OPTICAL SCAN RELAY IN
CONFOCAL LASER SCANNING
MICROSCOPY
Student “#2”
Simplified Optics of a Confocal Microscope
MERITS OF CONFOCAL MICROSCOPY
a) the ability to serially produce thin (0.5 to 1.5 micrometer) optical
sections through fluorescent specimens that have a thickness
ranging up to 50 micrometers or more.
b) Image information is restricted to a well-defined plane, rather than
being complicated by signals arising from remote locations in the
specimen.
c) Contrast and definition are dramatically improved over widefield
techniques due to the reduction in background fluorescence and
improved signal-to-noise.
d) eliminates artifacts that occur during physical sectioning and
fluorescent staining of tissue specimens for traditional forms of
microscopy.
MERITS OF CONFOCAL MICROSCOPY
MERITS OF CONFOCAL MICROSCOPY
APPLICATIONS:
a) widely-used in biological science disciplines, from cell biology and
genetics to microbiology and developmental biology.
b) evaluation of various eye diseases, and is particularly useful for
imaging, qualitative analysis, and quantification of endothelial
cells of the cornea
c) endoscopic procedures: In the pharmaceutical industry, it was
recommended to follow the manufacturing process of thin film
pharmaceutical forms, to control the quality and uniformity of the
drug distribution.
d) data retrieval mechanism in some 3D optical data storage
systems and has helped determine the age of the Magdalen
papyrus.
DESIGN POINTS OF SCAN RELAY
In my design:
Lens ABCD: 32319 Edmund optics
(deameter =25mm)
Lens E: 36187 Edmund optics
(deameter=8mm)
Fa=Fb=Fc=Fd=27.5mm
Fa’=Fb’=Fc’=Fc’=34.2mm
D=1mm
Fe=3.3mm
Fa
A
Fa’
Telecentric lens
Fb’
B
C
Fb
Fc
D
Fc’
Fd’
E Fe
Fd
D