Genetics and Heredity Chapter 8 Mendel Studied 7 Characters in the Garden Pea (Pisum sativum) • • • • • • • Flower color. Flower position. Seed color. Seed shape. Pod color. Pod shape. Stem length. Mendel’s Methods • Cross-pollination- the transfer of pollen from one plant to the stigma of another. – Self-pollination – Artificial cross-pollination • Monohybrid crosses- an experimental cross between individuals that differ by a single trait. • First (F1) and second (F2) generations. Mendel’s F1 Observations • One of the traits could be seen in the F1 generation= Dominant • One of the traits could not be seen in the F1 generation= Recessive 100% Purple Genetic Terminology • Phenotype- an organisms traits. • Genotype- an organisms genetic makeup. • Homozygous- identical alleles for a gene. • Heterozygous- two different alleles for a gene. Genetic Terminology #2 • Allele- one of 2 or more alternative forms of the same gene. • Gene- a sequence of DNA that codes for a protein. • Locus- the position on a chromosome occupied by a gene. W w Mendel’s F2 Observations • Flower color – Purple:white 705:224 3:1 Mendel’s Dihybrid Cross • 315:108:101:32 = 9:3:3:1 Mendel’s Work Yielded These Genetic Rules • Alternative versions of genes (different alleles) account for variations in inherited characters. • For each character an organism inherits two alleles, one from each parent. • If two alleles differ, then one, the dominant allele is fully expressed in the organism’s appearance. • The two alleles for each character segregate during gamete production. • Alleles of a gene segregate independently of the alleles of other genes. Punnett Squares and the Testcross • Punnett square- a diagram used to predict the result of a genetic cross. • Testcross- a genetic experiment used to determine an organisms genotype. ? w w W Ww Ww w ww ww ? w w W Ww Ww W Ww Ww Incomplete Dominance • Incomplete dominance- type of inheritance in which the F1 is intermediate in phenotype between the parents. • Neither allele is dominant. • Self-pollination of the F1 yields a 1:2:1 F2 population. Continuous Variation • Continuous variation- a gradation in phenotype; indicates that a trait is controlled by two or more genes. – Polygenic inheritance. Linkage • Linkage- the tendency for certain genes to be inherited together, owing to the fact that they are located on the same chromosome. • Linked genes- genes that are inherited together. Genetically Engineered Rice Genetically Modified Rice Genetic Engineering • Genetic Engineering- the technique of removing, modifying, or adding genes to a DNA molecule. – Improvements in crops • Longevity • Resistance to insects • Resistance to herbicides Transgenic Plants • Tomato with BT gene and without. • Transgenic tomatoes with and without etr1-1 gene. • Petunia with and without etr1-1 gene. • Tobacco with and without a cytokinin gene. Transfer of Genes Agrobacterium tumefaciens Genomics • Genomics- a field of genetics that attempts to understand the content, organization, function, and evolution of genetic information in a whole organism. Arabidopsis thaliana Genome • Genome- all of the genetic information of an organism.