North Seattle Community College
Spring 2012
NANO230
Due:________________________
Homework 2
Read 11.1-11.4.1 pp 259-314. In Materials Characterization Techniques. Sam Zhang,
Lin Li, and Ashok Kumar.
1. Using a cartoon or diagram explain in a short paragraph how fluorescence
works. Show the difference between excitation wavelength and emission
wavelength. Describe two or more materials that display optical fluorescence.
2. Optical microscopy can be broadly divided into three categories: transmission
microscopy, reflection microscopy, and fluorescence microscopy. For each of the
three types, draw a rough cartoon indicating how each works. Label the parts of
your cartoon.
3. Draw a simple diagram which shows the working principals of confocal
microscopy. Label the parts of your diagram, and write a short paragraph or
numbered list explaining how confocal microscopy works.
4. Refer to table 11.2 on p 310. What is the maximum resolution of a confocal
microscope equipped with a PlanApo oil immersion 60x magnification lens and a
405 nm diode laser?
5. Laser Scanning Confocal Microscopes (LSCM), Atomic Force Microscopes
(AFM) and Scanning Electron Microscopes (SEM) are raster imaging methods.
Light microscopes and Transmission Electron Microscopes (TEM) are ensemble
imaging methods. Explain the difference between the two types of imaging. Draw
two diagrams to support your description.