Refereed Publications and Book Chapters:
1. Jordan, F.; Pullman, B. "Molecular Orbital Calculations of the Preferred Conformation of Nucleosides"
Theoret. Chim.Acta 1968 9, 242-252.
2. Jordan, F.; Pullman, B. "Extended Huckel Calculations on the Conformations and Structure of ThyminePhotodimers" Theoret. Chim. Acta 1968, 10, 423-424 .
3. F. Jordan, "Theoretical Calculation of the Structure and Electronic Properties of Dihydrothymine and
Dihydrouracils" Theoret. Chim. Acta 1968 11, 390-400.
4. Diner, S.; Malrieu, J. P.; Claverie, J. P.; Jordan, F. "Fully Localized Bond Orbitals and the Correlation
Problem" Chem.Phys. Letters 1968, 2, 319-323.
5. Diner, S.; Malrieu, J. P. ; Jordan, F.; Gilbert, M."Localized Bond Orbitals and the Correlation Problem
III. Energy to Third Order in the Zero Differential Overlap Approximation. Application to Sigma Electron
Systems" Theoret. Chim. Acta. 1969 15, 100-110.
6. Jordan, F.; Gilbert, M.; Malrieu, J. P.; Pincelli, U."Localized Bond Orbitals and the Correlation Problem, IV.
Stability of Perturbation Energies with Respect to Bond Hybridization and Polarity" Theoret. Chim. Acta. 1969
15, 211-224.
7. Diner, S. ; Malrieu, J. P.; Jordan, F.; Claverie, P."Use of the Perturbation Method for the Study of the
Configuration Interaction Effect. Localized Self-Consistent Field Orbitals and Second Order Energy
Correction" Theoret. Chim. Acta 1970 18, 86-100.
8. Jordan, F.; McFarquhar, B. Y. "Evidence for a Guanosine-Ca2+ Complex. A Specific Nucleoside-Metal
Interaction" J. Amer. Chem. Soc. 1972, 94, 6657-6658 .
9. Jordan, F.; Sostman, H. D. "A Comparative Molecular Orbital Study of Protonated Adenine Tautomers and
their Intermolecular Interactions" J. Amer. Chem. Soc. 1972 94, 7898-7902.
10. Guthrie, J. P.; Jordan, F. "Amine Catalyzed Decarboxylation of Acetoacetic Acid: The Rate Constant for
Decarboxylation of a -iminoacid" J. Amer. Chem. Soc. 1972 94, 9136-9141.
11. Guthrie, J. P. ; Jordan, F. "Enamine Formation and Hydrolysis: Ethyl -Cyanomethylaminocrotonate"
J. Amer. Chem. Soc. 1972 94, 9132-9136 .
12. Jordan, F. "An Extended Huckel Molecular Orbital Approach to the Study of the Electronic Structures and
Barriers to Syn-Anti Interconversion in Syn Purine Nucleosides" Biopolymers 1973, 12, 243-255.
13. Jordan, F. "The Electronic Structure of and Conformational Energy Barrier to Rotation Around the C-N Glycosidic Linkage in Adenosine-3', 5'-Cyclicmonophosphate (Cyclic AMP)" J. Theoret. Biol. 1973 41, 23-40.
14. Jordan, F. "Lennard-Jones Potential Calculations of the Barrier to Rotation Around the Glycosidic Linkage in
Selected Purine Nucleosides and Nucleotides. A Direct Comparison of the Results of 6-12 Potential Calculations with Results of Semiempirical Molecular Orbital Studies" J. Theoret. Biol. 1973 41, 375-395.
15. Jordan, F.; McFarquhar, B. Y. "Unequivocal Potentiometric Assignment of the Site of Adenosine
Aqueous Base" J. Chem. Soc. Chem. Commun. 1973, 485-6.
Deprotonation in
16. Jordan, F.; Sostman, H. D. "Effect of Protonation on DNA Base Interactions", J.Am.Chem.Soc. 1973 95, 6544-54.
17. Hemmes, P.; Costanzo, J.; Jordan, F. "Ultrasonic Relaxation Evidence for Two Kinds of Ion Pairs in NMethylpyridinium Iodide Solutions" J. Chem. Soc. Chem. Commun. 1973 696-697.
18. Jordan, F. "Acidity Scales in Water-Acetonitrile Mixtures" J. Phys. Chem. 1973 2681-2683.
19. Jordan, F. "Effect of Protonation on the Electronic Structure of Guanosine and Guanosine-5-monophosphate,
and on the Glycosidic Rotations" Biopolymers 1974 13, 289.
20. Jordan, F. "Determination of C-protonation and hydrolysis rates in enamines" J. Am. Chem. Soc. 1974, 96, 825.
21. Jordan, F. "Semiempirical Calculations on the Electronic Structure and Preferred Conformations of Thiamin
(Vitamin B1) and Thiamin Pyrophosphate" J. Am. Chem. Soc. 1974 96, 3623.
22. Jordan, F. "The Purine C-8 Substituent as Probe of Electronic Structure. A Computational Study"
J. Am. Chem. Soc. 1974 96, 5911.
23. Hemmes, P., Oppenheimer, L. ; Jordan, F. "Ultrasonic Evaluation of the Thermodynamics of Syn-Anti
Glycosidic Isomerization in Adenosine" J. Am. Chem. Soc. 1974 96, 6023.
24. Jordan, F. "Non-Empirical Study of the Ion Pair Type Charge Transfer Complex, Pyridinium Hydrochloride"
J. Am. Chem. Soc. 1975 97, 3330.
25 Hemmes, P.;Oppenheimer, L. ; Jordan, F. "Determination of the Thermodynamics of Isomerization
Processes", Proceedings of NATO Summer School on Fast Reaction Kinetics. D. Reidel Publishers (1975).
26. Jordan, F. "Theoretical Studies on Thiamin Action, Conformation of 2-1-Hydroxethylthiamine"
J. Am. Chem. Soc. 1976 , 98, 808.
27. Jordan, F. "Non-empirical Studies on C2NH6+ Ions" J. Phys. Chem. 1976 80, 76.
28. Hall, S S. ; Sha, C-K ; Jordan, F. "Synthesis and 13C Chemical Shifts in Cyclopropyl-Aromatic
Hydrocarbons" J. Org. Chem. 1976 , 41, 1494.
29. Hall, S. S.; Doweyko, A. M. ; Jordan, F. "Glyoxalase I. Evidence for Proton Transfer in the Enzyme
Mechanism" J. Am. Chem. Soc. 1976 98, 7460.
30. Hemmes, P. ; Oppenheimer, L.; Jordan, F. "Ultrasonic Relaxation Evidence for a Two-State Glycosyl
Conformational Equilibrium in Adenosine-3',5'-cyclic-monophosphate" J.C..S. Chem. Commun. 1976 929.
31. Farzami, B. ; Mariam, Y. H.; Jordan, F. "Solvent Effects on Thiamin-Enzyme Model Interactions I. Interaction
with Tryptophan" Biochemistry 1977 16, 1105.
32. Jordan, F.; Mariam, Y. H. "1H and 13C NMR on the Interaction of Mn(II) and Ni(II) with Thiamin and
Thiamin Pyrophosphate" Microchem. Journal 1977 22, 182.
33. Jordan,F.; Niv, H. "Glycosyl Conformational and Inductive Effects on Acid Catalyzed Purine Nucleoside
Hydrolysis" Nucl. Acids Res. 1977 4, 697.
34. Jordan,F.; Farzami, B. "Solvent Effects on Thiamin-Enzyme Model Interactions II.; 2,3,4-Trimethylthiazolium
Iodide, A Model for Interaction with a Negative Charge" J. Chem. Soc. Perkin Trans II. 1977 1482.
35. Jordan, F.; Niv, H. "C8 Aminopurine Nucleosides. A well defined steric determinant of glycosyl
conformational preferences" Biochim. Biophys. Acta 1977 476, 265.
36. Jordan, F.; Bassett,E.; Redwood, W. R. "PMR on Wheat Germ Agglutinin-Amino Sugar Interaction. Evidence
for Involvement of a Tryptophan Residue in the Binding" Biochim. Biophys. Res. Commun. 1977 75, 1015.
37. Renugopalakrishnan, V. ; Jordan, F. " Ab initio calculations on peptides" Vth American Peptide Symposium,
M. Goodman,Editor, 1977.
38. Jordan, F.; Kuo, D. J. ; Monse, E. U.. "C-13 Kinetic Isotope Effects on Pyruvate Decarboxylation Catalyzed
by Yeast Pyruvate Decarboxylase and Models" J. Am. Chem. Soc. 1978, 100, 2872.
39. Jordan, F.; Mariam, Y. H. "N-1'-Methyl Thiamin, A Model for the Role of the Pyrimidine Ring in Thiamin
Pyrophosphate Requiring Enzymatic Reactions" J. Am. Chem. Soc. 1978 100, 2534-2541.
40. Hemmes, P.; Costanzo, J. ; Jordan, F. "Thermodynamics and Kinetics of Association in N-Methylpyridinium
Iodide" J. Phys. Chem. 1978 82, 387.
41. Jordan, F. ; Kuo, D. J.; Monse, E. U. "A pH-Rate Method for Determining the Activity-pH Profile of Enzymes;
Applications to Yeast Pyruvate Decarboxylase - Evidence for Several Ionizable Groups" Anal. Biochem.
1978 86, 298-302.
42. Jordan, F.; Kuo, D. J.; Monse, E. U. "C-13 Kinetic Isotope Effects on Pyruvate Decarboxylation. II Solvent
Effects in Model Systems" J. Org. Chem. 1978 43, 2828.
43. Lalancette, R. A.;Furey, W. ;Hemmes, P. ;Costanzo, J.;Jordan, F. "Crystal and Molecular Structure of NMethylpyridinium Iodide" Acta. Cryst. B. 1978 34, 2950.
44. Hall, S. S.; Doweyko,A. M.; Jordan, F. "Glyoxalase I Studies IV. A General Base Catalyzed Enediol Model
Mechanism" J. Am. Chem. Soc. 1978 100, 5934.
45. Jordan, F.; Wu, A. "Stereoelectronic Factors in the Binding of Substrate Analogs and Inhibitors to Purine
Nucleoside Phosphorylase" J. Med. Chem. 1978 21, 877.
46. Jordan, F.; Wu, A. "Inactivation of Purine Nucleoside Phosphorylase by Modification of Arginine Residues"
Arch. Biochem. Biophys. 1978 190, 699.
47. Jordan, F.; Patrick, J.; Salamone, S. J. "Purine Nucleoside Phosphorylase Breaks the C-O Bond in Ribose1-Phosphate" J. Biol. Chem. 1979 254, 2384.
48. Jordan, F. et al. "Binding of Cofactors to Yeast Pyruvate Decarboxylase" in Catalysis in Chemistry and
Biochemistry B. Pullman, Editor, D. Reidel Dordrecht, Holland 1979,p 131.
49. P. Hemmes, F. Jordan et al, "Urea destacks 6-Methyl Purine. Evidence from Ultrasonic Relaxation"
J. Phys. Chem. 1980 84, 911.
50. Jordan, F.; Nishikawa, S.; Hemmes, P. "Ethidium Bromide Lowers the Barrier to Syn-Anti Glycosyl
Rotation in 2'-Deoxyadenosine" J. Amer. Chem. Soc. 1980 102, 3913.
51. Hemmes, P.;Oppenheimer, L. ; Jordan, F.; Nishikawa, S. "Thermodynamics of Syn-Anti Glycosyl Isomerization in Cyclic-Adenosine-3',5'-Monophosphate in H2O and Aqueous Urea" J. Phys. Chem. 1981 85, 98.
52. Jordan,F.; Bahr, H.; Patrick, J.; Woo, P. W. K. "NMR Studies on Wheat Germ Agglutinin Monomeric
Amino Sugar Interactions" Arch. Biochem. Biophys. 1981 207, 81.
53. Furey, W.; Jordan, F.; Mariam, Y. H.; Lalancette R. A. "X-ray and Molecular Structure of N1'Methylthiaminium Diiodide" Acta Cryst. 1981 B37, 1147.
54. Jordan, F.; Chen, G.; Nishikawa, S.; Sundoro-Wu B "Potential Roles of the Aminopyrimidine Ring in
Thiamin Catalyzed Reactions" Ann. New York Acad. Sci. 1982 378, 14.
55. Barker, J. N.; Jordan, F.; Hillman, D. E; Barlow . O "Phrenic Thiamin and Neuropathy in Sudden Infant
Deaths" Ann. New York Acad. Sci. 1982, 378, 449.
56. Jordan, F.; Polgar, L. "1H NMR Evidence for the Absence of a Stable Hydrogen Bond Between the Active
Site Aspartate and Histidine Residues of Native Subtilisins and for its Presence in Thiolsubtilisins"
Biochemistry 1981 20, 6366.
57. Jordan, F. ; Salamone, S. J.; Wang, A. L. "Application of the 18O Shift on the 31P NMR to the Elucidation of
Biochemical Phosphate Transfer Mechanisms" ACS Symposium Ser. 1981 171, pp 585-589 Washington, D. C.
58. Jordan, F. ; Polgar, L.; Tous, G. "High Field Proton NMR Studies on the Active Site of Subtilisin and
Thiolsubtilisin" Steric Effects in Biomolecules, Elsevier, Holland, 1982 pp 271.
59. Izzo, G. E.; Jordan, F.; Mendelsohn, R. "Resonance Raman and 500 MHz 1H NMR Studies of Tyrosine
Modification in Hen Egg White Lysozyme" J. Am. Chem. Soc. 1982 104, 3178.
60. Jordan, F. "1H NMR Evidence for High Barriers to Amino Group Rotation in 4-Aminopyrimidines, Including
Thiamin, at Low pH in Water" J. Org. Chem. 1982 47, 2748-2753.
61. Salamone, S. J.; Jordan , F.; Jordan, R. "31P NMR Studies on Purine Nucleoside Phosphorylase Catalyzed
Reactions II" Arch. Biochem. Biophys. 1982 217, 139.
62. Hemmes, P.; Nishikawa, S.; Jordan, F. "Ultrasonic Relaxation Studies on a New Relaxation Found in
Nucleoside Phosphates" J. Phys. Chem. 1982 86, 1434.
63. Jordan, F.; Kuo, D. J.; Salamone, S. J.; Wang, S. J. "Determination of the Scissile Bond in the Hydrolysis of
- D-ribofuranose-1-phosphate by Alkaline Phosphatase, Acid Phosphatase, and Formic Acid and in its
Conversion to D-ribose-5-phosphate by Phosphoglucomutase" Biochim. Biophys. Acta 1982 704, 427.
64. Salamone, S. J.; Jordan, F. "Synthesis of 9-(3,4-Dioxopentyl)-hypoxanthine, the First Arg-Directed Purine
Derivative: An Irreversible Inactivator for Purine Nucleoside Phosphorylase" Biochemistry 1982 21, 6383.
65. Jordan, F. et al. "Mechanism-Based Inhibitors of Glyoxalase I" Drug Metabolism Reviews 1983 14, 723.
66. Jordan, F.; Hemmes, P.; Nishikawa, S.; Mashima, M. "Estimation of the Energy Barrier to Syn-Anti
Interconversion in a Pyrimidine Nucleotide: Ultrasonic Investigation of Syn-Anti Glycosyl Isomerization in
Cytidine Cyclic-2',3'-Monophosphate with Bound Ethidium Bromide" J. Am. Chem. Soc. 1983 105, 2055.
67. Kuo, D. J.; Jordan, F. "Active Site Directed Irreversible Inactivation of Brewer's Yeast Pyruvate Decarboxylase
by the Conjugated Substrate Analog 4-(4-Chlorophenyl)-2-oxo-3-butenoic acid" Biochemistry 1983 22, 3735.
68. F. Jordan, et al. "The Synthesis of C6'-Methylthiamin and C6'-Ethylthiamin" Synthesis 1983 555.
69. F. Jordan and R. Fathi, "Mechanism of Phosphate Transfer on the Purine Salvage Pathway 5"
Phosphorus and Sulfur 1983 18, 341.
70. Kuo, D. J.; Jordan, F. "Direct Spectroscopic Observation of a Brewer's Yeast Pyruvate Decarboxylase-Bound
Enamine Intermediate Produced from a Suicide Substrate. Evidence for Non-Concerted Decarboxylation"
J. Biol. Chem. 1983 258, 134l7.
71. Chen, G.; Jordan,F. "Brewer's Yeast Pyruvate Decarboxylase Produces Acetoin from Acetaldehyde etc."
Biochemistry 1984 23, 3576.
72. Jordan, F.; Polgar, L.; Tous, G. "Proton Magnetic Resonance Studies of the States of Ionization of Histidines
in Native and Modified Subtilisins" Biochemistry 1985 24, 7711 .
73. Jordan, F.; Kudzin, Z. H.; Witczak , Z.; Hoops, P. "Nuclear Magnetic Resonance Determination of the Site of
Acylation of the Tautomeric Nucleophile 4-Thioxopyridine" J. Org. Chem. 1986, 51, 571.
74. Kuo, D. J.; Dikdan, G.; Jordan, F. "Resolution of Brewer's Yeast Pyruvate Decarboxylase into Two Isozymes"
J. Biol. Chem. 1986, 261, 3316.
75. Jordan, F.; Kudzin, Z. H.; Kuo, D. J. "Enzyme Generated Model for the Reductive Acetylation of Lipoyl-E2
by E1 in the Pyruvate Dehydrogenase Multienzyme Complex" Ann. N.Y.Acad. Sci. 1986 471, 308
76. Jordan, F.; Adams, J.; Farzami, B.; Kudzin, Z.H. "Conjugated 2-Keto Acids as Mechanism- Based Inactivators
of Brewer's Yeast Pyruvate Decarboxylase: Electronic Effects of Substituents and Detection of a Long-lived
Intermediate" J. Enz. Inhibition 1986 1, 139.
77. Fathi, R.; Jordan, F. " -D-Ribofuranosyl-1,2-cyclicmonophosphate. Isolation, NMR Spectroscopic Properties
and Rates and Mechanism of Acid and Alkaline Hydrolysis" J. Org. Chem. 1986 51, 4143.
78. Jordan, F.; Kudzin, Z.H.; Rios, C.B." Generation and Spectroscopic Characterization of Thiamin-bound
Intermediates in Solution and on Pyruvate Decarboxylase" Studies in Org. Chem. 1987 31, 461.
79. Greenfield, N.; McKenzie, M.; Lenard,J.; Jordan, F.; Adebodun, F. "31P NMR Studies of Insulin Control of
Glucose Metabolism in N. Crassa slime mutant" Ann. N.Y. Acad. Sci. 1987 508, 471.
80. Jordan, F.; Kudzin, Z.H.; Rios, C.B. " Generation and Physical Properties of Enamines Related to the Key
Intermediate in Thiamin Diphosphate Dependent Enzymatic Pathways " J.Am.Chem.Soc. 1987 109, 4415.
81. Jordan, F. ; Akinyosoye, O.; Dikdan, G.; Kudzin , Z.H.; Kuo, D.J."Novel Findings on Brewers' Yeast
Pyruvate Decarboxylase" in Thiamin Pyrophosphate Biochemistry, 1988 Vol 1, R.L. Schowen; A.
Schellenberger Editors, CRC Press, Boca Raton, FL.
82. Fawell, S.E.; McKenzie, M.A.; Greenfield, N.J.; Adebodun, F.; Jordan, F.;Lenard, J. "Stimulation by mam-
malian insulin of glycogen metabolism in a wall-less strain of N. Crassa" Endocrinology 1988 122, 518.
83. Barbato, G. F..; Jordan, F.; Komisaruk, B.R. "The in vitro proteolytic processing of Vasoactive Intestinal
Polypeptide by rat spinal cord homogenate" Ann. N Y. Acad. Sci. 1988 527, 582.
84. Komisaruk, B. R.; Banas, C.; Heller, S.B.; Whipple, B.H.; Barbato, G.F.; Jordan, F. "Analgesia produced by
Vasoactive Intestinal Polypeptide administered directly to the spinal cord in rats" Ann. N. Y. Acad. Sci. 1988
527, 650.
85. Adebodun, F.; Jordan, F." 11B Nuclear Magnetic Resonance of the Structure of the Transition State Analog
Phenylboronic Acid Bound to Chymotrypsin" J.Am.Chem.Soc. 1988 110, 309.
86. Fathi, R.; Jordan, F. " Certain Novel Ribofuranosyl Phosphates Derived from phospho--D-ribofuranosyl-1pyrophosphate" J. Org. Chem. 1988 53, 1997 .
87. Greenfield, N. J.; McKenzie, M. A.; Adebodun, F.; Jordan, F.; Lenard, J. "Metabolism of D-Glucose in a wallless mutant of N. Crassa Examined by 13C and 31P NMR: Effects of Insulin" Biochemistry 1988, 27, 8526.
88. Nelson, R. A..; Pope, J. A.; Pandey, R. C.; McDaniel, L.E. ; Schaffner, C.P.; Beveridge, R. L.; Hoops, P.H.;
Jordan F." Studies on the Biosynthesis of the Antibiotic Crisamicin A and Carbon-13 Magnetic Resonance
Assignments" J. of Antibiotics 1988 41, 1659.
89. Adebodun, F.; Jordan, F. " Multinuclear Magnetic Resonance Studies of Transition State Analogs On Serine
Proteases" J. Cell. Biochem. 1989 40, 249-260.
90. Greenfield, N. J..; Wu, X.; Jordan, F." PMR of Adrenodoxin: Features of the Aromatic Region"
Biochim. Biophys. Acta 1989, 995, 246-254.
91. Zhu, X.; Ohta, Y.; Jordan, F.; Inouye, M. "Pro-sequence of subtilisin can guide the refolding of denatured
subtilisin in an intermolecular process" Nature 1989, 339, 483-484.
92. Adebodun, F.;Jordan, F." Multinuclear Magnetic Resonance Studies on the Ca(II) Binding Site
in Trypsin, Chymotrypsin and Subtilisin" Biochemistry 1989, 28, 7524.
93. Annan, N.; Paris, R.; Jordan , F. " p-Halomethylbenzylidene pyruvic acids inactivate yeast pyruvate
decarboxylase by a variety of mechanisms" J.Am.Chem.Soc. 1989, 111, 8895.
94. Masters, D. B.; Jordan, F.; Komisaruk, B. R. "Regional In Vivo Superfusion of Spinal Cord and KCl-induced
Amino Acid Release" Pharm. Biochem. Beh. 1989 34. 107-112.
95 Tous, G.; Cash, P.; Tsao, J.; Stein, S.; Ohta, Y.; Zhu, X.; Inouye, M.; Jordan, F .
" Synthesis of the Pro-peptide of Pre-pro-subtilisin BPN'" Peptide Research 1989, 2, 292-296.
96. Bordwell , F. G.; Satish , A. V..; Rios, C B.; Chung, A. ; Jordan , F.
"Equilibrium Acidities of 2-Alkylthiazolium Salts" J. Am. Chem. Soc. 1990, 112, 792.
97. Tous, G.; Bush, A.; Tous, A.; Jordan, F. "Epoxyalkyltyrosines as Irreversible Inactivators of Serine Proteases:
Synthesis and Mechanisms of Inactivation" J. Med. Chem. 1990, 33, 1620-1634.
98. Greenfield, N.; Cherapak, N.; Adebodun, F.; Jordan, F.; Lenard , J. "Intracellular Na content in N.Crassa Slime
Mutant, and effects of insulin" Biochim. Biophys. Acta 1990, 1025, 15-20
99. Annan, N.; Jordan, F. "Flavo-Pyruvate Decarboxylase, A semi-synthetic enzyme model for pyruvate oxidase
and acetolactate synthetase" J. Am. Chem. Soc. 1990, 112, 3222-3223.
100. Skawinski, W A.; Flisak, J.; Chung, A.C.; Jordan, F.; Mendelsohn, R. "Synthesis of 15N Tetranitromethane, and its applications to the synthesis of [3-15N]-nitrotyrosine J. Labeled Comp. and Radiopharm.,
1990, 28, 1179-1183.
101. Barletta, G.; Chung, A.C.; Rios, C B.; Jordan, F.; Schlegel. J. M." Electrochemical Oxidation of Enamines
Related to Intermediates in Thiamin Diphosphate Catalyzed Reactions: Evidence for one electron oxidation
and a Thiazolium cation radical " J. Am. Chem. Soc. 1990, 112 , 8144-8149.
102. Dyda, F.; Furey, W.; Swaminathan, S.; Sax, M.; Farrenkopf, B.; Jordan, F. " Preliminary Crystallographic
Data for the Thiamin Diphosphate-dependent Enzyme Pyruvate Decarboxylase from Brewers' Yeast"
J. Biol. Chem. 1990, 265, 17413-17415.
103. B. Farzami, F. Jordan "The Ca metal binding site of proteolytic enzymes: a novel method to elucidate the
amino acid residues involved in binding metals ions in proteins" in Metal Ions in Biology and Medicine
Collery, Ph.; Poirier, L.A.; Manfait, M.; Etienne, J.C. , Ed John Libbey Eurotext, Paris, 1990, 126-128.
103a. Farzami, B and Jordan, F. 1990, Trace Elements in Biology 2, p73.
104. F. Jordan, B. Farzami, " The effect of Ca ions in proteolytic enzymes against the action of inhibitors" in
Metal Ions in Biology and Medicine Collery, Ph.; Poirier, L.A.; Manfait, M.; Etienne, J.C. , Ed John
Libbey Eurotext, Paris, 1990, 129-131.;
105. Dyda, F.; Furey, W.; Swaminathan, S.; Sax, M.; Jordan, F.; Farrenkopf, B. "Multiple Crystal Forms of
Brewers' Yeast Pyruvate Decarboxylase: Characterization and Preliminary Crystallographic Analysis"
Biochemistry and Physiology of Thiamin Diphosphate Enzymes" 1991,H. Bisswanger and J. Ullrich, Ed.
VCH Publishers, pp 115-122.
106. Jordan, F. ; Zeng, X-P.; Menon-Rudolph, S. ; Annan, N.; Barletta, G. ; Chung, A.C.; Rios, C B."
Observation and Properties of the 2--Carbanion (Enamine ) Intermediate on Pyruvate Decarboxylase and in
Chemical Models Biochemistry and Physiology of Thiamin Diphosphate Enzymes" 1991, H. Bisswanger
and J. Ullrich, Ed. VCH Publishers, pp 34-50.
107. Ohta, Y.; Hojo, H.; Aimoto, S.; Kobayashi, T.; Zhu, X.; Jordan, F.; Inouye, M. "Pro-peptide as an
Intermolecular Chaperone: Renaturation of Denatured Subtilisin E with a Synthetic Pro-peptide” Mol.
Microbiol. 1991, 1507-1510.
108. Zeng, X.; Chung, A.; Haran, M.; Jordan, F. " Direct Observation of the Kinetic Fate of a ThDP bound
Enamine Intermediate on Brewers' Yeast Pyruvate Decarboxylase" J. Am. Chem. Soc. 1991, 113, 5842.
109. Chen, Y. T. ; Jordan, F. " The Reactivity of Thiazolium Ions related to Thiamin in Basic Media Can be
Rationalized by Ionic Mechanisms" J. Org. Chem. 1991, 56, 5029-5038.
110. Zhong, S.;Jordan, F. ; Kettner, C. ; Polgar, L. "Observation of Tightly Bound 11B NMR Signal on Serine
Proteases; Direct Solution Evidence for Tetrahedral Geometry around the Boron in the Putative Transition
State Analogs" J. Am. Chem. Soc. 1991 113, 9429-9435.
111. Farrenkopf, B.; Jordan, F. “Resolution of Pyruvate Decarboxylase into Multiple Isoforms with Similar
Subunit Structure and Activity Using HPLC” Protein Expr. Purif. 1992, 3, 101-107.
112. Zhu, X.; Ohta, Y. ; Cash, P.; Tous, G.; Stein, S.; Inouye, M.; Jordan, F." Refolding of Denatured
Subtilisins with the aid of a Synthetic Pro-Peptide; Sequence-specific Effects on Refolding by an
Intermolecular Chaperone" Biochemistry (Life Science Advances), 1992, 11, 35-47.
113. Mazzenga, G. ; Berner, B.; Jordan, F. “The transdermal delivery of zwitterionic drugs II: the flux of zwitterion
salts” J. Controlled Release 1992 20, 163-170.
114. A. McNally, F. Jordan “An Immunochemical Assay Model System for the Sensitive Detection of Pyruvate
Dehydrogenase Complex (PDHc) and its Decarboxylating Subunit (E1)” Biochim Biophys Acta
1992, 1160, 179-187.
115. Barletta, G.; Huskey, W. P.; Jordan, F. “Observation of a 2--Enamine from a 2-[-Methoxy--phenylmethyl]- 3,4- dimethylthiazolium Salt in Water: Implications for Catalysis by Thiamin Diphosphate-dependent
-Keto Acid Decarboxylases” J. Am. Chem. Soc. 1992 114, 7607-7608.
116. Menon-Rudolph, S.; Nishikawa, S.; Zeng, X.; Jordan, F. “Rate of Decarboxylation, Monitored via the Key
Enzyme-bound Enamine, of Conjugated a-Keto Acids by Pyruvamide Activated Pyruvate Decarboxylase is
Kinetically Competent with Turnover “ J. Am. Chem. Soc. 1992 114, 10110-10112..
117. Skawinski, W. S.; Adebodun, F.; Cheng, J. T; Jordan, F.; Mendelsohn, R. " 15N NMR on Nitrotyrosyl and
Aminotyrosyl Ribonuclease and Lysozyme-Specific Introduction of 15N via (15NO2)4C Biochim. Biophys.
Acta 1993 1162 , 297-308.
118. Zeng, X.; Farrenkopf, B.; Hohmann, S.; Jordan, F. “Role of Cysteines in the Activation and Inactivation of
Brewers’ Yeast Pyruvate Decarboxylase Investigated with a PDC1-PDC6 Fusion Protein
Biochemistry 1993, 32, 2704-2709.
119. Dyda, F.; Furey, W.; Swaminathan, S. ; Sax, M. ; Farrenkopf , B.; Jordan, F. " Catalytic Centers in the Thiamin
Diphosphate Dependent Enzyme Pyruvate Decarboxylase at 2.4Å Resolution" Biochemistry 1993, 32, 6165-6170.
120. Masters, D.; Jordan, F.; Beyer, C.; Komisaruk, B. R. " Release of Amino Acids into Regional Superfusates of
the Spinal Cord by Mechano-Stimulation of the Reproductive Tract" Brain Res. 1993, 621, 279-290.
121. Muller, Y. A.; Lindquist, Y.; Furey, W.; Schulz, G.; Jordan, F.; Schneider, G. "The Thiamin Diphosphate
Binding Fold" Structure 1993, 1, 95-103.
122. Hu, Z.; Zhu, X.; Jordan, F.; Inouye, M. "A Covalently Trapped Folding Intermediate of Subtilisin E:
Spontaneous Dimerization of a Pro-subtilisin E Ser49Cys Mutant in vivo and its Autoprocessing in vitro"
Biochemistry, 1994, 33, 562-569.
123. Baburina, I.; Gao, Y.; Hu, Z.; Hohmann, S.; Furey, W.; Jordan, F. " Substrate Activation of Brewers' Yeast
Pyruvate Decarboxylase is Abolished by Mutation of Cysteine221 to Serine" Biochemistry 1994, 33, 5630-5635.
124. Chiu, C-F.; Jordan, F. " Novel Synthesis of 2-Oxo-4-phenyl-3-butynoic Acid, a new Inhibitor and Alternate
Substrate of Pyruvate Decarboxylase" J. Org. Chem. 1994 59, 5763-5766.
125. Chen, Y. T. ; Barletta, G. B.; Haghjoo, K.; Cheng, J. T.; Jordan, F. " Reactions of Benzaldehyde with
Thiazolium Salts in Me2SO: Evidence for Initial Formation of 2--(hydroxybenzyl)thiazolium by
Nucleophilic Addition and for Dramatic Solvent Effects on Benzoin Formation"
J. Org. Chem. 1994 59, 7714-7722.
126. Jordan, F. (1994) "Biochemistry of the Enamines" in Enamines Ed. by Z. Rappoport, in the series "Chemistry
Of the Functional Groups", S. Patai, Series ed. Wiley Interscience, Chittenden, UK. pp 1253-1302.
127. Jordan, F.; Hu, Z.; Haghjoo, K. (1995) " Interaction of Subtilisin with its Pro-sequence- Versatility of an Nterminal Extension on a Secreted Protein" in "Pro-Peptide Mediated Folding" Shinde, U. and Inouye, M.
Editors, R. G. Landes, and Co. Texas, pp 113-144.
128. Zhong, S.; Haghjoo, K., Kettner, C., Jordan, F. "Proton Magnetic Resonance Studies of the Active Center
Histidine of Chymotrypsin Complexed to Peptideboronic Acids: Solvent Accessibility to the Nd and Ne Sites
can Differentiate Slow-Binding and Rapidly Reversible Inhibitors" J. Am. Chem. Soc. 1995, 117, 7047-7055.
129. Chiu, C. F. Pan, K., Jordan, F. "Modeling an elementary step of the flavoenzyme pyruvate oxidase: oxidation
of a thiamin diphosphate-bound enamine intermediate by a flavin analog" J. Am. Chem. Soc. 1995, 117,
7027-7028.
130. McNally, A., Motter, K., Jordan, F. A Library of Monoclonal Antibodies to E. coli K-12 Pyruvate
Dehydrogenase Complex: A Biochemical Analysis and Their Ability to Inhibit the Enzyme Complex
J. Biol.Chem. 1995, 270, 19736-19743.
131. McNally, A., Mattsson, L. Jordan, F. "A Library of Monoclonal Antibodies to E. coli K-12 Pyruvate
Dehydrogenase Complex: Competitive Epitope Mapping Studies" J. Biol. Chem. 1995, 270, 19744-19751.
132. Li, Y., Hu, Z., Jordan, F., Inouye, M. “ Functional Analysis of the Propeptide of Subtilisin E as an
Intramolecular Chaperone for Protein Folding” J. Biol. Chem. 1995, 270, 25127-25132.
133. Hu, Z., Haghjoo, K., Jordan, F. “ Further evidence for the structure of the subtilisin pro-peptide and for its
interactions with mature subtilisin” J. Biol. Chem. 1996, 271, 3375-3384.
134. Arjunan, P., Umland, T., Dyda, F., Swaminathan, S., Furey, W., Sax, M., Farrenkopf, B., Gao, Y., Zhang, D.,
Jordan, F. “Crystal structure of the thiamin diphosphate dependent enzyme pyruvate decarboxylase from the
yeast Saccharomyces cerevisiae at 2.3 Å resolution” J. Mol. Biol. 1996 256, 590-600.
135. Furey, W., Arjunan, P., Chen, L., Dyda, F., Umland, T., Swaminathan, S., Sax, M., Jordan, F., Farrenkopf. B.,
Gao., Y., Zhang., D. “ Multiple modes of tetramer assembly and insight into allosteric activation revealed by
X-ray crystal structures of pyruvate decarboxylase” in Biochemistry and Physiology of Thiamin Diphosphate
Enzymes”, Bisswanger, H. and Schellenberger, A. Editors, 1996, A. u. C. Intemann Verlag, Prien, Germany
pp.103-124.
136. Nemeria, N., Brown, A., Yi, J-Z., Jordan, F. “2-Oxo-3-alkynoic acids; potent irreversible inactivators of
pyruvate dehydrogenase multienzyme complex from E. coli “ in Biochemistry and Physiology of Thiamin
Diphosphate Enzymes”, Bisswanger, H. and Schellenberger, A. Editors, 1996 A. u. C. Intemann Verlag, Prien,
Germany pp. 334-340.
137. Jordan, F., Baburina, I., Gao, Y., Kahyaoglu, A., Nemeria, N., Volkov, A., Yi, J-Z., Zhang, D., Machado, R.,
Guest, J. R., Furey, W., Hohmann, S. “New insights to the regulation of thiamin diphosphate dependent
decarboxylases by substrate and ThDP.Mg(II)” in Biochemistry and Physiology of Thiamin Diphosphate
Enzymes”, Bisswanger, H. and Schellenberger, A. Editors, 1996 A. u. C. Intemann Verlag, Prien, Germany
pp. 53-69.
138. Baburina, I., Moore , D. J., Volkov, A., Kahyaoglu, A., Jordan, F. and Mendselsohn, R. “ Three of Four
Cysteines, Including that Responsible for Substrate Activation Are Ionized at pH 6.0 in Yeast Pyruvate
Decaboxylase: Evidence from Fourier Transform Infrared and Isoelectric Focusing Studies” Biochemistry
1996, 35, 10249-10255.
139. Yi, Ji-Zu . Nemeria, N., McNally, A., Jordan, F., Guest, J. R., Machado, R. “Effect of Mutations in the
Thiamin Diphosphate-Mg Fold on the Activation and Inhibition of the Pyruvate Dehydrogenase Complex
from E. coli.” J. Biol.Chem. 1996, 271, 33192-33200.
140. Chiu, Chingfan C. Chung, A., Barletta, G., Jordan, F. “ Intramolecular Models for the Reductive Acyl
Transfer Catalyzed by -Keto Acid Dehydrogenases” J. Am. Chem. Soc. 1996, 118, 11026-11029.
141 Volkov, A., Jordan, F. “Evidence for Intramolecular Processing of Prosubtilisin Sequestered on a Solid
Support” J. Mol. Biol. 1996, 262(5), 595-599.
142. Haghjoo, K., Cash, P. W., R. S. Farid, Jordan, F., Pochapsky, S. S. & Komisaruk, B. R.“Solution Structure
of Vasoactive Intestinal Polypeptide(11-28)-NH2, a Fragment with Analgesic Properties “ Peptide Res.
1996, 19 (6), 327-333.
143. Kahyaoglu, A., Haghjoo, K., Felfoldi, F., Kraicsovits, F., Jordan, F., Polgar, L. “Prolyl-Prolinal, a Transition
State Analog for Prolyl Oligopeptidase forms a Tetrahedral Adduct with a Catalytic Serine, not a Reactive
Cysteine“ Biochem. J. 1997, 322 839-843.
144. Barletta, G. L., Huskey, W. P., Jordan, F. “Ionization kinetics at the C2 position of 2-benzylthiazolium
salts leading to enamines relevant to thiamin catalyzed enzymatic reactions” J. Am. Chem. Soc. 1997, 119,
2356-2362.
145. Brown, A., Nemeria, N., Yi, J, Zhang, D., Jordan., W. B., Machado, R.S., Guest, J. R. & Jordan, F. “2-Oxo-3alkynoic acids, universal mechanism-based inactivators of thiamin diphosphate-dependent decarboxylases.
Synthesis and evidence for potent inactivation of the pyruvate dehydrogenase multienzyme complex.”
Biochemistry 1997, 36, 8071-8081.
146. Kahyaoglu, A., Haghjoo, K., Guo, F. , Jordan, F., Kettner, C., Felfoldi, F., and Polgar, L., “Low-Barrier
Hydrogen Bond is Absent in the Catalytic Triads in the Ground State but is Present in the Transition State
Complex in the Prolyl Oligopeptdase Family of Serine Proteases” J. Biol. Chem. 1997 271, 25547-25554.
147. Nemeria, N., Volkov, A., Brown, A.., Yi, J. Zipper, L., Guest, J. R.. & Jordan, F. “Systematic Study of all Six
Cysteines of the E1 Subunit of the Pyruvate Dehydrogenase Multienzyme Complex from Escherichia coli:
None is Essential for Activity” Biochemistry 1998 37, 911-922.
148. Pan, K.,& Jordan, F. S-Methyllipoate, a kinetically viable model for S-protonated lipoic acid as the oxidizing
agent in reductive acyl transfers catalyzed by the 2-oxoacid dehydrogenase multienzyme complexes.
Biochemistry 1998, 37 1357-1364.
149. Baburina, I., Li, H., Furey, W., Bennion. B., Jordan, F. “ Interdomain Information Transfer During Substrate
Activation of Yeast Pyruvate Decarboxylase: The Interaction between Cysteine 221 and Histidine 92”
Biochemistry 1998 37, 1235-1244.
150. Baburina, I., Dikdan, G., Guo, F., Tous, G., Root, B., Jordan, F. “ Reactivity at the Subsrate Activation Site
of Yeast Pyruvate Decarboxylase: Inhibition by Distortion of Domain Interactions”
Biochemistry 1998 37, 1245-1255.
151. Jordan, F., Nemeria, N., Guo, F., Baburina,, I., Gao, Y., Kahyaoglu, A., Li., H., Wang, J., Yi, J., Guest, J. R.,
Furey, W. “ Regulation of thiamin diphosphate-dependent 2-oxoacid decarboxylases by substrate and
thiamin diphosphate.Mg(II)- evidence for tertiary and quaternary interactions. BBA 1998, 1385 287-306.
152. Furey, W., Arjunan, P., Chen, L., Sax, M., Guo, F. Jordan, F. “Structure-function relationships and flexible
tetramer assembly in pyruvate decarboxylase revealed by analysis of crystal structures” BBA 1998 1385,
253-270.
153. Bao, D., Cheng, J-T., Kettner, C. & Jordan, F. “Assignment of the NH and NH Resonances at the
Active Center Histidine in Chymotrypsin and Subtilisin Complexed to Peptideboronic Acids without Specific
N-15 Labeling” J. Am. Chem. Soc. 1998 120, 3485-3489.
154. Guo, F., Zhang, D., Kahyaoglou, A., Farid, R. S., Jordan, F. “ Is a Hydrophobic Amino Acid Required to
Maintain the Reactive V Conformation at the Active Center of Thiamin Diphosphate-Requiring Enzymes?
Experimental and Computational Studies of Isoleucine 415 of Yeast Pyruvate Decarboxylase”
Biochemistry 1998, 37, 13379-13391.
155. Kuramoto, N., Nishikawa, S., Jordan, F. “Kinetic Study in Aqueous Solutions of Nucleotides by Ultrasonic
Relaxation Methods’ J. Phys. Chem. 1998 102, 9181-9186.
156. Eberhardt, I., Cederberg, H., Konig, S., Li, H., Jordan, F., Hohmann, S. “Autoregulation of yeast pyruvate
decarboxylase gene expression requires the enzyme but not its catalytic activity”Eur.J.Biochem. 1999 262,
191-202.
157. Bao, D., Huskey, W. P., Kettner, C., Jordan, F. ‘Hydrogen Bonding to Active-Site Histidine in Peptidyl Boronic
Acid Inhibitor Complexes of Chymotrypsin and Substilisin: Proton Magnetic Resonance Assignments and H/D
Fractionation Factors’ J. Am. Chem. Soc. 1999, 121, 4684-4689.
158. Nishikawa, S., Kuramoto, N., Huang, H. and Jordan, F. ‘Kinetic and Thermodynamic Study of Syn-Anti
Isomerization in Aqueous Solutions of AMP, ADP, ATP by Ultrasonic Relaxation Methods. J. Phys. Chem. B
1999, 103, 3754-3757.
159. Jordan, F., Li, H., Brown, A. “Remarkable Stabilization of Zwitterionic Intermediates May Account for a
Billion-fold Rate Acceleration by Thiamin Diphosphate-dependent Decarboxylases” Biochemistry 1999 38,
6369-6373.
160. Li, H., Furey, W., Jordan, F. “ Role of Glutamate 91 in Information Transfer during Substrate Activation of
Yeast Pyruvate Decarboxylase” Biochemistry 1999, 38 9992-10003.
161. .Li, H., Jordan, F. “Effects of Substitution of Tryptophan 412 in the Substrate Activation Pathway of Yeast
Pyruvate Decarboxylase” Biochemistry 1999, 38 10004-10012.
162. Jordan, F. “Interplay of organic and biological chemistry in understanding coenzyme mechanisms: example
of thiamin diphosphate-dependent decarboxylations of 2-oxo acids” FEBS Lett. 1999, 457 298-301.
163. Nishikawa, S., Huang, H., Jordan, F. “Structural Effect of Nucleotides on Syn-Anti Glycosyl Isomerization
Kinetics by Ultrasonic Relaxation Methods” J. Phys. Chem. 2000, 104 1391-1394.
164. Sergienko, E. A., Wang, J., Polovnikova, L., Hasson, M. S., McLeish, M.J., Kenyon, G. L. and Jordan F.
“Spectroscopic Detection of Transient Thiamin Diphosphate-Bound Intermediates on Benzoylformate
Decarboxylase” Biochemistry 2000, 39, 13862-13869.
165. Wang, J., Golbik, R.., Seliger, B., Spinka, M., Tittmann, K., Hübner, G., and Jordan, F.
“Consequences of a Modified Putative Substrate-activation Site on Catalysis by Yeast Pyruvate Decarboxylase”.
Biochemistry 2001, 40, 1755-1763.
166. Liu, M., Sergienko, E. A., Guo, F., Wang, J., Tittmann, K., Hübner, G., Furey, W. and Jordan, F.
Catalytic Acid-Base Groups In Yeast Pyruvate Decarboxylase I. Site Directed Mutagenesis And Steady-State
Kinetic Studies On The Enzyme With The D28a, H114F, H115F And E477Q Substitutions.
Biochemistry 2001, 40, 7355-7368.
167. Sergienko, E. A., and Jordan, F. Catalytic Acid-Base Groups In Yeast Pyruvate Decarboxylase II. Insights To The
Specific Roles Of D28 and E477 From The Rates And Stereospecificity Of Formation Of Carboligase Side
Products. Biochemistry 2001, 40, 7369-7381.
168. Sergienko, E. A., and Jordan, F. Catalytic Acid-Base Groups In Yeast Pyruvate Decarboxylase III. A SteadyState Kinetic Model Consistent with The Behavior Of Both Wild-Type And Variant Enzymes At All Relevant
pH Values. Biochemistry 2001, 40, 7382-7403.
169. Nemeria, N., Yan, Y., Zhang, Z., Brown, A.M., Arjunan, P., Furey, W., Guest, J.R. and Jordan, F.. Inhibition of the
E. coli Pyruvate Dehydrogenase Complex-E1 Subunit and its Tyrosine 177 Variants by Thiamin 2-Thiazolone
and Thiamin 2-Thiothiazolone Diphosphates: Evidence for Reversible Tight-binding Inhibition.
J. Biol. Chem. 2001 276, 45969-45978.
170. Wei, W., Min Liu, M. and Jordan, F. Solvent Kinetic Isotope Effects Monitor Changes in Hydrogen Bonding at
the Active Center of Yeast Pyruvate Decarboxylase Concomitant with Substrate Activation. The Substituent at
Position 221 Can Control the State of Activation. Biochemistry 2002 451-461.
171. Kahyaoglu, A. and Jordan, F. Direct Proton Magnetic Resonance Determination of the pKa of the Active Center
Histidine in Thiolsubtilisin. Protein Science 2002 11, 965-973.
172. Sergienko E. A. and Jordan, F. A New Model For Activation Of Yeast Pyruvate Decarboxylase By Substrate \
Consistent With The Alternating Sites Mechanism: Demonstration Of The Existence Of Two Active Forms Of
The Enzyme Biochemistry 2002 41, 3952-3967.
173. Arjunan, P., Nemeria, N., Brunskill, A., Chandrasekhar, K., Sax, M., Yan, Y., Jordan, F., Guest J.R. and Furey, W.
Structure of the Pyruvate Dehydrogenase Multienzyme Complex E1 Component from E. coli at 1.85 Å Resolution.
Biochemistry 2002 41(16); 5213-5221.
174. Sergienko, E.A., and Jordan, F. Yeast Pyruvate Decarboxylase Tetramers can Dissociate into Dimers along
Two Interfaces. Hybrids of Low-activity D28A (or D28N) and E477Q Variants, with Substitution of Adjacent
Active Center Acidic Groups from Different Subunits, Display Restored Activity. Biochemistry 2002 41, 6164-6169.
175. Jordan, F., Zhang, Z., and Sergienko, S. Spectroscopic Evidence for Participation of the 1’,4’-Imino Tautomer
of Thiamin Diphosphate in Catalysis by Yeast Pyruvate Decarboxylase. Bioorganic Chem. 2002, 30, 188-198.
176. Nemeria, N., Arjunan, P., Brunskill, A. Sheibani, F. Wei, W., Yan, Y., Zhang, S. Jordan, F., and Furey, W.,
‘Histidine 407, a Phantom Residue in the E1 Subunit of the Escherichia coli Pyruvate Dehydrogenase Complex,
Activates Reductive Acetylation of Lipoamide on the E2 Subunit. An Explanation for Conservation of Active
Sites between the E1 Subunit and Transketolase. Biochemistry 2002, 41, 15459-15467. Accelerated publication
177. Jordan, F. Current Mechanistic Understanding of Thiamin Diphosphate-dependent Enzymatic Reactions Natural
Product Reports 2003, 20, 184-201.
178. Wei, W., Li, H., Nemeria, N., and Jordan, F. “Expression and Purification of the Dihydrolipoamide
Acetyltransferase and Dihydrolipoamide Dehydrogenase Subunits of the Escherichia coli Pyruvate
Dehydrogenase Multienzyme Complex. A Mass Spectrometric Assay for Reductive Acetylation of
Dihydrolipoamide Acetyltransferase Prot. Exp. Purif. 2003, 28, 140-150.
179. Polovnikova, E. S., McLeish, M. J., Sergienko, E.A., Burgner, J.T., Anderson, N.L., Bera, A.K. Jordan, F.,
Kenyon, G.L., and Hasson, M.S. “Structural and Kinetic Analysis of Catalysis by a Thiamin DiphosphateDependent Enzyme Benzoylformate Decarboxylase” Biochemistry, 2003, 42, 1820-1830.
180. Nishikawa, S., Ohno, T., Huang, H., Yoshizuka, K. and Jordan, F. “Ultrasonic absorption in aqueous solutions of
amino acids at neutral pH” J. Acoust. Soc. Am. 2003, 113, 2884-2888.
181. Tittmann, K., Golbik, R., Uhlemann, K., Khailova, L., Schneider, G., Patel, M., Jordan, F., Chipman, D.M.,
Duggleby, R.G. and Hubner, G., “Snapshots of Thiamin-dependent Enzymes in Solution” Biochemistry 2003
42, 7885-7891. Accelerated publication.
182. Jordan, F., Nemeria, N.S., Zhang, S., Yan, Y., Arjunan, P., Furey, W. ‘Dual Catalytic Apparatus of the Thiamin
Diphosphate Coenzyme: Acid-base via the 1’.4’-Iminopyrimidine Tautomer along with its Electrophilic
Role”. J. Am. Chem. Soc. 2003, 127, 12732-12738.
183. Arjunan, P., Chandrasekhar, K., Sax, M., Brunskill, A., Nemeria, N., Jordan, F., Furey, W. “Structural Features
Determining Enzyme-Inhibitor Affinity: The E1 Component of Pyruvate Dehydrogenase from Escherichia coli
In Complex with the Inhibitor Thiamin 2-Thiazolone Diphosphate” Biochemistry 2004 43, 2405-2411.
184. Jordan, F., Liu, M., Sergienko, E., Zhang, Z., Brunskill, A., Arjunan, P., Furey, W. “Yeast Pyruvate
Decarboxylase: New Features of the Structure and Mechanism” in Thiamine: Catalytic Mechanisms and Role
in Normal and Disease States, Jordan, F. and Patel, M. Editors, Marcel Dekker, New York, 2004 pp 173-215.
185. Furey, W., Arjunan, P., Brunskill, A., Chandrasekhar, K., Nemeria, N., Wei, W., Yan, Y., Zhang, Z., Jordan, F.
“Structure and Intersubunit Information Transfer in the Escherichia coli Pyruvate Dehydrogenase
Multienzyme Complex” in Thiamine: Catalytic Mechanisms and Role in Normal and Disease States,
Jordan, F. and Patel, M. Editors, Marcel Dekker, New York, 2004, pp 407-432.
186. Wei, W., Liu, M., Chen, L., Huskey, W. P., Jordan, F., “Solvent and Carbon Kinetic Isotope Effects on ActiveSite and Regulatory-Site Variants of Yeast Pyruvate Decarboxylase” in Thiamine: Catalytic Mechanisms and
Role in Normal and Disease States, Jordan, F. and Patel, M. Editors, Marcel Dekker, New York, 2004, pp 217-31.
187. Tittmann, K., Golbik, R., Uhlemann, K., Hubner, G., Khailova, L., Patel, M., Jordan, F., Chipman, D., Duggleby,
R.G. “How Thiamine Works in Enzymes: Time-Resolved NMR Snapshots of TDP-Dependent Enzymes in
Action” in Thiamine: Catalytic Mechanisms and Role in Normal and Disease States, Jordan, F. and Patel, M.
Editors, Marcel Dekker, New York, 2004, pp 57-76.
188. Nemeria, N., Baykal, A., Joseph, E., Zhang, S., Yan, Y., Furey, W., Jordan, F. “Tetrahedral Intermediates in
Thiamin Diphosphate-Dependent Decarboxylations Exist as a 1’,4’-Imino Tautomeric Form of the Coenzyme,
unlike the Michaelis Complex or the Free Coenzyme’ Biochemistry 2004, 43, 6565-6575.
189. Park, Y-H., Wei, W., Zhou, L., Nemeria, N., and Jordan, F. “The Amino-terminal 1-45 Residues of the
Escherichia coli Pyruvate Dehydrogenase Complex-E1 Subunit Interact with the E2 Subunit and are Required
for Activity of the Complex but not for Reductive Acetylation of the E2 Subunit.” Biochemistry 2004, 43,
14037- 14046.
190. Jordan, F. “How Active Sites Communicate in Thiamine Enzymes” Science, Washington DC 2004, 306, 818820. Perspective on paper by Frank et al. In Science, Washington DC 2004, 306, 872-876.
191. Zhang, S., Liu, M., Yan, Y., Zhang, Z. and Jordan, F. "C2-Lactylthiamin Diphosphate is an Intermediate on
the Pathway of Thiamin Diphosphate-dependent Pyruvate Decarboxylation: Evidence on Enzymes and
Models" J.Biol.Chem. 2004, 279, 54312-54318.
192. Jordan, F., and Nemeria, N. ‘Experimental Observation of Thiamin Diphosphate-bound Intermediates on
Enzymes and the Mechanistic Information Derived from these Observations. Bioorg. Chem. 2005, 33, 190-215.
193. Zhang, S., Zhou, L., Nemeria, N., Yan, Y., Zhang, Z., Zou, Y., and Jordan, F. ‘Evidence for Dramatic Rate
Acceleration of an C-H Bond Ionization Rate in Thiamin Diphosphate Enzymes by the Protein Environment’
Biochemistry 2005, 44, 2237-2243; Accelerated Publication.
194. Nemeria, N., Tittmann, K., Joseph, E., Zhou, L., Vazquez-Coll, M.B., Arjunan, P., Hubner, G., Furey, W. and
Jordan, F. ‘Glutamate 636 of the E. coli Pyruvate Dehydrogenase-E1 Participates in Active Center
Communication and Behaves as an Engineered Acetolactate Synthase with Unusual Stereoselectivity’
J.Biol.Chem. 2005, 280, 21473-21482.
195. Jordan, F., Nemeria, N., Sergienko. E. ‘Multiple Modes of Active Center Communication in Thiamin
Diphosphate-dependent Enzymes’. Acc. Chem. Res. 2005, 38, 755-763.
196. Arjunan, P., Sax, A., Brunskill, A., Chandrasekhar, N., Nemeria, N., Zhang, S., Jordan, F., Furey, W. ‘A
Thiamin-bound Pre-Decarboxylation Intermediate Analogue in the Pyruvate Dehydrogenase E1 Subunit
Induces Large Scale Disorder-to-Order Transformations in the Enzyme and Reveals Novel Structural Features
In the Covalently Bound Adduct’ J. Biol. Chem. 2006 281, 15296-15303.
197. Baykal, A., Kakalis, L., Jordan, F. "Electronic and Nuclear Magnetic Resonance Spectroscopic Features of the
1'4'-Iminopyrimidine Tautomeric form of Thiamin Diphosphate- a Novel Intermediate on Enzymes Requiring
this Coenzyme." Biochemistry 2006 45, 7522-7528.
198. Baykal, A., Chakraborty, S., Dodoo, A. and Jordan, F. Synthesis with good enantiomeric excess of both
enantiomers of -ketols and acetolactates by two thiamin diphosphate dependent decarboxylases, Bioorg. Chem.
2006 34, 380-393.
199. Nemeria, N., Korotchkina, L., Chakraborty, S. Patel, M.S., Jordan, F. Acetylphosphinate is the most potent
mechanism-based substrate-like inhibitor of both the human and E. coli pyruvate dehydrogenase component of
the pyruvate dehydrogenase complex. Bioorg. Chem. 2006 34, 362-379.
200. Joseph, E., Wei, W. Tittmann, K., Jordan, F. Function of a conserved loop of the -domain, not involved in
thiamin diphosphate binding, in catalysis and substrate activation in yeast pyruvate decarboxylase. Biochemistry
2006 45, 13517-13527.
201. Chandrasekar, K., Arjunan, P., Sax, M., Nemeria, N., Jordan, F., Furey, W. Active-site changes in the pyruvate
dehydrogenase multienzyme complex E1 apoenzyme component from Escherichia coli observed at 2.32Å
resolution. Acta Cryst. 2006 D62, 1382-1386.
202. Nemeria, N., Chakraborty, S., Baykal, A., Korotchkina, L., Patel, M.S., Jordan, F. The 1’,4’-Iminopyrimidine Tautomer
of Thiamin Diphosphate is Poised for Catalysis in Asymmetric Active Centers on Enzymes. Proc. Natl. Acod. Sci. USA 2007,
104, 78-82.
203. Schweitzer-Stenner, R., Measey, T., Kakalis, L., Jordan, F., Pizzanelli, S., Forte, C., and Griebenow, K.
Conformations of Alanine Based Peptides in Water Probed by FTIR, Raman, Vibrational Circular Dichroism,
Electronic Circular Dichroism, and NMR Spectroscopy. Biochemistry 2007, 46, 1587-1596.
204. Jordan, F. Adenosine triphosphate and thiamine cross paths. Nature Chem. Biol. 2007 3, 202-203
News and views- invited commentary on paper in the same issue of the journal.
205. Kale, S., Arjunan, P., Furey, W., Jordan, F. A Dynamic Loop at the Active Center of the Escherichia coli Pyruvate
Dehydrogenase Complex E1 Component Modulates Substrate Utilization and Chemical Communication with the
E2 Component J.Biol.Chem. 2007, 282, 28106-28116.
206. Nemeria, N., Korotchkina, L., McLeish, M.J., Kenyon, G.L., Patel, M.S., and Jordan, F. Elucidation of the
chemistry of enzyme-bound thiamin diphosphate prior to substrate binding: defining internal equilibria among
tautomeric and ionization states. Biochemistry 2007, 46, 10739-10744.
207. Kale, S., Ulas, G., Song, J., Brudvig, G.W., Furey, W. and Jordan, F. (2008) Efficient Coupling of Catalysis and
Dynamics in the E1 Component of Escherichia coli Pyruvate Dehydrogenase Multienzyme Complex, Proc. Natl.
Acad. Sci. USA 105, 1158-1163.
208. Chakraborty, S., Nemeria, N., Yep, A., McLeish, M.J., Kenyon, G.L., and Jordan, F. (2008) The Mechanism of
Benzaldehyde Lyase Studied via Thiamin Diphosphate-Bound Intermediates and Kinetic Isotope Effects.
Biochemistry 47 3800-3809.
209. Brandt, G., Nemeria, N., Chakraborty, S., McLeish, M.J., Kenyon, G.L., Petsko, G., Jordan, F., and Ringe, D.
(2008) Probing the active center of benzaldehyde lyase with substitutions and the pseudo-substrate analogue
benzoylphosphonic acid methyl ester Biochemistry 47, 7734-7743.
210. Chakraborty, S., Nemeria, N., Balakrishnan, A., Brandt, G., Kneen, M., Yep, A., McLeish, M., Kenyon, G., Petsko,
G., Ringe, D., Jordan, F. (2009) Detection and time-course of formation of major thiamin diphosphate-bound covalent
intermediates derived from a chromophoric substrate analogue on benzoylformate decarboxylase, Biochemistry 48,
981-94.
211. Jordan, F., Arjunan, P., Kale, S., Nemeria, N.S., Furey, W. (2009) Multiple roles of mobile active center loops in the
E1 component of the Escherichia coli pyruvate dehydrogenase complex - Linkage of protein dynamics to catalysis.
J.Mol.Cat. B Enzymology 61 14-22.
212. Nemeria, N.S., Chakraborty, S., Balakrishnan, A., Jordan, F. (2009) Defining states of ionization and
tautomerization of the coenzyme at individual steps on thiamin diphosphate dependent enzymatic pathways.
FEBS Journal 276, 2432–2446.
213. Brandt, G; Kneen, M; Chakraborty, S; Baykal, A; Nemeria, N; Yep, A; Ruby, D; Petsko, GA; Kenyon, GL;
McLeish, M; Jordan, F; Ringe, D (2009) Snapshot of a reaction intermediate: analysis of benzoylformate
decarboxylase in complex with a benzoylphosphonate inhibitor. Biochemistry 48, 3247–3257.
214. Kovach, I; Kelley, P; Eddy, C; Jordan, F; Baykal, A. (2009) Proton Bridging in the Interactions of Thrombin with
Small Inhibitors. Biochemistry 48 7296-7304.
215. Kale, S., Jordan, F. (2009) Conformational Ensemble Modulates Cooperativity in the Rate Determining Catalytic
Step in the E1 Component of Escherichia coli Pyruvate Dehydrogenase Multienzyme Complex. J.Biol.Chem. 284,
33122-33129.
216. Song, J., Park, Y-H, Nemeria, N.S, Kale, S., Kakalis, L., Jordan, F. (2010) Nuclear Magnetic Resonance Evidence
for the Role of the Flexible Regions of the E1 Component of the Pyruvate Dehydrogenase Complex from Gram
Negative Bacteria J. Biol.Chem. 285 4680-4694.
217. Jordan, F. (2010) Thiamin Enzymology in Cofactor Biosynthesis and Enzymology, Ed. By Begley, T. in
Comprehensive Natural Products II Chemistry and Biology; Mander, L., Lui, H.-W., Eds.; Elsevier: Oxford, 2010;
volume 7, pp.561–598.
218. Nemeria, N.S., Arjunan, P., Chandresakhar, K., Mossad, M., Tittmann, K., Furey, W. and Jordan, F. (2010)
Communication Between Thiamin Cofactors in the Escherichia coli Pyruvate Dehydrogenase Complex E1
Component Active Centers– Evidence for a ‘Direct Pathway’ Between the 4’-Aminopyrimidine N1’ Atoms.
J.Biol.Chem. 285, 11197–11209.
219. Meyer D, Neumann P, Parthier C, Friedemann R, Nemeria NS, Jordan F, Tittmann K. (2010) Double duty for a
conserved glutamate in pyruvate decarboxylase: Evidence for participation in stereoelectronically controlled
decarboxylation and in protonation of the nascent carbanion/enamine intermediate. Biochemistry. 49(37): 8197–
8212. doi: 10.1021/bi100828r.
220. Paramasivam S, Balakrishnan A, Dmitrenko O, Godert A, Begley TP, Jordan F, Polenova T. (2011) A. Solid-State
NMR and Density Functional Theory Studies of Ionization States of Thiamin" J Phys Chem B. 115, 730-6.
PMID: 21175136 [PubMed - indexed for MEDLINE]
221. Da Jeong Shim, Natalia S. Nemeria, Anand Balakrishnan, Hetalben Patel, Jaeyoung Song, Junjie Wang, Frank
Jordan, and Edgardo T. Farinas (2011) Assignment of Function to Histidines 260 and 298 by Engineering the E1
Component of the Escherichia coli 2-Oxoglutarate Dehydrogenase Complex; Substitutions That Lead to
Acceptance of Substrates Lacking the 5-Carboxyl Group Biochemistry 50, 7705–7709. DOI: 10.1021/bi200936n
222. Balakrishnan, A; Paramasivam, S; Chakraborty, S; Polenova, T; Jordan, F (2012) Solid-State Nuclear Magnetic
Resonance Studies Delineate the Role of the Protein in Activation of Both Aromatic Rings of Thiamin
J. Am. Chem. Soc. 134, 665–672. dx.doi.org/10.1021/ja209856x |
223. Balakrishnan, Anand; Gao, Yuhong; Moorjani, Prerna; Nemeria, Natalia; Tittmann, Kai; Jordan, Frank (2012)
Bifunctionality of the thiamin diphosphate cofactor: assignment of tautomeric/ionization states of the 4'aminopyrimidine ring when various intermediates occupy the active sites during the catalysis of yeast pyruvate
decarboxylase. J Am Chem Soc. February 29; 134(8): 3873–3885. doi: 10.1021/ja211139c
224. Song, J.; Jordan, F. (2012) Interchain Acetyl Transfer in the E2 Component of Bacterial Pyruvate Dehydrogenase
Suggests a Model with Different Roles for Each Chain in a Trimer of the Homooligomeric Component. Biochemistry,
51, 2795-2803. http://dx.doi.org/10.1021/bi201614n
225. Nemeria, N.; Binshtein, E.; Patel, H.; Balakrishnan, A.; Vered, I; Shaanan, B.; Barak, Z.; Chipman, D.; Jordan, F.
(2012) Glyoxylate Carboligase: A Unique Thiamin Diphosphate-Dependent Enzyme That Can Cycle between the 4′Aminopyrimidinium and 1′,4′-Iminopyrimidine Tautomeric Forms in the Absence of the Conserved Glutamate
Biochemistry 51, 7940–7952 DOI assigned to this article is http://dx.doi.org/10.1021/bi300893v
226. Patel, H.; Nemeria, N.; Brammer, L.; Freel Meyers, C.; Jordan, F. (2012) Observation of thiamin-bound
intermediates and microscopic rate constants for their interconversion on 1-deoxy-D-xylulose 5-phosphate synthase:
600-fold rate acceleration of pyruvate decarboxylation by D-glyceraldehyde-3-phosphate. J. Am. Chem. Soc. 134,
18374−18379 http://dx.doi.org/10.1021/ja307315u
227. Balakrishnan, A.; Nemeria, N.; Chakraborty, S.; Kakalis, L.; Jordan, F. (2012) Determination of pre-steady state rate
constants on the Escherichia coli pyruvate dehydrogenase complex reveals that loop movement controls the ratelimiting step. J.Am.Chem.Soc. 134 (45) 18644-18655. http://dx.doi.org/10.1021/ja3062375.
228. Kovach, I.M.; Kakalis, L.: Jordan, .; Daoning Zhang (2013) Proton Bridging In The Interactions Of
Thrombin With Hirudin And Its Mimics. Biochemistry, 52, in press March 25, 2013.
229. Chandrasekhar, K.; Wang, J; Arjunan, P.; Sax,M. ; Park, Y-H.; Nemeria, N.S.; Kumaran, S.; Song, J.; Jordan, F.;
and Furey, W. (2013) Insight to the Interaction of the E2 Core with the Peripheral Components in the Escherichia coli
Pyruvate Dehydrogenase complex via Multifaceted Structural Approaches, J. Biol. Chem. 288: 15402-15417.
230. Jordan, F.; Patel, H. (2013) Catalysis in Enzymatic Decarboxylations: Comparison of Selected Cofactor-dependent and
Cofactor-independent Examples. ACS Catalysis 3, 1601-1617. pubs.acs.org/doi/pdf/10.1021/cs400272x; NIHMSID
#495849 Publ.ID: cs400272x
231. Balakrishnan, A.; Jordan, F., Nathan, C. (2013) Influence of Allosteric Regulators on Individual Steps in the Reaction
Catalyzed by Mycobacterium tuberculosis 2-Hydroxy-3-Oxoadipate Synthase, J. Biol. Chem. 288, 21688-21702.
doi: 10.1074/jbc.M113.465419
232. Patel, H.; Shim, D.J.; Farinas, E.T.; Jordan, F. (2013) Investigation of the donor and acceptor range for chiral
carboligation catalyzed by the E1 component of the 2-oxoglutarate dehydrogenase complex. Journal of Molecular
Catalysis B: Enzymatic J Mol Catal B Enzym. 98, 42-45. with SI.
233. Kumaran, S., Patel, M.S; Jordan, F. (2013) Title: Nuclear Magnetic Resonance Approaches In the Study of 2-Oxo acid
Dehydrogenase Multienzyme Complexes – A Literature Review. Molecules, 18, 11873-11903.
234. Hetalben Patel †, Natalia S. Nemeria †, Forest H. Andrews ‡, Michael J. McLeish ‡, and Frank Jordan (2014)
Identification of Charge Transfer Transitions Related to Thiamin-Bound Intermediates on Enzymes Provides a Plethora of
Signatures Useful in Mechanistic Studies Biochemistry 53, 2145-2152. Doi 10.1021/bi4015743, PMCID# PMC3985856
235. Wang, J., Nemeria, N.S., Krishnamoorthy, C., Kumaran, S., Arjunan, P., Reynolds, S, Calero, G., Brukh, R., Kakalis,
L., Furey, W., & Jordan, F. (2014) Structure and Function of the Catalytic Domain of the Dihydrolipoyl
Acetyltransferase Component in Escherichia coli Pyruvate Dehydrogenase Complex, J. Biol.Chem 289(22):15215-30.
doi: 10.1074/jbc.M113.544080. Epub 2014 Apr 17. PMID: 24742683 [PubMed - in process]
236. Brammer Basta, L. A.; Patel, H.; Kakalis, L.; Jordan, F.; Freel Meyers, C. L. (2014) Defining critical residues for
substrate binding to 1-deoxy-D-xylulose 5-phosphate synthase: Active site substitutions stabilize the predecarboxylation intermediate C2α-lactylthiamin diphosphate FEBS J. 281(12):2820-37. doi: 10.1111/febs.12823.
237. Patel, M.S, Nemeria, N.S., Furey, W., and Jordan, F. (2014) The Pyruvate Dehydrogenase Complexes: Structurebased Function and Regulation. J. Biol. Chem. 289(24):16615-23. doi: 10.1074/jbc.R114.563148. Epub 2014
May 5. PMID: 24798336 [PubMed - in process]
238. Jordan, F., Nemeria N.S. (2014) Progress in the Experimental Observation of Thiamin Diphosphate-bound
Intermediates on Enzymes and Mechanistic Information Derived from these Observations, Bioorganic Chemistry,
YBIOO1750, Online publication complete: 15-SEP-2014 DOI information: 10.1016/j.bioorg.2014.08.002
239. Nemeria NS, Ambrus A, Patel H, Gerfen G, Adam-Vizi V, Tretter L, Zhou J, Wang J, Jordan F. (2014) Human 2-
Oxoglutarate Dehydrogenase Complex E1 Component Forms a Thiamin-derived Radical by Aerobic Oxidation of the
Enamine Intermediate. J Biol Chem. 289, 29859-29873 pii: jbc.M114.591073. [Epub ahead of print] PMID: 25210035
240. Arjunan P, Wang J, Nemeria NS, Reynolds S, Brown I, Chandrasekhar K, Calero G, Jordan F, Furey W. (2014) Novel
Binding Motif and New Flexibility Revealed by Structural Analyses of a Pyruvate Dehydrogenase-Dihydrolipoyl
Acetyltransferase Sub-complex from the Escherichia coli Pyruvate Dehydrogenase Multienzyme Complex. J Biol
Chem. 289, 30161-30176 pii: jbc.M114.592915. [Epub ahead of print] PMID: 24742683 [PubMed - in process]
241. Wang, J., Kumaran, S., Zhou, J., Nemeria, N.S., Tao, Hu., Kakalis, L., Park, Y-H., Birkaya, B., Patel, M.S., and
Jordan, F. (2015) Elucidation of the Interaction Loci of the Human Pyruvate Dehydrogenase Complex E2·E3BP Core
with Pyruvate Dehydrogenase Kinase 1 and Kinase 2 by H/D Exchange Mass Spectrometry and Nuclear Magnetic
Resonance, Biochemistry 54 in press. dx.doi.org/10.1021/bi5013113 |
Book Edited: Thiamine: Catalytic Mechanisms and Role in Normal and Disease States, Jordan, F. and Patel, M.
Editors, Marcel Dekker, New York, pp 1-588. 2004.
Patents
1. "Production of Biologically Active Polypeptides by Treatment with an Exogenous Peptide Sequence"
US Patent 5,191,063, 3/2/1993; with M. Inouye, (RW Johnson/ Biochemistry) X. Zhu, Y. Ohta
2. " Process for Inducing Analgesia, Peptides and Therapeutic Compositions" US Patent 5,426,099, 6/20/95;
with B. Komisaruk at IAB, Rutgers University, Newark Campus; granted 6/20/95.
3. “Substituted 2-Oxo-3-Alkynoic Acids and Methods of Use Thereof” US Patent 6,204,220 B1 3/20/2001;
with Angela Marie Brown