AIS Chapter 6 Blood lactate thresholds 1 Production of Lactic Acid (lactate) • Normally, O2 is available in the mitochondria to accept H+ (and electrons) from NADH produced in glycolysis – In anaerobic pathways, O2 is not available • H+ and electrons from NADH are accepted by pyruvic acid (pyruvate) to form lactic acid 2 Conversion of Pyruvic Acid to Lactic Acid • Recycling of NAD (NADH NAD) • So that glycolysis can continue • LDH: lactate dehydrogenase 乳酸去氫脢 Blood lactate testing rationale • Indicators of training adaptation – Adapts to a greater degree than VO2max – Especially true in highly trained athletes • Correlate with endurance performance – Better indicator than VO2max • Optimal training stimuli 4 Lactate response after training adaptation 5 Lactate response after training adaptation 6 Definitions of lactate threshold • Various terminology – Anaerobic threshold, aerobic-anaerobic threshold, onset of blood lactate accumulation (OBLA) • Lactate threshold 1 (LT1) – First exercise intensity at which there is a sustained increase in lactate above resting level – Usually < 2.0 mM • Lactate threshold 1 (LT2) – First exercise intensity at which there is a very rapid increase in lactate – Shift from oxidative to partly anaerobic energy – 2.5-5.5 mM 7 LT1 and LT2 8 Fixed lactate concentration 2.0, 2.2, 2.5, 3.0, 4.0 mM 9 Individualized lactate threshold Lactate E for ADAPT: http://www.nuigalway.ie/maths/jn/LACTATE/html/lactate-e.html 10 Test protocols • Incremental duration: 5-8 min – < 5 min may overestimate LT2 – Especially the purpose is to prescribe exercise intensity for endurance training • Rest interval duration: 0-60 s – For blood collection and/or modify equipment • Number of increments: 5-7 – Relatively small increments in intensity: 1-2 km/hr per stage (~1 km/hr perferred) 11 Lactate concentrations in difference durations of stages 12 LT2 occurs at different lactate concentrations for difference exercise modes 13 Lactate concentration in muscle and blood • Strong correlation between muscle and blood lactate concentration • Blood lactate NOT wholly reflective of muscle lactate • High muscle lactate requires significant time lag before lactate equilibrates with blood – Require longer (> 5 min) stage duration 14 Practical applications • Use individually measured LT1 and LT2 • Prescribe endurance training intensities – – – – – T1: light aerobic T2: moderate aerobic T3: heavy aerobic T4: anaerobic threshold T5: maximal aerobic • Evaluate training adaptations – Graphical overlays for subjective assessment – ↑intensity at LT1: ↑ base aerobic condition – ↑intensity at LT2: ↑high-level aerobic endurance 15 Endurance training zones 16 Training adaptations in lactate concentration 17 Homework • Design protocols for running MAOD (p67-68) • Design protocols for running lactate threshold (p84-89) • Design protocols for running VO2max (p113114) • Chapter 26 Runners and walkers (p401-408) 18