MEDICAL UNIVERSITY OF BIALYSTOK
DEPARTMENT OF MICROBIOLOGY
Mickiewicza 2 C, BIALYSTOK 15-222
tel./fax +48 (85) 748 5562
Course: Microbiology
Program of classes: 2015 / 2016
Winter/Summer semester (V-VI) - 15 weeks
[45 didactic hours (labs) + 3 tests (5 didactic hours); labs duration – 2.25 clock hours]
Winter semester (V) (Lectures/Labs 1-3)
Location: Classroom at the Department of Microbiology
(Collegium Universum, Mickiewicza 2c Street)
Time:
1st and 3rd group
2nd group
Thursday: 9.45 – 12.10
Friday: 11.00 – 13.25
Lab
I. Study of the microbial cell morphology under the light microscope.
Tutorial :
1. Organizational matters.
2. Safety precautions in microbiology laboratory.
3. Microscopy and various types of microscopes.
4. Bacterial cell morphology and bacterial staining methods (Gram staining method).
Date
14 & 15 January
2016
Practical part:
Evaluation of microbial cell morphology under the light microscope and practice of Gram
staining technique.
Recommended reading:
“Medical Microbiology” Murray PR:
5th edition: Chapter 3 – “Bacterial morphology…” (pp.11-12) and Chapter 16
6th edition: Chapter 3 – “Bacterial classification…” (pp. 9-10) and Chapter 14
II. Bacteria staining techniques and methods for isolation and cultivation of
aerobic bacteria.
Student's knowledge evaluation:
Topics from the 1st Lab and Lecture and the related book chapters
(“Medical Microbiology” Murray PR, 5th ed.: Chapter 1 – “Introduction to Medical
Microbiology” and 2 – “Bacterial classification”)
Tutorial:
1. Division and application of microbial staining methods:
- simple vs. diffrential staining; positive vs negative techniques
- special staining techniques (Neisser´s, Dorner´s, Maneval´s, Ziehl-Neelsen stain, etc.)
2. Microbiological media and methods for isolation and cultivation of aerobic bacteria.
- liquid and solid media, simple and enriched media, differential and selective media
- microbial cultures (mixed vs. pure) and growth conditions
21 & 22 January
2016
- microbial colony and cultural appearances
Practical part:
1. Microscopic observation of bacterial capsules, spores and granules.
2. Isolation of microorganisms from clinical specimens on solid media (practice of the
quadrant streak method).
3. Gram staining and microscopic observation of preparations from clinical specimens.
4. Recognition of microorganisms growing on solid media.
Recommended reading:
“Medical Microbiology” Murray PR, 6th ed.: Chapter 15 – “In vitro culture…”
III. Bacteria identification methods, cultivation of anaerobic and
microaerophilic bacteria.
28 & 29 January
2016
Student's knowledge evaluation:
Topics from the Lab 2 and Lectures 2-3 and related book chapters:
(“Medical Microbiology” Murray PR: Chapters 3-5 (5th ed.): “Bacterial morphology…”,
“Bacterial metabolism and growth” and “Bacterial genetics”
or Chapters 3-4 (6th ed.))
Tutorial:
1. Classification and characteristics of anaerobic and microaerophilic bacteria.
2. Media and methods for isolation and cultivation of anaerobic and microaerophilic
bacteria.
Practical part:
1. Bacterial growth in liquid media.
2. Identification of microorganisms present in clinical samples on selective and differential
media (a completion of the experiment from the previous lab).
3. Macroscopic observation (colony morphology) of bacterial cultures, including
anaerobic and microaerophilic bacteria.
4. Gram staining of anaerobic and microaerophilic bacteria.
Recommended reading:
“Medical Microbiology” Murray PR, Chapter 4 – “Bacterial metabolism and growth” (5th
ed.) or Chapter 3 (6th ed.)
TEST 1: General microbiology
Covers topics from Labs/Lectures 1-3 and the related book chapters
1st week after the
winter break
Recommended Books:
Basic:
1. Murray PR., Rosenthal KS., Pfaller MA. “Medical Microbiology”. 6th ed.. MOSBY. Elsevier 2009, or 5th ed, 2005.
2. Murray PR., Rosenthal KS. “Review of Medical Microbiology”. Elsevier MOSBY, 2005
Optional:
1. Brooks G.F., Butel J.S., Morse S.A. “Jawetz, Melnick, & Adelberg's. Medical Microbiology”
2. Levinson W. “Medical Microbiology & Immunology: Examination & Board Review”.
3. Harvey RA. “Lippincott's Illustrated Reviews: Microbiology”
The course coordinator: Krzysztof Fiedoruk, MSC, PhD