Preliminary Program
"High Content Assay for Target Discovery using RNAi Technology"
SUNDAY MAY 10TH
- Arrival in Korea
MONDAY MAY 11TH
Designing & establishing cell-based assays for screening
- Registration and Breakfast (8:30AM to 9:30AM)
Theoretical course
- Introduction to high content analysis and screening (HCA/HCS)
- Cell culture techniques towards high throughput screening assays.
- Assay development and optimization
Practical training
- Practice on 384 well plate cell culture
- Performing a pilot screen with appropriate controls
TUESDAY MAY 12TH
Designing & setting up assays for RNAi screening (siRNA, miRNA, & shRNA) & High Content Assay:
instrumentation and assay development
Theoretical course
- Overview of RNA interference methods
- Selection of assay controls for quality control, data visualization and analysis
- Adapting your assay to your instrumentation
Practical training
- Practice on HCS instrumentation technologies
WEDNESDAY MAY 13TH
Automated microscopy image processing and analysis
Theoretical course
- Overview of the existing software for processing of biological images
- Introduction to image processing and analysis (filtering, segmentation etc.)
- Identifying acquisition problems and solutions (out of focus, biased illumination, etc...)
Practical training
- Image processing and analysis of large-scale studies
THURSDAY MAY 14TH
RNAi screening data analysis for gene nomination, data management, and data mining
Theoretical course
- Managing RNAi screening data sets.
- Identification of systematic errors potentially occurring during screening
- Identifying and controlling for off-target effects.
- Overview of tools to classify, cluster and identify phenotypes of interest among large and
multivariate data.
Practical training
- Practice on large scale RNAi screening data analysis
FRIDAY MAY 15TH
RNAi data analysis in a cellular pathway context
Theoretical course
- Method and database to explore cellular pathways.
Practical training
- Practice on RNAi data analysis in a cellular pathway context
Presentation by each student group of their experimental results
Course evaluation
Course conclusion
SATURDAY MAY 16TH
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Departure
Notes: On Monday morning, students should give a 5 minute PowerPoint presentation on the question
they have and what they want to do with the HCS siRNA technology. On Friday students will have the
evaluation.