Cover Page
The handle http://hdl.handle.net/1887/28538 holds various files of this Leiden University
dissertation
Author: Schimmel, Joost
Title: Regulation of genome stability and cell cycle progression by SUMOylation
Issue Date: 2014-09-09
Regulation of genome stability and
cell cycle progression by
SUMOylation
Joost Schimmel
Copyright © 2014 by J. Schimmel. All rights reserved.
Copyright of the individual chapters rests with the authors with te following
exceptions:
Chapter 1: John Wiley & Sons, Inc
Chapter 2: the American Society for Biochemistry and Molecular Biology
Chapter 4 and 5: Elsevier - Cell Press (Molecular Cell)
Regulation of genome stability and
cell cycle progression by
SUMOylation
proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus Prof. mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties
te verdedigen op dinsdag 9 september 2014
klokke 13.45 uur
door
Joost Schimmel
geboren te Lopik
op 17 april 1984
Promotiecommissie:
Promotor:
Prof. dr. P. ten Dijke
Copromotor:
Dr. A.C.O Vertegaal
Overige leden:
Prof. dr. T.K.Sixma (NKI, Amsterdam)
Prof. dr. G.J.P.L. Kops (UMC, Utrecht)
Dr. P. Knipscheer (Hubrecht Instituut, Utrecht)
The studies described in this thesis were performed at the department of Molecular
and Cellular Biology, Leiden University Medical Center and was financed by the
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO).
Cover: Painting by Hiroyuki Kimura. Photographed by Naosuke ii
With kind permission of both artist and photographer.
Printed by: Proefschriftmaken.nl || Uitgeverij BOXPress
Published by:
Uitgeverij BOXPress, 's-Hertogenbosch
ISBN:978-90-8891-919-0
The printing of this thesis was financially supported by the J.E. Jurriaanse Stichting
and Greiner Bio-One.
"I was just guessing at numbers and figures,
pulling the puzzles apart.
Questions of science, science and progress
do not speak as loud as my heart."
- Coldplay, The Scientist -
aan mijn familie
voor Karin
Table of contents
Aim and outline of the thesis
9
Chapter 1
Introduction
13
Chapter 2
The ubiquitin-proteasome system is a key component of the SUMO-2/3 cycle 41
Chapter 3
USP11 Counteracts RNF4 and Stabilizes PML Nuclear Bodies
63
Chapter 4
Site-Specific Identification of SUMO-2 Targets in Cells Reveals an Inverted SUMOylation Motif
and a Hydrophobic Cluster SUMOylation Motif
87
Chapter 5
Uncovering SUMOylation Dynamics During Cell Cycle Progression Reveals FoxM1 as a Key Mitotic
SUMO Target Protein
117
Chapter 6
The Cockayne Syndrome-B protein is SUMOylated
upon UV induced DNA damage 155
Chapter 7:
Summary and discussion181
Chapter 8:
Nederlandse samenvatting
195
List of abbreviations
201
Curriculum Vitae
207
List of publications
209