Specimen Processing
11th Annual Workshop
on
During the hands-on specimen prep portion of the
workshop tissue will be provided. Participants may
choose to work with either neonatal myocardial mouse
fibroblasts grown on coverslips or adult c57 wildtype
mouse heart sections.
Individuals may also choose to bring their own tissue.
Please bring your tissue in PBS after fixation in 4% Paraformaldehyde. Contact Anna Harper for further questions or details.
Basic Confocal
Microscopy
June 13- 17, 2016
Instructors:
Dr. Robert Price, Research Professor, Cell Biology & Anatomy and Director, Instrumentation Resource Facility, University of South Crolina School of Medicine
Presented by:
Instrumentation
resource Facility
The South Carolina EPSCoR/IDeA Program and
the USC School of Medicine Instrumentation
Resource Facility are pleased to announce the
9th annual workshop on Basic Confocal Microscopy. The hands-on workshop will target beginning and intermediate users of confocal microscopes and will provide lectures from experts in the field of confocal microscopy and
the use of Adobe Photoshop and 3-D software
for processing confocal images. Lecture material will provide information on the basics of fluorescence and fluorescent probes, biological
specimen preparation (fixation, staining, optical properties and mounting materials), strategies and protocols for selection of antibody
labeling, the basic components of a confocal
microscope (lasers, dichroic mirrors, microscope objectives, photomultiplier tubes, etc.)
and an overview of some applications of confocal microscopy.
During the laboratory portion of the workshop specimens will be processed for double
and triple labeling and proper selection of user
adjustable parameters to optimize image collection will be addressed and demonstrated. Participants are welcome to process their
own samples or to use samples that will be provided. Several point scanning and spinning disk
confocal systems from various manufacturers
will be available for use so participants will
have ample time for hands on use of the instruments during the workshop.
Dr. Ralph Albrecht, Professor, Department of Animal
Scinces, University of Wisconsin-Madison
Dr. Jay Jerome, Associate Professor, Pathology & Cancer
Biology, Vanderbilt University
Dr. John Mackenzie, Professor, Microbiology, North Carolina State University
Dr. Thomas Trusk, Associate Professor, Cell Biology &
Anatomy, Medical University of South Carolina
Past Participating Representatives:
Leica: TCS, LSI
Nikon: C1 SI
Olympus: Disk Scanner, Fluoview, FV10i
Perkin Elmer: Ultraview
Photometrics: Metamorph
USC School of Medicine
Instrumentation Resource Facility
6439 Garners Ferry Rd.
Building 1 Room B-60
Columbia SC 29209
Phone: 803-216-3825
Fax: 803-216-3847
For more information contact Anna Harper
E-mail: amharper@sc.edu
Monday June 13, 2016
8:30-9:00
Registration and Continental Breakfast
9:00-9:15
Welcome and Logistics
9:15-10:30
Introduction and Overview of Confocal
Microscopy: Price
10:30-11:00
Break, Discussion, Meet with Workshop
staff to arrange specimen processing
11:00-12:00
Specimen Fixation and Processing: Jerome
12:00-12:45
Lunch (Provided) and discussion
12:45-2:00
Antigen: Antibody Interactions, Label
ing Protocols and Strategies: Albrecht
2:00-2:15
Break
2:15-4:15
Antigen: Antibody Interactions, Labeling Protocols and Strategies – continued
4:15-4:30
Organization of Lab Groups and Instructions - Price
4:30-5:30
Lab – Specimen Preparation and Processing: Processing of own samples or
samples will be provided. Fixation
through overnight primary antibody
incubation
8:30-9:00
Continental Breakfast
9:00-10:00
3-D reconstruction of confocal data
sets with AMIRA: Trusk
Lab - Specimen Processing
10:00-10:15
Break
1:30-2:30
Components, operating parameters,
and types of confocal microscopes:
Price
10:15-12:00
3-D reconstruction of confocal data
sets with AMIRA: Continued
12:00–12:45
Lunch and Seminar
2:30- 2:45
Break
12:45-4:30
2:45-4:30
Lab – finish specimen processing
4:30-5:45
Time on Instruments (Leica, Nikon,
Olympus, and Zeiss systems are scheduled to be available)
Additional Time on Confocal Instruments and Software; Time on Computers with Faculty for Image Enhancement and Analysis with Photoshop and
AMIRA
5:45-6:45
Dinner Provided: Dinner Seminar
6:00-??
Reception on the shore of Lake Murray
6:45-9:15
Time on Instruments
11:00-12:00
Digital Images and Resolution: Jerome
12:00-1:00
Lunch (Provided)
1:00-1:30
Continental Breakfast
Wednesday June 15, 2016
9:00-10:00
Ethics in Use of Confocal Images,
Available Resources, Discussion
8:30-9:00
Continental Breakfast
10:00-12:30
9:00-10:30
Components, operating parameters:
Price
Additional Time on Confocal Instruments and Software; Time on Computers with Faculty for Image Enhancement and Analysis with Photoshop and
AMIRA
12:30-1:15
Lunch
1:15-3:45
Additional Time on Confocal Instruments and Software; Time on Computers with Faculty for Image Enhancement and Analysis with Photoshop and
AMIRA
Dinner Provided: Dinner Seminar
10:30-10:45
Break
6:45-8:30
Additional lecture material on specimen preparation, labeling strategies,
etc.: Albrecht
10:45-11:15
Resolution, Digital Images, Image Formats: Jerome
11:15-12:00
Photoshop, etc with confocal images:
Mackenzie
12:00-12:45
Lunch
12:45-2:00
Photoshop, etc with confocal images:
Mackenzie
2:00-2:15
Break
2:15-3:00
Photoshop, etc with confocal images:
Mackenzie
3:00-6:45
Time on Instruments
Tuesday June 14, 2016
Continental Breakfast
9:00-10:00
Lab - Wash specimens and secondary
antibody incubation
10:00-11:00
Basics of Fluorescence, Dye Characteristics: Jerome
Friday June 17, 2016
8:30-9:00
5:45-6:45
8:30-9:00
Thursday June 16, 2016
Tuesday continued…
*Dinner on your own*
Schedule may be altered prior to June 2016
please check our website for updates.
http://irf.med.sc.edu/