Microbiology for Health Professionals
Fall Semester, 2014 Syllabus
Course # 34 BIOL 2031C 004
Lecture: MCDONH 145
Lab: MCDONH 245
Lab: Tues/Thurs 8:00–9:20 AM
Lecture: Tues/Thurs 9:30–10:25 AM
Faculty Service Center 513-732-5335
Dr. Nestor T. Hilvano, M.D., M.P.H.
Associate Professor of Biology
Office: MCDONH 215T; Phone: 513-558-5230
e-mail: hilvannt@ucmail.uc.edu
Office Hours: TR 10:40-12:40 PM;
By Appointment
Prerequisite:
Successful completion of a semester of freshman biology or anatomy and physiology lecture and lab with a C or better. It is
essential and assumed that all students enrolled in Microbiology course have mastered the material.
Course Description:
5 undergraduate credits. A lecture and laboratory course designed for students entering health care related fields. This
course explores the fundamental aspects of microbiology with an emphasis on those microorganisms that can cause human
disease. Topics include the structure and growth requirements of bacteria, viruses, fungi and protozoa, the methods used to
control microorganisms, microbial genetics, methods microorganisms use to cause human disease, the human immune
response, and microbial diseases of the body. No credit towards a major in biology.
Course Goals of Microbiology:
This course will explore biological processes, concepts, and theories related to microbiology. The objectives of this course
are:
1. To describe anatomy, physiology, and taxonomy of microbial organisms, with emphasis on pathogens
2. To identify microbial growth requirements, apply this knowledge to the use of antimicrobial agents, and
evaluate the effectiveness of antimicrobial agents on different populations of microorganisms.
3. To identify the methods by which microorganisms obtain genetic change, to apply this knowledge to
describing how new strains of microorganisms arise, and to evaluate the potential impact of this genetic
change on the treatment and prevention of microbial diseases.
4. To describe the methods by which microorganisms cause disease, with emphasis on virulence factors and
transmission of disease and apply this knowledge to methods used to prevent transmission of infectious
agents
5. To identify the functions of the immune response and other natural defense mechanisms and apply this
knowledge to controlling and preventing human infections
Methods of Reaching These Objectives and Learning Outcomes:
Students need to prepare for class by reading and thinking about scheduled topics prior to class time. Before arriving for
class, they should have a basic understanding of the topics to be discussed and have formulated any questions that might
have arisen as they read about these topics. This will enable the students to participate meaningfully in discussing the
scheduled topics for that day.
Learning Outcomes
At the completion of this course, students should be able to:
1.
Accurately define key terminologies.
2.
Translate pertinent wordstems.
3.
Reproduce explanatory diagrams and illustrations.
4.
Explain important concepts in their own words.
5.
Develop learning skill in case-based discussion.
6.
Apply the importance of microbes in diseases including its transmission and control.
Required Text:
Bauman, Robert. 2009. Microbiology, With Diseases by Taxonomy, 3rd edition, Pearson Benjamin Cummings, San
Francisco CA.
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Required Equipment:
1.
A bound 10 × 7.875 inch composition notebook with graph rulings, hard cover and relatively thick pages. All notes
taken during lab need to be entered DIRECTLY into this notebook.
2.
A pen, such as “Tombo Roll Pen, Jr. ®” or Rapidiograph, which writes with BLACK indelible (waterproof) ink.
Water-soluble markers, ballpoint pen (which is soluble in alcohol, etc.), and felt-tips, which “bleed” through the page, are
NOT acceptable.
3. A set of colored pencils and lead pencils.
Final Exam Period: Dec. 8- 13, 2014
Grading:
The final grade for the course is a composite of all grades earned in lecture and laboratory. It is based on the total number of
points from the three best of four exams (100 pt. each), the three best of four quizzes (25 pt. each), up to ten lab exit/pop
quizzes (5 - 10 points each), final exam (200 pt.), laboratory notebook (200 pt.), 20 pt. penalty each for each lab absence
will be deducted on lab notebook. Ten percent per class period will be deducted for any work turned in late. Cheating will
earned a failing grade (F). Grades will be awarded based on an A (94-100), A- (90-93), B+ (87-89), B (84-86), B- (80-83),
C+ (78-79), C (75-77), C- (70-74), D+ (68-69), D (66-67), D- (64-65), F (63 and below) grading scale.
Exams and Quizzes:
There will be four exams worth 100 pts. each, four quizzes of 25 pt. each, lab exit/pop quizzes of 5-10 pt. each, and a final
exam worth 200 pts. Exam and quiz structure will require written essays and/or short answers. A histogram (curve) of test
scores will be constructed and analyzed using statistical methods. However, the dividing line between “B” and “C” will not
be adjusted down below the 65% mark and the full grade difference will not be more than 10. No make-up quizzes, tests or
final exams will be available. Exams and quizzes will cover materials from each lecture and lab session, and grades will
not be adjusted for any lectures or labs students miss. In addition, once a student accepts an exam, (s) he must finish it
during that class period. Students will not be allowed to decide halfway through an exam that they are under a physicians
care and cannot complete the exam.
Lecture and Laboratory Session Attendance:
Lecture and lab session attendance is mandatory. Perfect attendance will earn 20 pts. Extra credit. Attending weddings,
going on family vacations, having to work, not having a babysitter, or alike are not valid excuses to miss a lecture or lab
session. There is an automatic 20 pt. penalty for any missed laboratory session and, because of limited availability of our
laboratory, lab sessions cannot be made up. Students missing more than 3 lab sessions will receive an automatic F for
the course.
Lab Attire:
Please be aware that you will NOT be allowed to attend lab unless you are wearing long pants (leggings if wearing a
dress) and you have on shoes that cover the ENTIRE top of your feet and the heel. Shoes cannot have any holes in
them. That means NO flip-flops, crocs, slippers, etc. Also be aware that we may be using Bunsen burners. Plan on pulling
back long hair. You may want to limit clothing that is excessively loose or dangling. We will also be using permanent
stains, so you may want to wear old clothing during those labs. Some labs require gloves. You must purchase the gloves
in the UC Bookstore before coming to class.
Food and Drinks:
Eating and drinking in the labs are not allowed.
Withdrawals:
This course will adhere to the Standard University of Cincinnati Policy. Students will be able to withdraw from classes online. The Web Registration site will remain active through the 58 th day of the quarter – for withdrawals only. When students
submit their withdrawal, they will receive an automatic notification that the withdrawal was submitted. They will also
receive a follow-up e-mail notifying them. Faculty will also receive an e-mail notifying them of the web withdrawal.
Faculty can still change the “W” to an “F” if that is a more appropriate grade, when they do their final grades. Last day to
drop (no entry to academic record) and the last day to submit Grade Replacement Forms is September 8, 2014. Last
day to withdraw from classes is October 31, 2014
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Academic Integrity Policy:
The University Rules, including the Student Code of Conduct will be enforced. Any violation of these regulations,
including acts of plagiarism or cheating, will be dealt with on an individual basis according to the severity of the
misconduct.
Plagiarism: (likely to result in expulsion from the Clermont College)
1. Submitting another's published or unpublished work, in whole, in part, or in paraphrase, as one's own without fully and
properly crediting the author with footnotes, quotation marks, citations or bibliographical references.
2. Submitting as one's own, original work, material obtained from an individual, agency, or the internet without reference to
the person, agency or webpage as the source of the material.
3. Submitting as one's own, original work, material that has been produced through unacknowledged collaboration with
others without release in writing from collaborators.
4. Submitting one’s own previously written or oral work without modification and instructor permission.
ADA:
Students with Disabilities: The Policy of the University of Cincinnati Clermont College requires students to self-identify
and provides proper documentation to Jennifer Radt, 732-5327, Academic Director of Disability Services, located in the
Student Services Building.
The Learning Center
FREE tutoring is available at The Learning Center (CCMCDONH 100) to all UC Clermont students. TLC regular hours
are: Mon - Thurs 7:30am - 6:30pm and Friday 7:30am - 4:00pm. Walk-in for assistance, call 513/732/5228 or email clcleaningcenter@uc.edu to make an appointment with a tutor.
Find out more on TLC website
http://www.ucclermont.edu/TLC and The Learning Center organization on Blackboard.
College Success Program
Entering degree-seeking students, experiencing academic, social, or personal difficulties that can impede their success, can
receive additional help at no cost from the College Success Program (CSP). For more information, contact Lesley Dorhout,
Achievement Coach (732-5316; lesley.dorhout@uc.edu).
Visitors:
I realize that some students may have problems arranging for childcare. However, University of Cincinnati Clermont
College does not allow children to be left unattended. In addition, the Ohio Environmental Protection Agency, the
Occupational Safety & Health Administration, and the University of Cincinnati Environmental Health & Safety Department
prohibit the presence of minors in science laboratories for safety reasons, unless minors are enrolled as students in
laboratory courses taught in those rooms.
Electronic Gadgets:
All beepers or cell phones are to be turned off and put inside the bag (backpack) before entering the class. Also answering
phone calls or text messaging in lecture or lab is not permitted. There are no exceptions.
Contact by Phone or E-Mail: Students are encouraged to contact me by phone or email to have important questions
answered. However, any contact must occur in a professional and courteous manner.
Acknowledgement:
The contents of this course have been designed to follow Dr. David Fankhauser’s Microbiology course. I gratefully
acknowledge the use of Dr. Fankhauser’s excellent lab protocols for our students.
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Microbiology (BIOL2031C004) Lecture Schedule, TR 9:30-10:25 AM
MCDONH 145
Week
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Tuesday (T)
Aug. 26 Introduction: History of Microbiology
Sept. 2 Germ Theory of Disease; Microscopy and
Staining
Sept. 9 Quiz 1 (Intro to Microscopy and Staining);
Prokaryotic Anatomy part 1
Sept. 16 Test 1 (Intro to Prokaryotic Anatomy part 2)
Thursday (R)
Aug. 28 Spontaneous Generation =
Sept. 4 Microscopy and Staining
Sept. 11 Prokaryotic Anatomy part 2
Sept. 18 Microbial Genetics
Sept. 23 Microbial Metabolism, Glycolysis,
Sept. 25 Microbial Growth and Nutrition
Fermentation
Lab Notebook Due (50 pts.)
Sept. 30 Quiz 2 (Microbial Genetics to Microbial
Oct. 2 continue Physical Means of Microbial Control
Growth and Nutrition); Microbial Control: Physical
Means
Oct. 7 Test 2 (Microbial Genetics to Physical Means
Oct. 9 No Classes, Fall Reading Day
of controlling microbial growth)
Oct. 14 Microbial Control: Chemical Means
Oct. 16 Microbial Control: Chemical Means
Lab Notebook Due (50 pts.)
Oct. 21 Quiz 3 (Chemical Means of Microbial
Oct. 23 Gram Positive Bacilli; Bacillus, Clostridium
Control); Gram Positive Cocci: Staph & Strep
Oct. 28 Test 3 (Chemical Means of Microbial Control Oct. 30 Gram Negative Bacteria; Neisseria,
to Gram Positive Bacilli)
Enterobacteriaceae
Nov. 4 Mycoplasma, Rickettsias, Chlamydias,
Nov. 6 Quiz 4 (Gram Negative Bacteria to Vibrio);
Spirochetes, and Vibrios
Fungi and Parasites
Nov. 11 Holiday, Veteran’s Day
Nov. 13 Fungi and Protozoa
Nov. 18 Test 4 (Gram Negative Bacteria to Fungi and Nov. 20 Viral Diseases Part 1
Protozoa)
Nov. 25 Viral Disease Part 2
Nov. 27 No Classes- Thanksgiving Holiday
Lab Notebook Due (100 pt.)
Dec. 2 Disease Patterns and Epidemiology
Dec. 4 Host Defenses and Immunology
Final Exam Week: Dec. 8 – 13, 2014 (Final Exams TBA)
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Microbiology (BIOL2031C004) Lab Schedule, TR 8:00-9:20 AM
MCDONH 245
Revised Fall 2014
Week
Tuesday (T)
1
Aug. 26 Lab Introduction and Safety; Keeping a Lab
Notebook
2
Sept. 2 Bacterial Morphology (3 Types of microbes at
400X); Bacterial Prepared Slides part 1 (2-6)
3
Sept. 9 Bacterial Growth Curve; Spectrophotometer
Use; Semi-log graph
4
Sept. 16 Gram Stain Protocol; Milk Fermenters
5
Sept. 23 Sterile Technique; Yeast Plate Count
Lab Notebook Due (50 pts.)
6
Sept. 30 UV Killing of Bacteria; Growth Inhibition,
Antibacterial Potency
7
Oct. 7 Epidemiology
8
Oct. 14 Beta Hemolytic Strep Detection by Throat
Culture; Blood Agar Use
Lab Notebook Due (50 pts.)
9
Oct. 21 Enumeration by Pour Plates: Meat & Milk
10
11
12
13
14
15
Oct. 28 Bring in Liquid Samples;
Assay for Coliform Contamination in Ambient Water
Nov. 4 Record Results; TSI Inoculation
Nov. 11 Holiday, Veteran’s Day
Thursday (R)
Aug. 28 Microscope Care and use: Letter “E” and
Buccal Smear
Sept. 4 Bacterial Smear & Staining: Simple Staining;
Wet Mount
Sept. 11 Prepare Solid Media; Autoclave Use
Sept. 18 Serial Dilutions, Pipetting/Dilution Problems
Sept. 25 Count Plates/Calculations
Oct. Record Results of UV killing/Growth Inhibition
Oct. 9 No Classes, Fall Reading Day
Oct. 16 Check Blood Agar Plates Results
Oct. 23 Count and Record Results;
Take home sterile tube to collect liquid samples
Oct. 30 Count Coliform Plates; Single Colony
Isolation
Nov. 6 Check and Record TSI Slant Preps
Nov. 13 Prepared Slides part 2 (9-14); Take home
bottle for water collection
Nov. 20 Count Membrane Filters
Nov. 18 Bring in Drinking Water: Membrane Filter
Assay- Coliform in Drinking Water Assay
Nov. 25 Protozoans and Helminthes Parasites
Nov. 27 No Classes- Thanksgiving Holiday
Lab Notebook Due (100 pt.)
Dec. 2 Protozoans and Helminthes Parasites
Dec. 4 Review/Catch up/Lab Clean Up
Final Exam Week: Dec. 8 – 13, 2014 (Final Exams TBA)
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