Houston Community College Microbiology (Biol. 2420) IN-Person LABS Required Lecturer: Dr. Perry CRN 49420 Textbook: Kathleen Park Talaro. 2006. Microbiology (6th edition). Wm. C. Brown Publishers/ McGraw Hill. Lab Manual: A Laboratory Manual: Microbiology HCCS Publishing. NOTE: Textbook and Lab Manual are both required Hints for Success : Read all of the assigned chapters as early as possible. Outline all chapters for understanding. Review your notes often for the exams. This increases your passing rate and grade. Class Rules 1. Email me at beverly.perry@hccs.edu as soon as you have read these instructions, so that I will have your correct email address and an alternate way of communication. Tell me about yourself, your goals and anything else that you want me to know. 2. Put your cell phone on vibrate before entering the room. No cell phones may sound in my class. I will take off 10 points from your lecture exam grade every time that this happens. 3. No gum chewing in class. 4. Do not disturb or interrupt the instructor during class for any reason. 5. Raise your hand to be recognized and wait until you have permission to talk. 6. You are always free to go to the restroom during lab time. 7. You must place ALL lab items back into the cabinets and clean up. When you finish, you may leave. 8. Any absence should be discussed with the instructor. 9. I also prefer this email address to the class email. I read this one several times a day. 10. I also prefer that you call me with your concerns at 713-718-8048. Leave a brief message. URGENT NOTICE: Take the chapter exams only when you are supposed to have access and are ready. Lecture coverage is done by self-study utilizing online course resources along with the textbook are allowed. Do not open the lecture exams until it is time for you to take it because the clock starts on the test and you cannot re-enter it. Timed exams can only be attempted once. No exceptions or redos allowed. Once you have opened an exam, you cannot leave it or go over the allotted time. No second chances at tests will be given. F will be given for that test. Students who miss 2 labs for any reason are automatically dropped from the course. Students who miss any exam are automatically dropped from the course. CLASS ACTIVITIES/ SYLLABUS (Textbook Chapters are in bold print ) 1 The Main Themes of Microbiology ORIENTATION TO LAB and Distance Education 3 4 5 6 Tools of the Laboratory: Methods for Studying Microorganisms (continued) Prokaryotic Profiles: The Bacteria and Archaea Lab. Exercise 1: Care and Use of the Microscope Friday, June 8 Lab. Exercise 2: Smear Preparation, Simple Staining and Bacterial Morphology Lab. Exercise 3: Negative Staining Method Eukaryotic Cells and Microorganisms An Introduction to the Viruses Lab. Exercise 4: Isolation of Bacteria Lab. Exercise 5 Gram Staining Lab. Exercise 6: Acid-fast Staining First Lecture Exam (Chapters 1, 3, 4) 7 9 Friday, June 8 Friday, June 15 June 20-23 Friday, June 22 Lab. Exercise 7: Bacterial Endospores Lab. Exercise 8: Bacterial Capsules Lab. Exercise 9: Bacterial Flagella and Motility Testing Elements of Microbial Nutrition, Ecology, and Growth Microbial Genetics Friday, June 29 Lab. Exercise 10: Selective, Differential and Enriched Media Lab. Exercise 11: Gas Requirements for the Growth of Bacteria Lab. Exercise 12: Counting Bacteria in Milk using the Pour Plate Method Lab. Exercise 13: Effects of Heat Second Lecture Exam (Chapters 5, 6, 7,9) July 11-13 Lab Exercise 14: Effects of Chemicals Lab. Exercise 15: Carbohydrate Fermentation by Bacteria Lab. Exercise 16: Triple Sugar Iron (TSI) Fermentation Lab. Exercise 17: The IMViC Biochemical Tests Friday, July 13 10 Genetic Engineering: A Revolution in Molecular Biology 11 Physical and Chemical Control of Microbes 12 Drugs, Microbes, Host----The Elements of Chemotherapy 14 15 Friday, July 20 Lab. Exercise 18: Rapid and Miniaturized Methods of Identification Of Bacteria Lab. Exercise 19: Bacteriophages Lab. Exercise 20: Immune System: White Blood Cells Lab. Exercise 21: Antibiotic Sensitivity Testing: Kirby-Bauer Method Midterm Lab Exam. (Tentative) 13 Microbe----Human Interactions: Infection and Disease The Nature of Host Defenses The Acquisition of Specific Immunity and Its Applications Lab17-21 View lab results and complete reports Friday, July 27 Third Lecture Exam (10, 11, 12, 13) 16 17 July31-Aug 1 Immunization and Immune Assays Disorders in Immunity Lab Final Exam Lecture Final Exam (Chapters 14, 15, 16, 17) August 3 August 7 NOTE: This is a tentative schedule and subject to modification. You will be notified by email and reminded in lab of such changes. I am honored to have you in my class. Call me at 713-718-8048 for any issues. Use my preferred email address.