bench top centrifuge 003, RA Waste
management 015 located in room 209B
Describe the activity or process
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Weigh samples (100mg) and place it in 1ml protein extraction buffer in a special, GeneWorks homogenizer centrifuge
tube.
Homogenize the samples.
Centrifuge the samples at 12000g for 15min at 4oC.
Transfer the supernatant to a fresh tube.
Measure protein quantitatively by spectrophotometer with Lowrys method.
Add 200ml of the suspension or sample to the cuvette.
Make up protein standards.
Add 1ml of lowrys base solution to each cuvette, leave for 10min.
Add 0.1ml of color solution to each cuvette, mix thoroughly, leave for 20min to develop the color.
Read absorbance on the spectrophotometer at wavelength 750nm.
Store protein samples at -80.
List all resources required including plant,
chemicals, personal protective clothing and
equipment, etc
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Suitable PPE
Protein extraction buffer, lowrys solution and standard BSA
Centrifuge tubes, samples, scissors, forceps, 70% ethanol spray bottle
Spectrophometer, homogenizer and centrifuge
List potential hazards and risk controls including
specific precautions required
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Chemicals may be hazardous – for chemicals check MSDS and wear PPE, all users are well trained and all spills are
cleaned immediately and disposed in chemical waste.
Tissue can be contaminated or infectious – training is required if handling human tissue, wear PPE, all spills are cleaned
immediately with 70% ethanol and disposed in autoclaved waste. Scissors and spatula are wiped with 70% ethanol and
washed with water. All weight boats and tubes are disposed in biological waste.
Potential electrical hazard – do annual inspections of electrical equipment and ensure electrical safety tag is current.
Potential aerosol hazard – wear PPE and use a biosafety hood or wear a mask if it is necessary.
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Page 1 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007
List emergency shutdown instructions
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Spectrophometer, homogenizer and centrifuge have “OFF” switch that can be used in emergency.
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Shut all electrical equipment off at power point in case of emergency.
List clean up and waste disposal requirements
For chemicals:
Clean up with detergent and then with water
Dispose into chemical waste
For samples:
Clean up or sock with 70% ethanol
Dispose into biological waste or in autoclaved waste (human sample)
List legislation, standards and codes of practice used
in the development of the SWP
NSW OHS Act 2000
NSW OHS Regulation 2001
Australia Dangerous Goods Code
Code of Practice for the Labeling of Workplace Substances
AS/NZS 2243.2:2006. Safety in laboratories. Part 2: Chemical aspects
Australian Standard AS2243.6-1990. Safety in laboratories. Part 6: Mechanical Aspects.
Australian Standard AS2243.7-1991. Safety in laboratories. Part 7: Electrical Aspects.
AS/NZS 2161.1:2000 Occupational Protective Gloves – Selection, Use and Maintenance
Safe Work Procedure Form (OHS026)
UNSW Hazardous Waste Disposal Procedure
Supervisory approval, training, and review
Supervisor: Prof E Burcher
Signature:
Plant custodian:
Signature
List competency required – qualifications, certificates, licensing, training - e.g. course or instruction:
SWP review date: 20-04-2013
Responsibility for SWP review: Fei Shang
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Page 2 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007