Information Request Form
09/2011
High-throughput RNAi Screening Platform
Confidential
High-throughput RNAi Screening Platform
Information Request Form
Your screening assay is certainly different from others and needs to be set up in a special way.
This preparation form helps us to understand the biological background of your screen and
your individual needs.
Please fill out the questions below to the best of your ability. If you do not know the answer,
just type in “not known.” Please email the form to ulrike.hardeland@medma.uni-heidelberg.de
and schedule an appointment to go through your screening needs.
In the mean time, feel free to contact us to get a tour of the lab and equipment and to discuss
your assay protocol as well.
See http://rnai-screening-wiki.dkfz.de/signaling/wiki/display/rnaiwiki/ for more information.
Contact Information
First and Last Name:
Title:
Telephone Number:
Email:
P.I. Name:
Department and Division:
Lab location:
Screening Goals
a. What is the aim of the screen?
b. What is envisioned the timeline for completing your screen?
c. Do you have any 96 or 384 well plate automation or instrumentation experience?
Organisms
a. Drosophila cells
b. Human cells
Page 1 of 3
Information Request Form
09/2011
High-throughput RNAi Screening Platform
Confidential
1. Assay development
a.
b.
c.
d.
Please briefly describe your (ideal) protocol or what you plan to do.
What is the output of the assay?
Have you done this assay before?
Can you provide data with positive and negative controls that demonstrate your
assay works?
2. Readout
a.
b.
c.
d.
e.
f.
Are you running an imaging or plate reader assay?
If imaging, can you provide example images?
What detection configuration do you need (filters, magnification, etc.)?
What label(s) are you using?
Have you optimized your staining conditions?
What readout or phenotype do you want to screen for?
3. Cell lines
a.
b.
c.
d.
What cell line(s) are you using?
What organism?
Do you know what your ideal plating density/confluency is?
Have you optimized your transfection conditions?
4. Controls
a. Please list your positive controls and describe the phenotype.
b. Please list your negative controls and describe the phenotype.
5. Plate type
a.
b.
c.
d.
Have you attempted to run your assay in a 96 or 384 well format?
What plate type (brand and number) do you use?
If so, have you optimized your seeding density for the plate you use?
Is there anything special about this plate, such as coating, or can you consider a
substitute?
e. Have there been cell adherence issues when performing pipetting or other
manipulations in the plate used?
6. Timing
a. Have you optimized the incubation timing (cells/controls) of your assay?
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Information Request Form
09/2011
High-throughput RNAi Screening Platform
Confidential
7. Library
a. What screening library(s) do you intend to use?
8. Data Analysis
a. How is the data analyzed?
b. Do you need support by the High-throughput RNAi Screening Platform for
analyzing your data?
9. Administrative
a. Is the screen funded?
b. What is your cost estimate?
10. Miscellaneous
Correspondence to:
Dr. Ulrike Hardeland
University of Heidelberg
Medical Faculty Mannheim
Department Cell and Molecular Biology
Ludolf-Krehl-Strasse 13-17
D-68167 Mannheim
Germany
Phone: +49 (0)621 383 9648
Fax: +49 (0)621 383 9652
Email: ulrike.hardeland@medma.uni-heidelberg.de
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