Analyzing Data from an Experiment Now that I have my experimental data, how do I begin to understand them? Contents I. Once I have my experimental data, how do I begin to understand them? ................................................. 2 II. What type of data do I have? .................................................................................................................... 3 Discrete data: ........................................................................................................................................... 3 Continuous data: ...................................................................................................................................... 3 Categorical data: ...................................................................................................................................... 3 Normal Data ............................................................................................................................................. 4 III. Is my data paired or unpaired? ................................................................................................................ 5 IV. Do I want to compare my values to the mean or to the standard deviation? .......................................... 6 V. Testing a Hypothesis ................................................................................................................................ 7 Why p < 0.05 ? .......................................................................................................................................... 8 VI. Using an Unpaired T-Test....................................................................................................................... 9 VII. Using a Chi-Square Test for Variance................................................................................................. 13 VIII. Using a Paired T-Test ........................................................................................................................ 15 IX. Using a Chi-Square Test for Goodness-of-Fit ...................................................................................... 17 Appendix 1: Critical Values for a T-Test.................................................................................................... 19 Appendix 2: Critical Values for a Chi-Square Test .................................................................................... 20 Created by the HHMI-FT team at the University of North Carolina at Chapel Hill, 2013. Analyzing Data from an Experiment I. Once I have my experimental data, how do I begin to understand them? The point of analyzing any quantitative data that you collect in an experiment is to see if it does or does not support your original hypothesis. One way to determine whether or not this happens is to use a statistical test, which can tell you whether or not your data are statistically significant. There are many different types of data that you can collect in your experiment, and each type has its own statistical test to determine significance. However, it can sometimes be difficult to figure out exactly what test to use, and exactly how to use that particular test. Follow this flow chart and use the resources below to match the type of data you have to the appropriate statistical test! Page 2 of 22 Analyzing Data from an Experiment II. What type of data do I have? Data can be collected in a variety of forms. When data are presented as numbers (known as numerical or quantitative data), instead of words (known as categorical, descriptive, or qualitative data), they can be either continuous or discrete. Discrete data: This is in contrast with continuous data. If your data is discrete, the statistical tests on this page are not appropriate for your calculations. Discrete data can be counted. It is made of numbers that cannot be split up into an infinite amount of values. o Some examples of variables that would be discrete are shoe sizes or the counts of an item (number of tomatoes on a vine, number of people in a room). Something like a size 9.452 shoe or 12.8516 tomatoes is not a meaningful piece of data. Continuous data: Continuous data Continuous data is data that can be measured. It has an infinite number of points that exist within a range of values, and any of the values have a specific meaning. For example, consider 0 to 100, -17.5 to 9371, or -infinity to +infinity as possible ranges. o In the range of 0 to 100, you can get whole numbers like 1, 2, 3, or 4, but you can also get an unlimited amount of values between each whole number, like 1.1, 1.001, 1.0001, and so on. If you were to visualize a set of continuous data, you would be able to draw it on a graph without picking up your pencil. Some examples of data sets that would be continuous: o growth measures (height, finger length, foot width) o things that depend on time (acceleration of a car, walking speed) o distances (how far each person walks on an average day) Continuous data can be represented on a coordinate plane or scatterplot. Some examples of how a continuous graph looks: On the other hand, when data is presented with words that can be described in numbers, it is categorical data. Categorical data: Page 3 of 22 Analyzing Data from an Experiment Some examples of data that would be categorical: o types of music played on the radio and how many stations play each type o the number of each species of animal in an area o the number of hotels in each state of the US. Categorical data can be represented on a bar graph, pie chart, or a dot chart (a chart that shows the percentage of each category in the data). The data is split into different categories (hence the name!) and the numbers describe each category. Some examples of how a graph of categorical data looks: Normal Data Many statistical tests are valid only if the population (the body from which measurements were taken) has a normal distribution. For example, if a study was done on the heights of kindergarteners in a class, the population would be all kindergarteners in general. Normally distributed populations have certain measures associated with symbols that are commonly used in statistical calculations. A normal distribution is data that looks like a bell curve when plotted on a density curve (the independent axis showing the values of the data points and the dependent axis showing the frequency of those values). The bell curve is symmetric and can describe many bodies of data, with a majority of the subjects in the middle range and fewer and fewer subjects in the lower and higher ranges. A normal distribution looks like this: Page 4 of 22 Analyzing Data from an Experiment III. Is my data paired or unpaired? Paired data is data in a study that includes two sets of measurements about the same observed subject. Some general situations involving paired data: before and after (the height of a group of plants plant before applying a fertilizer and the height of the same group of plants after applying fertilizer) comparing closely related things (weight gained over a span of time in sets of twins, the number of nights experiencing insomnia in married couples) The body of subjects in a set of paired data is called a matched population, because each subject in the population is matched with another subject. Independent populations, on the other hand, contain data that do not affect each other. Two or more sets of measurements are not influenced in any way by each other. These measurements would be unpaired. Some situations involving unpaired data: comparing a sample to an expected standard (average temperature of a city for 100 years compared to data taken over the last two years) comparing a treatment group to a control group (sleep time of a group treated by a sleeping pill compared to sleep time of a group not treated by a sleeping pill) Page 5 of 22 Analyzing Data from an Experiment IV. Do I want to compare my values to the mean or to the standard deviation? A population includes all possible objects of interest, whereas a sample includes only a portion of the population. For example: in a study of United States middle school students, the population is every middle school student in the United States and a sample would be the middle schoolers in one school. The mean (represented by μ ("mu") for a population and x ("x-bar") for a sample) is calculated like any arithmetic mean – by adding all of the values and dividing it by the total number of values. The standard deviation (represented by σ ("sigma") for a population, and s for a sample) or sample standard deviation (s) is a number that describes the spread of the numbers in a set of data, or how far apart the numbers are. The larger the value of the standard deviation, the more spread out the numbers. It is calculated by taking the square root of the average distance that each point is from the mean. Standard deviation is calculated using these formulas: The degrees of freedom (df) in a statistical study is one less than the total number of subjects. For instance, if 100 people were surveyed in a study, the degrees of freedom would be 99. Page 6 of 22 Analyzing Data from an Experiment V. Testing a Hypothesis In performing statistical analysis, hypotheses must first be designated for determining statistical significance. The null hypothesis (represented by h0) is the statement being tested and usually assumes that the expected effect in a study is NOT true. The alternate hypothesis (represented by h1) is the statement of the expected effect and is accepted if the null hypothesis can be rejected based on the statistical calculations. For instance, in determining whether there is a significant difference in the average grades of one class versus another class, the hypotheses would look like this: Null hypothesis (h0) : There is no difference in the average grades of the two classes. Alternate hypothesis (h1) : There is a difference in the average grades of the two classes. An alpha value (α) is used to determine what qualifies as statistically significant. The alpha value is the percentage likelihood that the difference found in the calculations is due to chance. To determine the alpha value for a given statistical test, you must compare your statistic to a table of critical values. For example, for a t-test, you would compare your t-statistic, given your specific degrees of freedom, to find your alpha value. A table of critical values for T-tests can be found on page 17. A table of critical values for Chi-Square tests can be found on page 18. Page 7 of 22 Analyzing Data from an Experiment Why p < 0.05 ? An alpha value (α) is set at a certain value to determine what qualifies as statistically significant. The p value is the percentage likelihood that the difference found in the calculations is due to chance. The scientifically accepted alpha value for statistical significance is 0.05, which says that there is a 0.05 or 5% likelihood that the data is due to chance. So if you were to find a p value lower than 0.05, your calculations would show the research is statistically significant. In testing for the validity of an alternate hypothesis, you need to decide whether to use a one- or two-tailed test. A one-tailed test determines whether the sample in question is either statistically higher than an expected value or statistically lower than an expected value. The test only determines whether it is ONE of these extremes and does not account for statistical differences in the other extreme. A two-tailed test determines whether the sample in question is either statistically higher OR lower than an expected value. The test determines whether it is either one of these extremes in the same test. If you find that your results are statistically significant, then you can reject the null hypothesis and accept the alternate hypothesis. If you do not find that your results are statistically significant, then you do not have a conclusive statistical analysis. Page 8 of 22 Analyzing Data from an Experiment VI. Using an Unpaired T-Test One Hypothesis Null Hypothesis: The sample mean is equal to the population mean. How the test works: The test statistic is calculated using a formula that has the difference between the means in the numerator; this makes the test statistic get larger as the means get further apart. The denominator is the standard error of the difference in the means, which gets smaller as the sample variances decrease or the sample sizes increase. Thus the test statistic gets larger as the means get farther apart, the variances get smaller, or the sample sizes increase. The probability of getting the observed test statistic value under the null hypothesis is calculated using the t-distribution. The shape of the t-distribution, and thus the probability of getting a particular test statistic value, depends on the number of degrees of freedom. The degrees of freedom for a t-test is the total number of observations in the groups minus 2, or n1+n2-2. Assumptions: The t-test assumes that the observations within each group are normally distributed and the variances are equal in the two groups. Example: In the past semesters for the last 5 years, students in the 5 p.m. section of my Biology class had an average height of 64.6 inches. This year there were 5 students with the following heights. Is the average heights of the previous sections significantly different from their heights? Here is the data: 5 p.m. 68 62 67 68 69 1. Determine the mean. Mean: (68 + 62 + 67 + 68 + 69) / 5 = 66.8 2. Determine the standard deviation. ∑(𝑥 − 𝑥̅ )2 = (68 − 66.8)2 + (62 − 66.8)2 + (65 − 66.8)2 + (68 − 66.8)2 + (69 − 66.8)2 Page 9 of 22 Analyzing Data from an Experiment ∑(𝑥 − 𝑥̅ )2 = 30.8 30.8 30.8 𝑠= √ = √ = √7.7 = 2.775 5−1 4 3. Calculate the t-statistic. 𝑡= (𝑥̅ − 𝜇) (66.8 − 64.6) = = 1.773 𝑠 2.775 5 √𝑛 4. Find the degrees of freedom. df = n - 1 = 4 5. Use the degrees of freedom and your t-statistic to determine your p-value based on a chart of critical values. According to this table, 0.05 < p < 0.010, and thus the null hypothesis can NOT be rejected. Two Populations Unpaired t-test When to use: An unpaired t-test is used when you are comparing the means for two different groups or populations. It is usually used when you are comparing the mean from an experimental group to the mean of a control group. So the individuals in group 1 are not the same individuals in group 2. How to do an unpaired t-test: Suppose you expose caterpillars to different temperatures (25°C and 33°C) to see if they develop into the next instar (stage) faster at the higher 33°C temperature. 25°C Caterpillars (Control) Days 33°C Caterpillars (Experimental) Days Cat. 1 2 Cat. 4 3 Cat. 2 4 Cat. 5 2 Cat. 3 3 Cat. 6 3 Page 10 of 22 Analyzing Data from an Experiment 1. First identify null and alternative hypotheses: Null hypothesis: The mean time for a caterpillar to develop into the next instar is the same between the control and experimental group. Alternative hypothesis: The mean time for a caterpillar to develop into the next instar is faster in the experimental group than in the control group. 2. Now calculate the means for each group: Control group: (2+4+3)/3 = 3 Experimental group: (3+2+3)/3 = 2.67 3. Now add the squares of the group mean minus each data value for both the control and experimental group: (control x-control mean)2 = (2-3)2 + (4-3)2 + (3-3)2 = 2 (experimental y- experimental mean)2 = (3-2.67)2 + (2-2.67)2 + (3-2.67)2 = 0.667 4. Now Calculate variance: So you can set it up like below (*note use your calculations from above to fill in the equation): S2= (control x-control mean)2 + (Exp. 33°C y-Exp. 33°C mean)2 n1+ n2 – 2 S2= 2 + 0.667 3+3-2 S2 = 0.667 5. Now perform an unpaired t-test: t= 3.0 - 2.67 = 0.495 √(0.667((1/3)+ (1/3)) 6. Now look at the table of critical values of t-distributions and determine the range the p-value must fall in (p<0.05 being significant) (degree of freedom= n1+n2 – 2). Degree of freedom = 3+3-2= 4 Page 11 of 22 Analyzing Data from an Experiment p>0.25 The means between the control and experimental group were NOT significantly different therefore; you cannot reject the null hypothesis. Page 12 of 22 Analyzing Data from an Experiment VII. Using a Chi-Square Test for Variance When to do this test? This test is useful if you have data that follows a normal distribution. This test compares the data to the standard deviation. To use this test you need continuous data. What is the equation? Where: o o o n = the total number of data s = sample variance (standard deviation) σ = population variance (known variance) What does it tell you? The chi-squared test for variance is similar to the chi squared test for goodness of fit. You still have a null hypothesis and an alternative hypothesis. This test will tell you if the variance in a sample matches the hypothesized variance. When you complete the chi-square test for variance on a data set, you will compare your final chi-square value and your degrees of freedom to a chart of critical values. Your critical value will then determine whether or not you can reject your null hypothesis. A table of critical values for the chi-square test can be found on page 18. Why is it useful? Imagine you work in a factory and know that the boxes get filled accurately to 50 pounds with an average standard deviation of about 1 pound. You hire a new set of packers and want to make sure they are as accurate as the last. You would use the chi-squared test for variance to compare the standard deviation of the current packers to the known average standard deviation of the factory. Overall, this test compares a set of data to the standard deviation and reveals the variance in the data set compared to the expected variance in the data set. Example: A cereal manufacturer wishes to test the claim that the variance of sugar content of its cereals is 0.644. Sugar content is measured in grams and is assumed normally distributed. A sample of 20 cereals has a standard deviation of 1.00 gram. At α = 0.05, is there enough evidence to reject the manufacturer’s claim? 1. State the hypotheses and identify the claim. 2. Find the critical values. Since this test is a two-tailed test at α = 0.05, the critical values for 0.025 and 0.975 must be found. The degrees of freedom are 19; hence, the critical values are 32.852 and 8.907, Page 13 of 22 Analyzing Data from an Experiment respectively. Find this using a chi squared table. A table showing critical values for a chisquare test can be found on page 17. 3. Compute the test value. Since the standard deviation s is given in the problem, it must be squared for the formula. 4. Make the decision. Do not reject the null hypothesis, since the test value falls between the critical values region. 5. Summarize the results. There is not enough evidence to reject the manufacturer’s claim that the variance of the sugar content of the cereals is equal to 0.644. Page 14 of 22 Analyzing Data from an Experiment VIII. Using a Paired T-Test Purpose: The paired t-test usually compares the mean of a population before and after a treatment. For a paired t-test, you must take the mean of the difference between the two pairs (before and after). This test assumes that the difference between pairs is normally distributed. How to do a paired t-test: Suppose you take the blood pressure of three sixth graders before they eat a piece of cake and again 30 minutes after they eat the cake and get the following: Sixth Grader Student 1 Student 2 Student 3 Blood Pressure Before Eating Cake 115 122 118 Blood Pressure After Eating Cake 117 123 121 You want to know if their blood pressure is significantly higher after they eat the cake than before, so you decide to use a paired t-test. 1. First you must find the differences: Student 1: 117 - 115 = 2 Student 2: 123 - 122 = 1 Student 3: 121 - 118 = 3 2. Now calculate the mean: 2+1+3=6 6/3=2 3. What is the null hypothesis? The null hypothesis assumes that the mean difference between the pairs is zero. So H0 = 0, which in the equation is subtracted from the mean difference you calculated in step 2. 4. What is the alternative hypothesis? The alternative hypothesis assumes that the mean difference in blood pressure will be higher after eating the cake. Therefore, the alternative hypothesis would be H1: μ > 0. 5. Now let’s calculate standard deviation: s = (2-2)2 + (1-2)2 + (3-2)2 = 2 s = 2/ (3-1) = 1 Page 15 of 22 Analyzing Data from an Experiment 6. Now we can do our paired t-test: t = 1.73 7. What are our degrees of freedom? Because df = n - 1, our degrees of freedom will be 2 for this example. 8. Find our p-value. Now we want to use our t-statistic and our degrees of freedom to find our p-value from a chart of critical values. A chart of critical values for the T-test can be found on page 17. Based on this chart, we find that: 0.10 < p < 0.15 9. Can our null hypothesis be rejected? If we consider p < .05 to be statistically significant, we can NOT conclude that the mean difference in blood pressure for these students before and after eating a piece of cake is statistically significant. Therefore, we cannot assume that blood pressure is higher after eating a piece of cake. Page 16 of 22 Analyzing Data from an Experiment IX. Using a Chi-Square Test for Goodness-of-Fit A Chi Square Goodness of Fit test can be used when you have categorical data (ex: males and females or heads and tails of a coin). After completing an experiment, you may want to determine whether your observed results match your expected results (hypothesis). A Chi Square Goodness of Fit Test will allow you to determine this. Performing a Chi Square Goodness of Fit Test: 1. Specify a null hypothesis. The null hypothesis states that there is no significant difference between observed and expected. In this scenario the probability is greater than .05 (p > .05) 2. Specify an alternative hypothesis. The alternative hypothesis states that there is a significant difference between the observed and expected. However, this test only tells you that there is a difference. It does not tell you what the difference is. In this scenario, the probability is less than .05 (p < .05). o What does this mean? Imagine you are flipping a coin 100 times. You want to know how many times you land on heads and tails. You expect that you will land on heads 50 times and tails 50 times. These are your expected results. However, after performing the experiment, you land on heads 43 times and tails 57 times. These are you observed results. You can visualize in a table like this: Heads Tails 50 Expected 50 57 Observed 43 3. Now it is time to do the math. X2 = [ (43-50)2 / 50] + [ (57-50)2 / 50 ] X2 = 0.98 + 0.98 = 1.96 Now that we know the chi square value is 1.96, we will move on to the chi square table. 4. Finding degrees of freedom: Degrees of freedom is a fancy term for number of categories minus 1. In this experiment we have two categories (heads and tails), so df = 2 – 1. 5. Reading the chi square table: o A table showing critical values for a chi-square test can be found on page 18. o Degrees of Freedom are listed down the left side of the table. Find the degrees of freedom that corresponds to your sample. In this case df = 1. Page 17 of 22 Analyzing Data from an Experiment o o Now, you go across the table until you come across you chi-square value. In this case X2 = 1.96. Once you have found this value, go up to the top of the table and find the alpha value. According to the table, our alpha value is 0.15. 6. Testing Significance: You must now compare the alpha value and the p-value of 0.05. 0.15 > 0.05 7. Conclusion: Refer back to what the null and alternative hypotheses mean. Because 0.15 is greater than 0.5 we can say that there is no significant difference between the observed and the expected. In other words, we fail to reject the null hypothesis. (However, you cannot state that the null hypothesis was proven because in science it is very hard to prove something is 100% true all the time.) Additionally, we reject the alternative hypothesis. This means that this test shows there is not a significant difference between 57:43 (observed) and 50:50 (expected). Therefore, these results were due to chance and not an underlying factor (such as a “trick” coin). 8. Going a step further: If the alpha value had been 0.03, would we have rejected or failed to reject the null hypothesis? Rejected. Page 18 of 22 Analyzing Data from an Experiment Appendix 1: Critical Values for a T-Test 1 tail α = 2 tails α = df =1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 60 120 ∞ 0.1 0.2 3.078 1.886 1.638 1.533 1.476 1.440 1.415 1.397 1.383 1.372 1.363 1.356 1.350 1.345 1.341 1.337 1.333 1.330 1.328 1.325 1.323 1.321 1.319 1.318 1.316 1.315 1.314 1.313 1.311 1.310 1.296 1.289 1.282 0.05 0.1 6.314 2.920 2.353 2.132 2.015 1.943 1.895 1.860 1.833 1.812 1.796 1.782 1.771 1.761 1.753 1.746 1.740 1.734 1.729 1.725 1.721 1.717 1.714 1.711 1.708 1.706 1.703 1.701 1.699 1.697 1.671 1.658 1.645 0.025 0.05 12.706 4.303 3.182 2.776 2.571 2.447 2.365 2.306 2.262 2.228 2.201 2.179 2.160 2.145 2.131 2.120 2.110 2.101 2.093 2.086 2.080 2.074 2.069 2.064 2.060 2.056 2.052 2.048 2.045 2.042 2.000 1.980 1.960 0.01 0.02 31.821 6.965 4.541 3.747 3.365 3.143 2.998 2.896 2.821 2.764 2.718 2.681 2.650 2.624 2.602 2.583 2.567 2.552 2.539 2.528 2.518 2.508 2.500 2.492 2.485 2.479 2.473 2.467 2.462 2.457 2.390 2.358 2.326 0.005 0.01 63.656 9.925 5.841 4.604 4.032 3.707 3.499 3.355 3.250 3.169 3.106 3.055 3.012 2.977 2.947 2.921 2.898 2.878 2.861 2.845 2.831 2.819 2.807 2.797 2.787 2.779 2.771 2.763 2.756 2.750 2.660 2.617 2.576 *Taken from http://www.statisticsmentor.com/tables/table_t.htm Page 19 of 22 Analyzing Data from an Experiment Appendix 2: Critical Values for a Chi-Square Test DF 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 0.995 0.000039 0.0100 0.0717 0.207 0.412 0.676 0.989 1.344 1.735 2.156 2.603 3.074 3.565 4.075 4.601 5.142 5.697 6.265 6.844 7.434 8.034 8.643 9.260 9.886 10.520 11.160 11.808 12.461 13.121 13.787 14.458 15.134 15.815 16.501 17.192 17.887 18.586 19.289 19.996 20.707 21.421 22.138 22.859 23.584 24.311 25.041 25.775 26.511 27.249 27.991 28.735 29.481 30.230 30.981 31.735 0.975 0.00098 0.0506 0.216 0.484 0.831 1.237 1.690 2.180 2.700 3.247 3.816 4.404 5.009 5.629 6.262 6.908 7.564 8.231 8.907 9.591 10.283 10.982 11.689 12.401 13.120 13.844 14.573 15.308 16.047 16.791 17.539 18.291 19.047 19.806 20.569 21.336 22.106 22.878 23.654 24.433 25.215 25.999 26.785 27.575 28.366 29.160 29.956 30.755 31.555 32.357 33.162 33.968 34.776 35.586 36.398 0.20 1.642 3.219 4.642 5.989 7.289 8.558 9.803 11.030 12.242 13.442 14.631 15.812 16.985 18.151 19.311 20.465 21.615 22.760 23.900 25.038 26.171 27.301 28.429 29.553 30.675 31.795 32.912 34.027 35.139 36.250 37.359 38.466 39.572 40.676 41.778 42.879 43.978 45.076 46.173 47.269 48.363 49.456 50.548 51.639 52.729 53.818 54.906 55.993 57.079 58.164 59.248 60.332 61.414 62.496 63.577 0.10 2.706 4.605 6.251 7.779 9.236 10.645 12.017 13.362 14.684 15.987 17.275 18.549 19.812 21.064 22.307 23.542 24.769 25.989 27.204 28.412 29.615 30.813 32.007 33.196 34.382 35.563 36.741 37.916 39.087 40.256 41.422 42.585 43.745 44.903 46.059 47.212 48.363 49.513 50.660 51.805 52.949 54.090 55.230 56.369 57.505 58.641 59.774 60.907 62.038 63.167 64.295 65.422 66.548 67.673 68.796 0.05 3.841 5.991 7.815 9.488 11.070 12.592 14.067 15.507 16.919 18.307 19.675 21.026 22.362 23.685 24.996 26.296 27.587 28.869 30.144 31.410 32.671 33.924 35.172 36.415 37.652 38.885 40.113 41.337 42.557 43.773 44.985 46.194 47.400 48.602 49.802 50.998 52.192 53.384 54.572 55.758 56.942 58.124 59.304 60.481 61.656 62.830 64.001 65.171 66.339 67.505 68.669 69.832 70.993 72.153 73.311 0.025 5.024 7.378 9.348 11.143 12.833 14.449 16.013 17.535 19.023 20.483 21.920 23.337 24.736 26.119 27.488 28.845 30.191 31.526 32.852 34.170 35.479 36.781 38.076 39.364 40.646 41.923 43.195 44.461 45.722 46.979 48.232 49.480 50.725 51.966 53.203 54.437 55.668 56.896 58.120 59.342 60.561 61.777 62.990 64.201 65.410 66.617 67.821 69.023 70.222 71.420 72.616 73.810 75.002 76.192 77.380 0.02 5.412 7.824 9.837 11.668 13.388 15.033 16.622 18.168 19.679 21.161 22.618 24.054 25.472 26.873 28.259 29.633 30.995 32.346 33.687 35.020 36.343 37.659 38.968 40.270 41.566 42.856 44.140 45.419 46.693 47.962 49.226 50.487 51.743 52.995 54.244 55.489 56.730 57.969 59.204 60.436 61.665 62.892 64.116 65.337 66.555 67.771 68.985 70.197 71.406 72.613 73.818 75.021 76.223 77.422 78.619 0.01 6.635 9.210 11.345 13.277 15.086 16.812 18.475 20.090 21.666 23.209 24.725 26.217 27.688 29.141 30.578 32.000 33.409 34.805 36.191 37.566 38.932 40.289 41.638 42.980 44.314 45.642 46.963 48.278 49.588 50.892 52.191 53.486 54.776 56.061 57.342 58.619 59.893 61.162 62.428 63.691 64.950 66.206 67.459 68.710 69.957 71.201 72.443 73.683 74.919 76.154 77.386 78.616 79.843 81.069 82.292 0.005 7.879 10.597 12.838 14.860 16.750 18.548 20.278 21.955 23.589 25.188 26.757 28.300 29.819 31.319 32.801 34.267 35.718 37.156 38.582 39.997 41.401 42.796 44.181 45.559 46.928 48.290 49.645 50.993 52.336 53.672 55.003 56.328 57.648 58.964 60.275 61.581 62.883 64.181 65.476 66.766 68.053 69.336 70.616 71.893 73.166 74.437 75.704 76.969 78.231 79.490 80.747 82.001 83.253 84.502 85.749 0.002 9.550 12.429 14.796 16.924 18.907 20.791 22.601 24.352 26.056 27.722 29.354 30.957 32.535 34.091 35.628 37.146 38.648 40.136 41.610 43.072 44.522 45.962 47.391 48.812 50.223 51.627 53.023 54.411 55.792 57.167 58.536 59.899 61.256 62.608 63.955 65.296 66.633 67.966 69.294 70.618 71.938 73.254 74.566 75.874 77.179 78.481 79.780 81.075 82.367 83.657 84.943 86.227 87.507 88.786 90.061 0.001 10.828 13.816 16.266 18.467 20.515 22.458 24.322 26.124 27.877 29.588 31.264 32.909 34.528 36.123 37.697 39.252 40.790 42.312 43.820 45.315 46.797 48.268 49.728 51.179 52.620 54.052 55.476 56.892 58.301 59.703 61.098 62.487 63.870 65.247 66.619 67.985 69.346 70.703 72.055 73.402 74.745 76.084 77.419 78.750 80.077 81.400 82.720 84.037 85.351 86.661 87.968 89.272 90.573 91.872 93.168 Page 20 of 22 Analyzing Data from an Experiment 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 32.490 33.248 34.008 34.770 35.534 36.301 37.068 37.838 38.610 39.383 40.158 40.935 41.713 42.494 43.275 44.058 44.843 45.629 46.417 47.206 47.997 48.788 49.582 50.376 51.172 51.969 52.767 53.567 54.368 55.170 55.973 56.777 57.582 58.389 59.196 60.005 60.815 61.625 62.437 63.250 64.063 64.878 65.694 66.510 67.328 68.146 68.965 69.785 70.606 71.428 72.251 73.075 73.899 74.724 75.550 76.377 77.204 78.033 78.862 37.212 38.027 38.844 39.662 40.482 41.303 42.126 42.950 43.776 44.603 45.431 46.261 47.092 47.924 48.758 49.592 50.428 51.265 52.103 52.942 53.782 54.623 55.466 56.309 57.153 57.998 58.845 59.692 60.540 61.389 62.239 63.089 63.941 64.793 65.647 66.501 67.356 68.211 69.068 69.925 70.783 71.642 72.501 73.361 74.222 75.083 75.946 76.809 77.672 78.536 79.401 80.267 81.133 82.000 82.867 83.735 84.604 85.473 86.342 64.658 65.737 66.816 67.894 68.972 70.049 71.125 72.201 73.276 74.351 75.424 76.498 77.571 78.643 79.715 80.786 81.857 82.927 83.997 85.066 86.135 87.203 88.271 89.338 90.405 91.472 92.538 93.604 94.669 95.734 96.799 97.863 98.927 99.991 101.054 102.117 103.179 104.241 105.303 106.364 107.425 108.486 109.547 110.607 111.667 112.726 113.786 114.845 115.903 116.962 118.020 119.078 120.135 121.192 122.250 123.306 124.363 125.419 126.475 69.919 71.040 72.160 73.279 74.397 75.514 76.630 77.745 78.860 79.973 81.085 82.197 83.308 84.418 85.527 86.635 87.743 88.850 89.956 91.061 92.166 93.270 94.374 95.476 96.578 97.680 98.780 99.880 100.980 102.079 103.177 104.275 105.372 106.469 107.565 108.661 109.756 110.850 111.944 113.038 114.131 115.223 116.315 117.407 118.498 119.589 120.679 121.769 122.858 123.947 125.035 126.123 127.211 128.298 129.385 130.472 131.558 132.643 133.729 74.468 75.624 76.778 77.931 79.082 80.232 81.381 82.529 83.675 84.821 85.965 87.108 88.250 89.391 90.531 91.670 92.808 93.945 95.081 96.217 97.351 98.484 99.617 100.749 101.879 103.010 104.139 105.267 106.395 107.522 108.648 109.773 110.898 112.022 113.145 114.268 115.390 116.511 117.632 118.752 119.871 120.990 122.108 123.225 124.342 125.458 126.574 127.689 128.804 129.918 131.031 132.144 133.257 134.369 135.480 136.591 137.701 138.811 139.921 78.567 79.752 80.936 82.117 83.298 84.476 85.654 86.830 88.004 89.177 90.349 91.519 92.689 93.856 95.023 96.189 97.353 98.516 99.678 100.839 101.999 103.158 104.316 105.473 106.629 107.783 108.937 110.090 111.242 112.393 113.544 114.693 115.841 116.989 118.136 119.282 120.427 121.571 122.715 123.858 125.000 126.141 127.282 128.422 129.561 130.700 131.838 132.975 134.111 135.247 136.382 137.517 138.651 139.784 140.917 142.049 143.180 144.311 145.441 79.815 81.009 82.201 83.391 84.580 85.767 86.953 88.137 89.320 90.501 91.681 92.860 94.037 95.213 96.388 97.561 98.733 99.904 101.074 102.243 103.410 104.576 105.742 106.906 108.069 109.232 110.393 111.553 112.712 113.871 115.028 116.184 117.340 118.495 119.648 120.801 121.954 123.105 124.255 125.405 126.554 127.702 128.849 129.996 131.142 132.287 133.431 134.575 135.718 136.860 138.002 139.143 140.283 141.423 142.562 143.700 144.838 145.975 147.111 83.513 84.733 85.950 87.166 88.379 89.591 90.802 92.010 93.217 94.422 95.626 96.828 98.028 99.228 100.425 101.621 102.816 104.010 105.202 106.393 107.583 108.771 109.958 111.144 112.329 113.512 114.695 115.876 117.057 118.236 119.414 120.591 121.767 122.942 124.116 125.289 126.462 127.633 128.803 129.973 131.141 132.309 133.476 134.642 135.807 136.971 138.134 139.297 140.459 141.620 142.780 143.940 145.099 146.257 147.414 148.571 149.727 150.882 152.037 86.994 88.236 89.477 90.715 91.952 93.186 94.419 95.649 96.878 98.105 99.330 100.554 101.776 102.996 104.215 105.432 106.648 107.862 109.074 110.286 111.495 112.704 113.911 115.117 116.321 117.524 118.726 119.927 121.126 122.325 123.522 124.718 125.913 127.106 128.299 129.491 130.681 131.871 133.059 134.247 135.433 136.619 137.803 138.987 140.169 141.351 142.532 143.712 144.891 146.070 147.247 148.424 149.599 150.774 151.948 153.122 154.294 155.466 156.637 91.335 92.605 93.874 95.140 96.404 97.665 98.925 100.182 101.437 102.691 103.942 105.192 106.440 107.685 108.929 110.172 111.412 112.651 113.889 115.125 116.359 117.591 118.823 120.052 121.280 122.507 123.733 124.957 126.179 127.401 128.621 129.840 131.057 132.273 133.489 134.702 135.915 137.127 138.337 139.546 140.755 141.962 143.168 144.373 145.577 146.780 147.982 149.183 150.383 151.582 152.780 153.977 155.173 156.369 157.563 158.757 159.950 161.141 162.332 94.461 95.751 97.039 98.324 99.607 100.888 102.166 103.442 104.716 105.988 107.258 108.526 109.791 111.055 112.317 113.577 114.835 116.092 117.346 118.599 119.850 121.100 122.348 123.594 124.839 126.083 127.324 128.565 129.804 131.041 132.277 133.512 134.745 135.978 137.208 138.438 139.666 140.893 142.119 143.344 144.567 145.789 147.010 148.230 149.449 150.667 151.884 153.099 154.314 155.528 156.740 157.952 159.162 160.372 161.581 162.788 163.995 165.201 166.406 Page 21 of 22 Analyzing Data from an Experiment 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 160 170 180 190 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 79.692 80.522 81.353 82.185 83.018 83.852 84.686 85.520 86.356 87.192 88.029 88.866 89.704 90.543 91.382 92.222 93.063 93.904 94.746 95.588 96.431 97.275 98.119 98.964 99.809 100.655 101.501 102.348 103.196 104.044 104.892 105.741 106.591 107.441 108.291 109.142 117.679 126.261 134.884 143.545 152.241 196.161 240.663 285.608 330.903 376.483 422.303 468.328 514.529 560.885 607.380 653.997 700.725 747.554 794.475 841.480 888.564 87.213 88.084 88.955 89.827 90.700 91.573 92.446 93.320 94.195 95.070 95.946 96.822 97.698 98.576 99.453 100.331 101.210 102.089 102.968 103.848 104.729 105.609 106.491 107.372 108.254 109.137 110.020 110.903 111.787 112.671 113.556 114.441 115.326 116.212 117.098 117.985 126.870 135.790 144.741 153.721 162.728 208.098 253.912 300.064 346.482 393.118 439.936 486.910 534.019 581.245 628.577 676.003 723.513 771.099 818.756 866.477 914.257 127.531 128.587 129.642 130.697 131.752 132.806 133.861 134.915 135.969 137.022 138.076 139.129 140.182 141.235 142.288 143.340 144.392 145.444 146.496 147.548 148.599 149.651 150.702 151.753 152.803 153.854 154.904 155.954 157.004 158.054 159.104 160.153 161.202 162.251 163.300 164.349 174.828 185.293 195.743 206.182 216.609 268.599 320.397 372.051 423.590 475.035 526.401 577.701 628.943 680.134 731.280 782.386 833.456 884.492 935.499 986.478 1037.431 134.813 135.898 136.982 138.066 139.149 140.233 141.315 142.398 143.480 144.562 145.643 146.724 147.805 148.885 149.965 151.045 152.125 153.204 154.283 155.361 156.440 157.518 158.595 159.673 160.750 161.827 162.904 163.980 165.056 166.132 167.207 168.283 169.358 170.432 171.507 172.581 183.311 194.017 204.704 215.371 226.021 279.050 331.789 384.306 436.649 488.849 540.930 592.909 644.800 696.614 748.359 800.043 851.671 903.249 954.782 1006.272 1057.724 141.030 142.138 143.246 144.354 145.461 146.567 147.674 148.779 149.885 150.989 152.094 153.198 154.302 155.405 156.508 157.610 158.712 159.814 160.915 162.016 163.116 164.216 165.316 166.415 167.514 168.613 169.711 170.809 171.907 173.004 174.101 175.198 176.294 177.390 178.485 179.581 190.516 201.423 212.304 223.160 233.994 287.882 341.395 394.626 447.632 500.456 553.127 605.667 658.094 710.421 762.661 814.822 866.911 918.937 970.904 1022.816 1074.679 146.571 147.700 148.829 149.957 151.084 152.211 153.338 154.464 155.589 156.714 157.839 158.962 160.086 161.209 162.331 163.453 164.575 165.696 166.816 167.936 169.056 170.175 171.294 172.412 173.530 174.648 175.765 176.882 177.998 179.114 180.229 181.344 182.459 183.573 184.687 185.800 196.915 207.995 219.044 230.064 241.058 295.689 349.874 403.723 457.305 510.670 563.852 616.878 669.769 722.542 775.211 827.785 880.275 932.689 985.032 1037.311 1089.531 148.247 149.383 150.517 151.652 152.785 153.918 155.051 156.183 157.314 158.445 159.575 160.705 161.834 162.963 164.091 165.219 166.346 167.473 168.600 169.725 170.851 171.976 173.100 174.224 175.348 176.471 177.594 178.716 179.838 180.959 182.080 183.200 184.321 185.440 186.560 187.678 198.846 209.978 221.077 232.146 243.187 298.039 352.425 406.457 460.211 513.736 567.070 620.241 673.270 726.176 778.972 831.670 884.279 936.808 989.263 1041.651 1093.977 153.191 154.344 155.496 156.648 157.800 158.950 160.100 161.250 162.398 163.546 164.694 165.841 166.987 168.133 169.278 170.423 171.567 172.711 173.854 174.996 176.138 177.280 178.421 179.561 180.701 181.840 182.979 184.118 185.256 186.393 187.530 188.666 189.802 190.938 192.073 193.208 204.530 215.812 227.056 238.266 249.445 304.940 359.906 414.474 468.724 522.717 576.493 630.084 683.516 736.807 789.974 843.029 895.984 948.848 1001.630 1054.334 1106.969 157.808 158.977 160.146 161.314 162.481 163.648 164.814 165.980 167.144 168.308 169.471 170.634 171.796 172.957 174.118 175.278 176.438 177.597 178.755 179.913 181.070 182.226 183.382 184.538 185.693 186.847 188.001 189.154 190.306 191.458 192.610 193.761 194.912 196.062 197.211 198.360 209.824 221.242 232.620 243.959 255.264 311.346 366.844 421.900 476.606 531.026 585.207 639.183 692.982 746.625 800.131 853.514 906.786 959.957 1013.036 1066.031 1118.948 163.523 164.712 165.900 167.088 168.275 169.461 170.647 171.831 173.015 174.198 175.380 176.562 177.743 178.923 180.103 181.282 182.460 183.637 184.814 185.990 187.165 188.340 189.514 190.688 191.861 193.033 194.205 195.376 196.546 197.716 198.885 200.054 201.222 202.390 203.557 204.723 216.358 227.942 239.480 250.977 262.434 319.227 375.369 431.017 486.274 541.212 595.882 650.324 704.568 758.639 812.556 866.336 919.991 973.534 1026.974 1080.320 1133.579 167.610 168.813 170.016 171.217 172.418 173.617 174.816 176.014 177.212 178.408 179.604 180.799 181.993 183.186 184.379 185.571 186.762 187.953 189.142 190.331 191.520 192.707 193.894 195.080 196.266 197.451 198.635 199.819 201.002 202.184 203.366 204.547 205.727 206.907 208.086 209.265 221.019 232.719 244.370 255.976 267.541 324.832 381.425 437.488 493.132 548.432 603.446 658.215 712.771 767.141 821.347 875.404 929.329 983.133 1036.826 1090.418 1143.917 *Taken from http://www.medcalc.org/manual/chi-square-table.php. Page 22 of 22