Name - _________________________________________________
Microbiology Test #1 Review Guide
1.
Define microbiology.
2.
Which characteristics make microorganisms suitable for laboratory study?
3.
List and describe Koch’s postulates for the etiology of a disease.
1.
2.
3.
4.
4.
What unit of measure would most commonly be used to describe the size of a bacterial cell?
5.
What unit of measure would most commonly be used to describe the size of a Virus?
6.
Create a chart for the following (drawings are helpful):
a.
Basic cell shapes commonly found among bacteria
b.
The basic cell arrangements commonly found among bacteria
7. List general characteristics commonly used to identify and/or classify bacteria.
8.
Define / describe each of the following as they apply to microbiology:
a. Culture
b. Inoculum
c. Inoculation
d. Colony
e. Growth medium (-ia)
f.
Pure culture
g. Contaminated culture
9.
Microbiologists employee a number of approached to acquiring a pure culture from a sample containing a number of different types
of bacteria. Briefly describe procedures commonly used to secure pure cultures from a mixed culture. The use of simple labeled
diagrams may be quite helpful.
10. Complete the following table of units of measure commonly used in microbiology.
Unit
Abbreviation
Exponential Value
1 centimeter
1 nanometer
cm
mm
10-3
μm
10-6
nm
11. Describe the procedure for making a bacterial smear for microscopic observation (don’t forget aseptic technique!).
12. Why should a bacterial smear be air-dried before heat fixing (“flaming”) it? What is the purpose/effect of flaming a bacterial smear
slide?
13. Describe the procedure for making a gram stain (don’t forget aseptic technique!).
14.
Define:
Microbiology
Virology
Mycology
Parasitology
Protozoology
Phycology
15. BRIEFLY summarize the major accomplishments of the microbe hunters: