Cover Page
The handle http://hdl.handle.net/1887/28970 holds various files of this Leiden University
dissertation.
Author: Raterink, Robert-Jan
Title: High-throughput profiling of small molecules using mass spectrometry
Issue Date: 2014-10-01
High-Throughput Profiling of Small Molecules
using Mass Spectrometry
Proefschrift
ter verkrijging van
de graad van Doctor aan d­­e Universiteit Leiden
op gezag van Rector Magnificus prof. mr. C.J.J.M. Stolker,
volgens besluit van College voor Promoties
te verdedigen op woensdag 1 oktober 2014
klokke 13:45 uur
door
Robert-Jan Raterink
geboren te Dronten
in 1982
Promotiecommissie:
Promotor:
Prof. dr. Thomas Hankemeier
Co-promotor:
Dr. Rob Vreeken
Overige leden:
Prof. dr. Albert van den Berg (Twente University)
Dr. Christa Cobbaert (LUMC, Leiden)
Prof. dr. Meindert Danhof (Leiden University)
Prof. dr. Robert Kennedy (University of Michigan)
Prof. dr. Dirk Jan Reijngoud (University of Groningen)
Prof. dr. Herman Spaink (Leiden University)
This study was financed by the research programme of the Netherlands Metabolomics
Centre (NMC) which is a part of The Netherlands Genomics Initiative/Netherlands
Organization for Scientific Research.
Publication of this thesis was financially supported by ZF-Screens B.V.
Cover illustration: Gilberto Gennero
Printed by Ipskamp Drukkers B.V.
High-throughput profiling of small molecules using mass spectrometry
RJ Raterink
PhD thesis, Leiden University
ISBN: 978-90-74538-86-2
Table Of Contents
Chapter 1
Chapter 2
General Introduction
5
Recent Developments in Sample Pretreatment
13
Techniques for Mass Spectrometry-based Metabolomics
Chapter 3
Rapid Metabolic Screening of Early Zebrafish Embryogenesis based on Direct-Infusion-nanoESI-FTMS
33
Chapter 4
Three-Phase Electroextraction: a New (Online) Sample Purification and Enrichment Method for Bioanalysis
47
Chapter 5
Gas Pressure Assisted Micro-Liquid-Liquid Extraction 63
coupled Online to Direct Infusion Mass Spectrometry: a New Automated Screening Platform for Bioanalysis.
Chapter 6
Testing Tuberculosis Drug Efficacy in a Zebrafish High-
Throughput Translational Medicine Screen
77
Chapter 7
Conclusions and Perspectives
Samenvatting
97
101
Appendix
Dankwoord
Curriculum Vitae
List of publications
105
106
107
108