Title: Factors Affecting Enzyme Activity
• Problem: How do enzymes help maintain
homeostasis and what factors affect how an
enzyme will work?
• Hypothesis: What factors will affect enzyme
activity the most?
• Procedure: Summarize the procedure (see
handout).
• Data/Observations: Record all observations you
see. Construct a data table (use a ruler) to display
ALL of your data.
• Analysis/Conclusion: Answer questions in
complete sentences.
Graph(s)
• Construct graph(s): Be sure to include the
following data:
•
1. Rate of enzyme activity vs. temperature
•
2. Rate of enzyme activity vs. pH
•
3. Rate of enzyme activity vs. surface area
•
**Be sure to label x & y axis’s, have a
relevant title, use a ruler & make it nice and
neat!
Analysis & Conclusion
Discussion:
1.
2.
3.
4.
5.
6.
7.
8.
What is an enzyme? How is it useful?
What is an active binding site? How is it related to a substrate?
What is a substrate?
In this lab, adding hydrogen peroxide to the liver yields what products?
Are enzymes reusable? Explain using data from your lab to support your explanation.
What are some factors that affect enzyme activity?
What will boiling do to an enzyme? Support your answer with data from the lab.
How do you explain the difference in activity resulting from the whole piece of liver
and from the ground liver.
9. What is the effect of various temperatures on enzyme activity?
10. What is the effect of various pH solutions on enzyme activity?
Conclusion:
•
•
Restate your hypothesis. Accept OR reject it. Be sure to use DATA to support!
Draw conclusions about the factors that influence enzyme activity.
Apply Your Knowledge
• Explain (using evidence AND data from
your lab) why heat stroke is potentially life
threatening.
• What can we do to prevent heat stroke?