List of posters Bioinformatics P01: MsCompare: A Software Suite for LC and GC/MS Metabolomics M.M.A. Ruijken P02: Bioinformatic tools for the analysis of protein complexes enriched by sizeexclusion chromatography in label-free and SILAC labeled cells Linsey M. Raaijmakers, Renske Penning, Nadine Binai, Maarten Altelaar, Albert J.R. Heck P03: Automated Differential Pattern Discovery Across Multiple Tissue Samples Measured by Imaging Mass Spectrometry Nico Verbeeck, Yousef El Aalamat, Bart De Moor, Richard M. Caprioli, Raf Van de Plas, and Etienne Waelkens Current applications for large molecules P04: A TransOmics study of the saliva of children with autism spectrum disorder using high definition mass spectrometry Alisa G. Woods; Joanne B. Connolly ; Lee Gethings; Armand G. Ngounou Wetie; Cristian Cojocariu; Janet Hammond; Jeanne P. Ryan; Costel C. Darie P05 : On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases Reggy Ekkebus, Sander I. van Kasteren,Yogesh Kulathu, Arjen Scholten, Ilana Berlin, Paul P. Geurink, Annemieke de Jong, Soenita Goerdayal, Jacques Neefjes, Albert J. R. Heck, David Komander, and Huib Ovaa P06: LC-MS analysis of intact enzymes using the Synapt G2 mass spectrometer Ioana Barbu, Nicolas Abello, Jort Gerritsma, Maurien Olsthoorn, Marcel van Tilborg P07: Controlled Reduction of Disulfide Bonds in Biopharmaceuticals Using an Electrochemical Reactor Cell online with LC/MS Agnieszka Kraj, Hendrik-Jan Brouwer, Nico Reinhoud, Jean-Pierre Chervet P08: Comprehensive Study of O-Linked Glycans of Erythropoeitin by nano-LC-MALDI TOF/TOF MS Patrick van Houtsa, Anja Resemannb, Nannan Taoc, Ulrike Schweiger-Hufnagelb, Kristina Marxb and Stephanie Kaspar P09 : Glycan and glycopeptide profiling of biopharmaceuticals D. Falck, K. Reiding, A. L. Hipgrave Ederveen, G.S.M. Kammeijer, M. Wuhrer P10: Evaluation of a new combination integrating an ion funnel and an active pixel detector for the efficient transmission and detection of native proteins generated by electrospray ionization Tiffany Porta, Shane R. Ellis, Dirk-Jan Spaanderman, Marc C. Duursma, Ron M.A. Heeren P11: A High Resolution Bench-Top Orbitrap LC-MS Workflow Solution for Comprehensive Intact Monoclonal Antibody Characterization Zhiqi Hao, David Horn, Shiaw-Lin Wu, Xiaoyue Jiang, Andreas FR Huhmer and Patrick K Bennett P12: Large Scale Targeted Protein Quantification using HR/AM Selected Ion Monitoring with MS/MS Confirmation on the Orbitrap Fusion Tribrid MS R. Kiyonami, M. Senko, V. Zabrouskov, J. Egertson, S. Ting , M. MacCoss, A. FR Hühmer P13: Localizing and characterizing disulfide bonds in MS-based proteomics using online LC–Electrochemistry–MS L. Switzar, S. Nicolardi, H. Dalebout, A. Aartsma-Rus, M. Palmblad, Y. van der Burgt P14: The behaviour of detergent micelles in the gas phase, analyzed by Ion mobility mass spectrometry Jeroen van Dyck, Albert Konijnenberg, Frank Sobott P15: Validation study of a targeted LC-MS method for the detection of milk and egg allergens in cake flour Merel A. Nessen, Klaas Wubs, Dick Hooijerink, Stephen Lock, Monique G.E.G. Bremer, Saskia Sterk Current applications for small molecules P16: Improved detection of thiouridine analogs Jef Rozenski P17: Quan / Qual Methodology for P450 Drug and Metabolite Profiling Don Richards, Helen Lomax, Mark Savage, Angus Nedderman P18: Analysis of black tea by high-resolution UPLC-MS Boudewijn Hollebrands, Christian H. Grün P19: Hydrogen peroxide reactions on cocaine in hair using imaging mass spectrometry Eva Cuypers, Bryn Flinders, Ingrid Bosman, Klaas Lusthof, Arian Van Asten, Jan Tytgat, Ron M.A. Heeren P20: Combined mass spectrometry and multivariate data analysis image accumulation of fatty acids in the hindbrain of mouse models with impaired peroxisomal β-oxidation Karolina Škrášková, Gert Eijkel, Myriam Baes, Paul P. Van Veldhoven, Stephanie de Munter, Ron M.A. Heeren P21: High Performance Anion Exchange Chromatography (HPAEC)-Mass Spectrometry (MS): analysis of complex mixtures of oligosaccharides in biomass hydrolysates Leon Coulier, Wibo van Scheppingen, Rob van der Hoeven, Jort Gerritsma P22: Breath acetone to monitor life style interventions in field conditions: An exploratory study D.Samudrala,J.Mandon,P.A.Brown,G.Lammers,M.T.E. Hopman, L.Blanchet, F. J. M. Harren,S.M.Cristescu P23: NPLC-APPI-MS for the separation and identification of sterols, steradienes, and sterol oxides Yvonne Westphal, Sophie Besseau, Christian Grün P24: Detecting meat and fish spoilage compounds using Proton Transfer Reaction Mass Spectrometry P. Brown, D. Samudrala, F. J. M. Harren, S. M. Cristescu P25: Nutrition Researcher Cohort and metabolomics analysis in dry blood spot samples I.Bobeldijk, E. Verheij, A. Boorsma, Th. Gundersen, L. Brennan, B. van Ommen, H. Daniel, L. O. Dragsted, J. Bouwman, M. Caspers, S. Wopereis, L. Schomburg, E. Bakaeva and K. E. Geillinger P26: Ultrasensitive Quantification Assay for Oxytocin in Human Plasma using the Waters ionKey™ LC/MS Microfluidic Platform Eric S.E. van Beelen, Catalin E. Doneanu, Paul Rainville P27:Quadrupole-Orbitrap Steroid Analysis in Bovine Urine Frans Schoutsen, Marco Blokland, Paul Zomer P28: Preparation of longitudinal sections of hair samples for analysis by multi-modal mass spectrometry imaging Bryn Flinders, Eva Cuypers, Hans Zeijlemaker, Ron M. A. Heeren P29: Evaluation of different column chemistries for fast urinary metabolic profiling D. Kloos, H. Lingeman, O.A. Mayboroda, W.M.A. Niessen, A.M. Deelder, M. Giera P30: Accurate mass isotopomer determination of TMS-MOX derivatized central carbon metabolites in industrial microorganisms using GC-MS/MS A. ten Pierick, P.T.N. van Dam, S.A. Wahl, R. Maleki Seifar P31:The first step towards a Quan/Qual workflow: chromatographic peak capacity optimization Anne-Charlotte Dubbelman, Filip Cuyckens, Lieve Dillen, Gerhard Gross, Thomas Hankemeier, Rob J. Vreeken P32: Probing the innate immune response through viral metabolomics: An in vitro feasibility study J.C. Schoeman, K. Strassburg, F. Zaaraoui, A. Andeweg, A.B. Osterhaus, T. Hankemeier, R.J. Vreeken Identification P33: Pre-fractionation of protein complexes using Size Exclusion Chromatography R.Penning, LM Raaijmakers, NA Binai, AJR Heck, AFM Altelaar P34: A Proteomics approach to study the changes in the interactome of Protein Phosphatase 1 in Arterial Fibrillation David Y. Chiang, Nicolas Lebesgue, David L. Beavers, J. Mirjam A. Damen, Niels Voigt, Dobromir Dobrev, Albert J.R. Heck, Xander H.T. Wehrens, Arjen Scholten P35: Ultrafast Statistical Profiling: FTMS based profiling of Myxobacteria natural products for rapid detection and identification of marker compounds Rob van der Heijdena, Aiko Barschb; Matthias Wittb, Daniel Krugc,d,Thomas Hoffmannc,d and Rolf Müllerc,d P36: Robust phosphoproteome enrichment using titanium (IV) affinity chromatography Houijang Zhou, Harm Post, Hanfa Zou, Shabaz Mohammed, and Albert J.R. Heck P37: Molecular characterisation of food emulsifiers using combined liquid chromatography – mass spectrometry Wilma du Chatinier and Hans-Gerd Janssen P38: Metabolomics of Opiate-induced changes in Murine Brain by GC/Q-TOF. Martin Haex; Sofia Aronova, Manhong Wu, Peyman Sahbaie, Ming Zheng, David Clark, Stephan Baumann and Gary Peltz P39: Characterisation of exosomes in colon cancer cells Lim 1215 highlights the existence of exosomes subpopulations. Antoine Hubert, Bart Jacobs, Rita Derua, Jens Wouters, Ben Van den Bosch, Sabine Tejpar and Etienne Waelkens. P40: Enhancing General Unknown Screening with Data Independent Analysis on a Quadrupole Orbitrap Mass Spectrometry System Olaf Scheibner, Matthias Ruff, Bernadette Vogler, Heinz Singer, Maciej Bromirski P41: Stabilization of sialylated N-glycans by methyl esterification and lactonization for analysis by MALDI-TOF-MS D.M. Kuijper, K.R. Reiding and M. Wuhrer Non-mainstream P42: Lipidomic Profiling of Rat Adipose Tissue after Treatment with PPAR-pan Agonist Using Sub-2µm Particle CO2 Based Supercritical Chromatography Mass Spectrometry Giorgis Isaac, Michael D. Jones, James Langridge, John P. Shockcor, and Julian L. Griffin P43: Spectroscopic identification of cyclic diketopiperazine b2-ions from deprotonated peptides containing proline and histidine Josipa Grzetic, Jonathan Martens, Giel Berden, Jos Oomens P44: Mass Spectrometry Imaging of Breast Tumor Hypoxia Using the Chemical Marker Pimonidazole Nadine E. Mascini, Asif Rizwan, Lu Jiang, Menglin Cheng, Kristine Glunde, Ron M.A. Heeren P45: Development of an isotopic traceability tool for the geographical origin of bell peppers Eva de Rijke, Chiara. Cerli, Daniël Bakker, Michel Ebskamp, Karsten Kalbitz, Chris de Koster P46: Native mass spectrometry: what can it bring to you? Anja Boumeester, Arjan Barendregt, Sara Rosati, Albert J.R. Heck P47: Molecular Diversity and Body Distribution of Saponins in the Sea Star Asterias rubens by Mass Spectrometry Demeyer Marie, Caulier Guillaume, De Winter Julien, Wisztorski Maxence, Fournier Isabelle, Eeckhaut Igor, Flammang Patrick and Gerbaux Pascal P48: Electrochemistry/MS – a Powerful Tool in Drug Metabolism Agnieszka Kraj, Hendrik-Jan Brouwer, Nico Reinhoud, Jean-Pierre Chervet P50: MALDI-TOF mass spectrometry imaging of drug related crystal-like structures in frozen and paraffin embedded formalin-fixed rabbit kidney tissue sections Anne L. Bruinen, Gert Eijkel, Marjolein Van Heerden, Filip Cuyckens, Ron M.A. Heeren P51: Qualitative and Semi-Quantitative Analysis of Intact Chicken Ovalbumin by HighResolution Native Mass Spectrometry Reveals 59 Proteoforms Arjan Barendregt, Yang Yang and Albert J.R. Heck. P52: A Multi-Analytical Approach for the Architectural Purity Assessment of Macrocyclic Polyesters Obtained by Diels-Alder Photo-Click Coupling Thomas Josse, Ozcan Altintas, Julien De Winter, Kim K. Oehlenschlaeger, Philippe Dubois, Olivier Coulembier, Christopher Barner-Kowollik and Pascal Gerbaux P53: Comprehensive characterization of the mouse brain proteome sampled in mass spectrometry imaging studies. Bram Heijs, Ricardo Carreira, Reinald Shyti, Arn van den Maagdenberg, Peter van Veelen, Liam McDonnell P54:Control over Molar mass, Dispersity, End-groups and Kinetics in Cyclopolymerization of ortho-Phthalaldehyde : Adapted Choice of Phosphazene Organocatalyst. Julien De Winter, Andrew P. Dove, Armin Knoll, Pascal Gerbaux, Philippe Dubois and Olivier Coulembier P55: Chemical reactions of cross-linked peptides in solution and in the gas phase for topology studies of macromolecular assemblies in complex mixtures Luitzen de Jong, Hansuk Buncherd, Winfried Roseboom, Leo J. de Koning and Chris G. de Koster P56: One-pot microscale sample preparation for Capillary Electrophoresis and Mass Spectrometry Anthonius A. M. Heemskerk, Guinevere S.M. Kammeijer, Bart Schoenmaker, Rico J.E. Derks, André M. Deelder, Oleg A. Mayboroda P57: Surface analysis for compositional, chemical and structural imaging; hydrocarbon search in sedimental rocks Guzel Kireeva, Marc C. Duursma, Bryn Flinders, Peter Arisz, Herman P.C.E. Kuipers, Pim Mul, Erik Tegelaar, Ron M.A. Heeren P58: Quadrupole Mass Filter design and performance for operation in stability zone 1 1Mariya J. Antony Joseph, 2Sarfaraz U. Syed, 1Simon Maher, 1Fred P. M. Jjunju, 2Ron Heeren, and 1Stephen Taylor* P59: A new approach in hair forensics: Rapid analysis of THC in hair using DARTāMS W.F. Duvivier, T.A. van Beek, E.J.M. Pennings, M.W.F. Nielen P60: Mass spectrometry imaging of crude oil interfaces with water/brine and surfactants Mark Jansena, Marc Duursmaa, Donald F. Smitha,b, Pim Mulc, Herman Kuijpersc, Erik Tegelaard, Frans Korndorfferd, Jos Pureveend, Ron M.A. Heerena P61: Fundamental spray characteristics and complex formation using SAWN and ESI Bob Hommersom, Shane R. Ellis, Tiffany Porta, Ron M.A. Heeren P62: History of mass spectrometry as mapped by bibliometric methods Cathelijn Waaijer and Magnus Palmblad P63: Molecular mapping of skin and biofilms by MALDI MS imaging Boudewijn Hollebrandsa, Hans-Gerd Janssena,b, Christian H. Grüna Quantitation P64: Disclosure of efflux pumps in the proteome of the Mycobacterium tuberculosis Beijing genotype Jeroen de Keijzer, Petra de Haas, Arnoud H. de Ru, Peter A. van Veelen, Dick van Soolingen P65: Maturation reinforcement of Bacillus subtilis spores by cross-linking of spore outer coat proteins Wishwas Abhyankar , Rachna Pandey, Alexander Ter Beek b, Stanley Brul , Leo J. de Koning , Chris G. de Koster P66: Identification of post-transcriptional control upon Carbon Catabolite Repression in Escherichia coli O. Borirak, M.D. Rolfe, M. Bekker, H. Dekker, W. Roseboom, J. Green, K.J. Hellingwerf, H.C.J. Hoefsloot, L.J. de Koning, and C.G. de Koster P67: Applying Micro Flow LC and High Speed Data Acquisition MS/MS to the Analysis of Pesticides Residues in Complex Spice Matrix V. Goudriaana, D. Bakerb, N. Loftusb, S. Hirdc P68: Rapid Simultaneous Assay of 25 Mycotoxins in a Variety of Food Samples V. Goudriaana, S. Moreaub, M. Levi, E. Capodannoc P69: Direct Monitoring of Cerebellar Phosphorylation Dynamics in a Kinase Knockout Mouse Eleonora Corradini, Raghavan Vallur, Linsey M. Raaijmakers, Susanne Feil, Robert Feil, Albert J.R. Heck, Arjen Scholten P70: Throwing DARTs at the surface Rickdeb Sen, Han Zuilhof P71: Maximizing Triple Quadrupole Mass Spectrometry Productivity Through the Automated Use of an Expanded Dual-Channel HPLC System with Online Sample Cleanup Martin Haex, Kevin McCann, Sameer Nene, Doug McIntyre, Edmond Neo, Dennis Nagtalon; Agilent Technologies, Santa Clara, CA P72: A New Workflow for the Visualization and Identification of Lipids by LC Ion Mobility Mass Spectrometry (IMMS) Using IMS-QTOF with Fragmentation Martin Haex; Crystal K. Cody; Theodore Sana; Ruwan Kurulugama; William Barry; Alex Mordehai; George Stafford; Agilent Technologies P73: Quantitation and characterisation of cardiolipin molecular species by enhanced resolution (ER) mass spectrometry combined with MRM-induced tandem mass spectrometry on a hybrid triple quadrupole/ion trap (4000 QTRAP) mass spectrometer Derua, R,*., Daniels, V,*., Dehairs, J., Swinnen, J. and Waelkens, E P74: LC-MS/MS analysis of aromatic amines Lieve Dillen, Willy Cools, Michel Carpentier, Bart Stoops and Gerhard Gross P75: Advances in Sensitivity, Robustness, Speed and Dynamic Range on a Newly Developed Triple-Stage Quadrupole Mass Spectrometer Harald Osera, Ethan Chana, Oleg Silivraa, Joshua Mazea, Satendra Prasada, Jean-Jacques Dunyacha, Mike Belforda, Eloy Woutersa, Huy Nguyena, Terry Olneya P76: A comprehensive solution for quantitation of ketamine and ketamine metabolites in urine using LC-MS/MS and GC-MS/MS Helen Sun, Louis Maljers P77: N-Lactoyl amino acids - A novel class of mammalian metabolites formed by reverse proteolysis of lactate and amino acids R.S. Jansen, R.D. Addie, R. Merkx, A. Fish, O. Bleijerveld, P. Borst and K. van de Wetering