Enzyme Lab
This is what you need for the enzyme lab:
I: Purpose: To determine if the enzyme, catalase, is present in different materials.
II: Hypothesis: Predict which materials (chalk, liver, spinach) will have catalase.
III: Materials and Procedure: List the materials used in lab. Then, describe the procedures (in
detail) of how you conducted the lab.
IV. Data/Results: Make a bar graph showing the level of reaction (bubbling) for each of the 3
materials. Then, provide qualitative and quantitative observation of the experiment.
V. Conclusion: Was your hypothesis wrong/right? Why? Describe your data. What did you
learn?
You also need to include these questions/answers:
This chemical equation may help: 2H2O2  H2O + 2O2
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Which of the substances contained more of the enzyme? Look at your graph.
If a substance created no reaction, explain why?
What 2 products does hydrogen peroxide change into when it breaks down?
What is the function of an enzyme?
What enzyme acts on hydrogen peroxide in living organisms?
Would this enzyme act on chemicals other than hydrogen peroxide? Why or why not?