2007 Syllabus for Anatomical Methods (ANAT 740)
Lectures – Wednesday 10-11:30; Room B60 (IRF Conference Room)
Labs – TBA for each unit
Introduction: Week 1 (January 16-20)
Lecture January 17 – Price
1) Course requirements
2) Rules for use of Instrumentation Resource Facility equipment and supplies
Labs – Set up times for training on microtomes and processing of tissues for LM.
Light Microscopy: Weeks 2-5 (January 21 – February 17)
Lecture January 24 – Borg: Overview of imaging techniques and light microscopy techniques
Lecture January 31 – Potts: Whole mount in situ and stereomicroscopy
Lecture February 7 – Price: Digital Imaging
Lecture February 14 – Price: Digital Imaging
Light Microscopy Labs – TBA: Benny Davidson
1) Tissue fixation
2) Paraffin and Cryostat Sectioning
3) Hematoxylin and Eosin Staining
4) PAS and/or trichrome staining
5) Prepare written report complete with stained slides and printed images
Digital Imaging Labs – TBA: Price and Jeff Davis
1) Use of light microscopes and digital cameras
2) Use of Adobe Photoshop and Powerpoint
3) Intro to Image Pro Plus
Prepare prints and slides using Photoshop and Powerpoint. You should submit prints of your work in the
written report and also submit a disk with a Powerpoint presentation showing prepared slides.
Reports for Light Microscopy and Digital Imaging are due on March 14. Turn reports in to Dr. Price.
Confocal Microscopy: Weeks 6-8 (February 18-March 10)
Lecture February 21 – Price: Confocal Microscopy
Lecture February 28 – Price: Confocal Microscopy
Lecture March 7 - Price: Confocal Microscopy
Confocal Labs – TBA: Price, Fuseler and Davis
1) Overview of confocal microscope operating software
2) Staining techniques for cell cultures and tissue
3) Imaging of double and triple labeled samples
4) Complete required exercises involving image acquisition and filter mechanisms
5) Use Voxblast or AMIRA to create a simple movie of confocal data set
6) Prepare report complete with prints.
Reports for Confocal Microscopy are due on March 21. Turn reports in to Dr. Price.
Week 9: Spring Break – March 11-17
Analysis of RNA, DNA and Proteins: Weeks 10-12 (March 18-April 7)
Lectures March 21, 28 and April 4 – Potts: Agilent 2100 Bioanalyzer, Real Time PCR, Bioplex Analysis;
Kodak ISM 2000; Phosphorimaging
Labs – TBA: Potts
1) Overview of Technology
2) Sample preparation
3) Complete assigned tasks on various pieces of equipment
Reports due to Dr. Potts on April 18
Flow Cytometry: Week 13 (April 8- April 14)
Lecture April 11 – Mayer: Epics XL Flow cytometer
Flow Cytometry Labs – TBA: Mayer
1) Complete designated exercises with the flow cytometer
2) Prepare report complete with graphs and analysis of data.
Reports for Flow Cytometry are due on May 2. Turn reports in to Dr. Mayer.
Laser Capture Microanalysis: Week 14 (April 15-April 21)
Lecture and Demonstration April 18 – Buckhaults (SCCC)
No Reports Due
DNA Microarrays: Week 15 (April 22-28)
Lecture and Demonstration April 25 – Creek (SCCC)
No Reports Due
Written Reports
Introduction:
- A brief description of the technology used in the section.
- Why the technology is important in the grand scheme of things – what types of data can be
acquired and why this is important in biomedical research
Materials and Methods
- Types of equipment used (Equipment names and model numbers are appropriate)
- Supplies used
- Protocols for specimen preparation
Results
-
A brief written description of the results you obtained
Prepared plates of images/data complete with figure numbers, labels, scale bars when
appropriate and legends
Discussion
- How bad did you mess up – give a critical appraisal of your results and if they did not turn out
as well as you expected tell how you might improve them
- How might this technology apply to your anticipated program of study?
- Anything you may want to add (within reason)
Requirements for an “A” in the Class
1) Attendance
2) Time on the Equipment and Effort
3) Turn in reports on time!!