Do Now Cell membranes are composed of proteins and a type of lipid called________________. Phospholipids only have 2 ________ ________ chains. Is the phospholipid bilayer rigid or fluid-like? I. The Cell Builds a Diversity of Products - - - OBJECTIVES Identify the role of the nucleus Describe how the functions of ribosomes, endoplasmic reticulum, and the Golgi apparatus are related. Distinguish between the functions of vacuoles and lysosomes Summarize the path of cellular products through the membrane. A. Structure and Function of the Nucleus • The nucleus of a eukaryotic cell contains most of the cell’s DNA. • The information stored in DNA directs the activities of the cell. • DNA is attached to proteins, forming long fibers called chromatin. Nucleus • Nuclear envelope- surrounds the nucleus • Nuclear pores- openings where substances move from the nucleus into the cytoplasm • Nucleolus- a dense mass within the nucleus where ribosomes are made. B. Ribosomes • Ribosomes- clusters of proteins and nucleic acids. • Ribosomes are responsible for making proteins. • Ribosomes are either attached to endoplasmic reticulum or float around in the cytoplasm. Ribosomes attached to E.R. C. Assembly and Transportation • Endoplasmic Reticulum~ the site of cellular chemical reactions. • manufactures and transports molecules within a cell. - Rough E.R.- studded with ribosomes. - makes proteins - Smooth E.R.- lacks ribosomes - makes and stores lipids 1. Rough E.R. • The ribosomes produce – proteins that are inserted into the membrane – proteins that are packaged in vesicles and exported by the cell. • Example: Salivary glands secrete a lot of protein (enzymes) into your mouth… so these cells contain a lot of Rough E.R. 2. Smooth E.R. • This ribosome-free portion of the membrane • contains enzymes that perform various functions like building lipids • Example: Ovaries and Testes produce sex hormones (lipids-cholesterol)…so these cells contain a lot of smooth E.R. Endoplasmic Reticulum D. The Golgi Apparatus • Proteins leave the Rough E.R. – Transported in vesicles to the: • Golgi apparatus~ modifies, stores, and ships proteins and other molecules to their final destination. E. Vacuoles & Storage • Vacuoles~ membrane-bound sacs Temporary storage of undigested nutrients, salt, water, pigments, waste, poisons. Plant Vacuole F. Lysosomes & Recycling • Lysosomes~ membrane-bound sacs that contain digestive enzymes – Break down • worn out organelles • food particles • viruses and bacteria Membrane Pathways in a Cell DO NOW 1. 2. 3. 4. What is the structural difference between rough and smooth endoplasmic reticulum? Where can you find most of the cell’s DNA? What organelle is responsible for modifying, storing, and shipping proteins? What organelle contains enzymes for breaking down food and bacteria? II. Chloroplasts and Mitochondria OBJECTIVE: COMPARE AND CONTRAST THE FUNCTIONS OF CHLOROPLASTS AND MITOCHONDRIA A. Chloroplasts~ – found only in plant cells and some protists – capture light energy and convert it to chemical energy through photosynthesis • Plastids~ – plant organelles that are used for storage of starches, lipids, or pigments. – Example: chloroplast • Chlorophyll~ – green pigment located in thylakoid discs of chloroplast – traps light energy – Granum~ stack of thylakoids – Stroma~ fluid surrounding the granum Light-absorbing chlorophyll molecules are present in thylakoid discs that fill the chloroplast. B. Mitochondria- “powerhouse” – (found in plant and animal cells) – site of cellular respiration – converts energy so that it is useful for powering cellular work Mitochondria are the powerhouses of the cell. They take in fuel molecules derived from sugars and fats, harvest the energy in their chemical bonds with the aid of oxygen, and spit out ATP. III. An internal skeleton supports the cell and enables movement OBJECTIVES: Describe the roles of the cytoskeleton in cell movement Compare and contrast the functions of flagella and cilia Explain why a cell can be described as a coordinated unit INTRODUCTION A cell has its own internal support system that enables it to move, support organelles, and maintain shape. A. Cytoskeleton~ – a network of protein fibers extending throughout the cytoplasm. • Two types of fibers: – Microtubules- straight, hollow tubes – Give rigidity and organization to cell – EX: movement of lysosomes to reach & fuse with a food vacuole – Ex: centrioles » Important for cell division – Microfilaments- thinner, solid rods – Allow cells to move & change shape – Ex: movement of amoebas – Ex: muscle fibers » Involved in muscle contraction B. Organelles for Movement 1. Flagella- long, thin, whip-like structures that enable cells to move – EX: sperm • (S-shaped motion) 2. Cilia- shorter & more numerous than flagella – Ex: cells lining your windpipe • cilia sweep mucus and trapped debris out of your lungs • (back-and-forth motion) Sperm Cells with Flagella Bronchioles lined with cilia The Cell as a Coordinated Unit A cell is a living unit that is greater than the sum of its parts. A cell can only work when all of its organelles work together. A white blood cell defends the body against infections by ingesting bacteria 1. 2. 3. 4. 5. 6. WBC moves toward bacteria using cytoplasmic extensions WBC engulfs bacteria Bacteria is destroyed by lysosomes that were produced by E.R. and Golgi apparatus Ribosomes made the proteins of the cytoskeleton and the enzymes inside the lysosomes The production of these proteins was programmed by messages dispatched from DNA in nucleus All these processes require ATP, which is supplied by mitochondria